Detailed information of TA system TA076597

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafQ-RelB
• Location: 1328931..1329497
• Replicon: chromosome
• Accession: NZ_CP021394
• Organism: Bifidobacterium breve strain NRBB56

Toxin (Protein)

• Gene name: relE
• Uniprot ID: W6EX94
• Locus tag: NRBB56_RS05990
• Protein ID: WP_003831807.1
• Coordinates: 1329204..1329497 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: NRBB56_RS05985
• Protein ID: WP_025221722.1
• Coordinates: 1328931..1329200 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NRBB56_RS05960	1324679..1325375	-	697	Protein_1106	response regulator transcription factor	-
NRBB56_RS05965	1325501..1326016	+	516	WP_065452570.1	hypothetical protein	-
NRBB56_RS05970	1326013..1326903	+	891	WP_003831809.1	hypothetical protein	-
NRBB56_RS05975	1326900..1327580	+	681	WP_106630477.1	ABC transporter ATP-binding protein	-
NRBB56_RS05980	1327577..1328746	+	1170	WP_106630478.1	ABC transporter permease	-
NRBB56_RS05985	1328931..1329200	+	270	WP_025221722.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
NRBB56_RS05990	1329204..1329497	+	294	WP_003831807.1	type II toxin-antitoxin system YafQ family toxin	Toxin
NRBB56_RS06000	1330166..1330744	+	579	WP_106630760.1	RNA polymerase sigma factor	-
NRBB56_RS06005	1330751..1331578	+	828	WP_106630479.1	hypothetical protein	-
NRBB56_RS06010	1331711..1332337	+	627	WP_106630480.1	hypothetical protein	-
NRBB56_RS06015	1332334..1333116	+	783	WP_106630481.1	murein hydrolase transporter LrgB	-
NRBB56_RS06020	1333168..1334043	+	876	WP_106630482.1	ABC transporter ATP-binding protein	-
NRBB56_RS06025	1334098..1334496	+	399	WP_015438839.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1308610..1348660	40050

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-95)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (3-88)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11218.98 Da
• Isoelectric Point: 9.7881

>T76597 WP_003831807.1 NZ_CP021394:1329204-1329497 [Bifidobacterium breve]
MLKPDYTAAFQRDIKKLKRKHTDLRPLKEVIRLVLEDTADSKEVLRRRHRAHTLTGDLNGVLECHIGNAGDWLLLWIRDD
GTAMFMRTGSHDELLGK

Nucleotide

• Length: 294 bp

>T76597 NZ_CP021394:1329204-1329497 [Bifidobacterium breve]
ATGCTCAAACCGGATTACACAGCGGCATTCCAGCGAGATATCAAGAAACTCAAACGCAAGCACACTGATTTACGCCCGCT
GAAAGAAGTCATTCGTCTGGTTCTTGAGGACACGGCGGACTCCAAGGAAGTATTGAGGCGTAGGCATCGCGCCCACACAC
TTACCGGTGATTTAAACGGCGTACTGGAATGCCACATCGGCAATGCCGGCGACTGGCTGCTTCTATGGATTCGCGATGAT
GGTACCGCCATGTTCATGCGAACCGGCAGCCATGACGAACTGTTGGGCAAATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9859.22 Da
• Isoelectric Point: 6.3186

>AT76597 WP_025221722.1 NZ_CP021394:1328931-1329200 [Bifidobacterium breve]
VSMASVTVRVDADTKQKAANIAEDLGLDLSSVTRAFYKQIVRERRIPLNLSYTTVPPETMKALDEAKNRLADGKPRFNSA
DEMFDSLGI

Nucleotide

• Length: 270 bp

>AT76597 NZ_CP021394:1328931-1329200 [Bifidobacterium breve]
GTGAGCATGGCATCCGTCACAGTCCGCGTGGATGCGGACACCAAGCAGAAAGCCGCAAACATAGCCGAGGATCTCGGTCT
GGACTTGTCTTCGGTCACCCGCGCCTTCTACAAGCAGATTGTCCGGGAGAGGCGCATTCCTCTAAACCTTTCCTATACGA
CCGTGCCGCCTGAAACCATGAAGGCCCTTGACGAGGCGAAAAACAGGCTCGCCGACGGCAAACCTCGTTTCAACAGTGCC
GATGAGATGTTCGATTCGTTGGGAATCTGA

Structures

Toxin

• AlphaFold DB: W6EX94