Detailed information of TA system TA076596

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/DinJ(antitoxin)
• Location: 1149835..1150471
• Replicon: chromosome
• Accession: NZ_CP021394
• Organism: Bifidobacterium breve strain NRBB56

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A0M3T8E7
• Locus tag: NRBB56_RS05100
• Protein ID: WP_052787173.1
• Coordinates: 1149835..1150191 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: NRBB56_RS05105
• Protein ID: WP_016463214.1
• Coordinates: 1150178..1150471 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NRBB56_RS05070	1145024..1145935	+	912	WP_106630435.1	ABC transporter ATP-binding protein	-
NRBB56_RS05075	1146132..1146758	+	627	WP_106630436.1	30S ribosomal protein S4	-
NRBB56_RS05080	1147095..1147877	-	783	WP_106620802.1	gamma-glutamyl-gamma-aminobutyrate hydrolase family protein	-
NRBB56_RS05085	1148020..1148505	-	486	WP_003829229.1	cytidine deaminase	-
NRBB56_RS05090	1148595..1148951	+	357	WP_003833231.1	SdpI family protein	-
NRBB56_RS05095	1149056..1149658	+	603	WP_003829233.1	transglutaminase family protein	-
NRBB56_RS05100	1149835..1150191	-	357	WP_052787173.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
NRBB56_RS05105	1150178..1150471	-	294	WP_016463214.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
NRBB56_RS05115	1150903..1151577	+	675	WP_015438748.1	histidine phosphatase family protein	-
NRBB56_RS05120	1151660..1152043	+	384	WP_016463075.1	DUF948 domain-containing protein	-
NRBB56_RS05125	1152043..1152279	+	237	WP_003829239.1	hypothetical protein	-
NRBB56_RS05130	1152375..1155053	+	2679	WP_106630437.1	alanine--tRNA ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-110)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (11-53)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13313.23 Da
• Isoelectric Point: 7.9984

>T76596 WP_052787173.1 NZ_CP021394:c1150191-1149835 [Bifidobacterium breve]
MMKTDPHQFEIWWVPFAFPDKPGKAKNRPSVILQWDDGTRIALVTKVTGNTWRDEPGYVVLRDWQDAGLSKPSAVRCSQL
LRLPSNLFLDDGPTGRLSAYDAGRVTWAINELYPGLLA

Nucleotide

• Length: 357 bp

>T76596 NZ_CP021394:c1150191-1149835 [Bifidobacterium breve]
ATGATGAAGACTGATCCGCACCAGTTCGAGATATGGTGGGTGCCGTTCGCTTTTCCCGACAAGCCAGGCAAAGCGAAGAA
CCGTCCCAGCGTGATCCTCCAATGGGACGACGGCACCCGCATCGCCTTGGTGACCAAGGTCACCGGCAACACCTGGCGCG
ACGAACCGGGCTATGTGGTGCTGCGTGACTGGCAGGACGCGGGACTGAGCAAGCCCAGCGCCGTGCGATGCTCGCAACTG
CTGCGGCTGCCAAGCAATCTTTTCCTCGACGACGGCCCGACAGGAAGACTTTCGGCATATGATGCCGGCCGCGTCACCTG
GGCGATCAACGAACTCTATCCGGGCCTACTAGCCTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10866.37 Da
• Isoelectric Point: 4.2896

>AT76596 WP_016463214.1 NZ_CP021394:c1150471-1150178 [Bifidobacterium breve]
MAIKEKATISLDAQTKRDGIAILDAMGLNLSTFAEMSLRQLVRDGRLPFMPSVRPSFEKDNEGYPLFKANMDDPRIVTPQ
IRDGAVILPEGWDDDED

Nucleotide

• Length: 294 bp

>AT76596 NZ_CP021394:c1150471-1150178 [Bifidobacterium breve]
ATGGCTATCAAAGAGAAGGCCACGATCAGTCTGGATGCGCAGACCAAGCGTGACGGCATCGCCATACTTGACGCCATGGG
GTTGAACCTGAGCACGTTTGCCGAAATGTCTCTCAGACAATTGGTACGCGACGGGCGTCTGCCATTCATGCCAAGCGTCC
GCCCTTCATTCGAAAAAGACAACGAGGGATATCCCCTGTTCAAAGCGAACATGGATGATCCCCGCATCGTGACGCCCCAG
ATACGTGATGGTGCCGTGATCCTGCCGGAGGGTTGGGACGATGATGAAGACTGA

Structures

Toxin

• AlphaFold DB: A0A0M3T8E7