Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-RelB
Location 1063213..1063768 Replicon chromosome
Accession NZ_CP021394
Organism Bifidobacterium breve strain NRBB56

Toxin (Protein)


Gene name mazF Uniprot ID S2ZXD7
Locus tag NRBB56_RS04640 Protein ID WP_003829109.1
Coordinates 1063213..1063536 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S2ZDC4
Locus tag NRBB56_RS04645 Protein ID WP_003829112.1
Coordinates 1063523..1063768 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NRBB56_RS04605 1058250..1058538 - 289 Protein_857 IS256 family transposase -
NRBB56_RS11030 1058756..1058911 + 156 WP_019728038.1 hypothetical protein -
NRBB56_RS04615 1059009..1060274 - 1266 WP_015438706.1 Fic family protein -
NRBB56_RS04625 1060816..1061181 - 366 WP_025299744.1 YccF domain-containing protein -
NRBB56_RS04630 1061419..1062288 + 870 WP_003829104.1 class I SAM-dependent methyltransferase -
NRBB56_RS04635 1062382..1062996 + 615 WP_003829107.1 cupin domain-containing protein -
NRBB56_RS04640 1063213..1063536 - 324 WP_003829109.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
NRBB56_RS04645 1063523..1063768 - 246 WP_003829112.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
NRBB56_RS04650 1063942..1065198 + 1257 WP_106630395.1 HipA domain-containing protein -
NRBB56_RS04655 1065463..1065654 + 192 WP_106630396.1 XRE family transcriptional regulator -
NRBB56_RS04660 1065838..1067109 - 1272 WP_003829121.1 MFS transporter -
NRBB56_RS04665 1067266..1067928 - 663 WP_019728044.1 TetR/AcrR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 1041071..1074648 33577


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-103)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(5-56)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 11859.67 Da        Isoelectric Point: 5.0771

>T76595 WP_003829109.1 NZ_CP021394:c1063536-1063213 [Bifidobacterium breve]
MKRGEIWTLQADGYASKPRPVVIVQSDAVDRFDSVITCLLTSYDSSDIDTRVRLEPSPENGLNKVSYVMTDKIVTVDRKL
LGYQVGVVDDAAMANIGRQLMRVLGLL

Download         Length: 324 bp

>T76595 NZ_CP021394:c1063536-1063213 [Bifidobacterium breve]
ATGAAACGAGGTGAGATTTGGACGTTGCAGGCCGATGGCTACGCCAGCAAGCCACGGCCGGTTGTCATCGTGCAGAGCGA
TGCCGTCGATCGGTTCGATTCCGTCATCACTTGTCTGCTGACCTCATACGATTCCTCCGACATCGACACGCGGGTCCGAT
TGGAACCAAGTCCGGAGAACGGCCTGAACAAAGTGAGTTACGTGATGACGGACAAGATCGTCACCGTCGACCGCAAACTG
CTGGGATACCAAGTCGGCGTGGTTGATGATGCAGCCATGGCGAATATCGGCCGTCAGCTCATGCGGGTTCTAGGTTTGCT
GTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9339.43 Da        Isoelectric Point: 4.5881

>AT76595 WP_003829112.1 NZ_CP021394:c1063768-1063523 [Bifidobacterium breve]
MSMQIATRVDDEQAALFKETTRQLGTTPADALRMFISAFNDYRGFPYEVRLPRNDVEPFASERDATEYASRLALRMSDET
R

Download         Length: 246 bp

>AT76595 NZ_CP021394:c1063768-1063523 [Bifidobacterium breve]
ATGAGCATGCAGATTGCCACGCGAGTTGATGACGAGCAGGCTGCGTTGTTTAAGGAGACGACCCGACAGCTGGGTACCAC
CCCGGCCGATGCCCTGCGTATGTTCATCAGCGCGTTCAATGATTACCGTGGGTTCCCCTATGAGGTGCGTTTGCCACGAA
ATGACGTGGAGCCGTTTGCGTCCGAGCGGGACGCTACTGAGTATGCCAGTCGTCTCGCCTTGAGGATGTCCGATGAAACG
AGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A087B6Q7

Antitoxin

Source ID Structure
AlphaFold DB A0A087B6Q6

References