Detailed information of TA system TA076593

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/DinJ(antitoxin)
• Location: 1061835..1062471
• Replicon: chromosome
• Accession: NZ_CP021393
• Organism: Bifidobacterium breve strain NRBB52

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A0M3T8E7
• Locus tag: NRBB52_RS04730
• Protein ID: WP_052787173.1
• Coordinates: 1061835..1062191 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: NRBB52_RS04735
• Protein ID: WP_016463214.1
• Coordinates: 1062178..1062471 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NRBB52_RS04700	1056887..1057798	+	912	WP_016463209.1	ABC transporter ATP-binding protein	-
NRBB52_RS04705	1057995..1058621	+	627	WP_003829226.1	30S ribosomal protein S4	-
NRBB52_RS04710	1059096..1059878	-	783	WP_016463211.1	gamma-glutamyl-gamma-aminobutyrate hydrolase family protein	-
NRBB52_RS04715	1060020..1060505	-	486	WP_003829229.1	cytidine deaminase	-
NRBB52_RS04720	1060595..1060951	+	357	WP_003833231.1	SdpI family protein	-
NRBB52_RS04725	1061056..1061658	+	603	WP_016463212.1	transglutaminase family protein	-
NRBB52_RS04730	1061835..1062191	-	357	WP_052787173.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
NRBB52_RS04735	1062178..1062471	-	294	WP_016463214.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
NRBB52_RS04740	1062618..1062788	+	171	Protein_885	helix-turn-helix domain-containing protein	-
NRBB52_RS04745	1062929..1063714	-	786	WP_014483316.1	IS21-like element helper ATPase IstB	-
NRBB52_RS04750	1063717..1065198	-	1482	WP_015438265.1	IS21 family transposase	-
NRBB52_RS04755	1065369..1066215	+	847	Protein_888	IS30 family transposase	-
NRBB52_RS04760	1066336..1066656	-	321	WP_016463217.1	hypothetical protein	-
NRBB52_RS04765	1066675..1067292	-	618	WP_141671914.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1062929..1063714	785

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-110)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (11-53)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13313.23 Da
• Isoelectric Point: 7.9984

>T76593 WP_052787173.1 NZ_CP021393:c1062191-1061835 [Bifidobacterium breve]
MMKTDPHQFEIWWVPFAFPDKPGKAKNRPSVILQWDDGTRIALVTKVTGNTWRDEPGYVVLRDWQDAGLSKPSAVRCSQL
LRLPSNLFLDDGPTGRLSAYDAGRVTWAINELYPGLLA

Nucleotide

• Length: 357 bp

>T76593 NZ_CP021393:c1062191-1061835 [Bifidobacterium breve]
ATGATGAAGACTGATCCGCACCAGTTCGAGATATGGTGGGTGCCGTTCGCTTTTCCCGACAAGCCAGGCAAAGCGAAGAA
CCGTCCCAGCGTGATCCTCCAATGGGACGACGGCACCCGCATCGCCTTGGTGACCAAGGTCACCGGCAACACCTGGCGCG
ACGAACCGGGCTATGTGGTGCTGCGTGACTGGCAGGACGCGGGACTGAGCAAGCCCAGCGCCGTGCGATGCTCGCAACTG
CTGCGGCTGCCAAGCAATCTTTTCCTCGACGACGGCCCGACAGGAAGACTTTCGGCATATGATGCCGGCCGCGTCACCTG
GGCGATCAACGAACTCTATCCGGGCCTACTAGCCTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10866.37 Da
• Isoelectric Point: 4.2896

>AT76593 WP_016463214.1 NZ_CP021393:c1062471-1062178 [Bifidobacterium breve]
MAIKEKATISLDAQTKRDGIAILDAMGLNLSTFAEMSLRQLVRDGRLPFMPSVRPSFEKDNEGYPLFKANMDDPRIVTPQ
IRDGAVILPEGWDDDED

Nucleotide

• Length: 294 bp

>AT76593 NZ_CP021393:c1062471-1062178 [Bifidobacterium breve]
ATGGCTATCAAAGAGAAGGCCACGATCAGTCTGGATGCGCAGACCAAGCGTGACGGCATCGCCATACTTGACGCCATGGG
GTTGAACCTGAGCACGTTTGCCGAAATGTCTCTCAGACAATTGGTACGCGACGGGCGTCTGCCATTCATGCCAAGCGTCC
GCCCTTCATTCGAAAAAGACAACGAGGGATATCCCCTGTTCAAAGCGAACATGGATGATCCCCGCATCGTGACGCCCCAG
ATACGTGATGGTGCCGTGATCCTGCCGGAGGGTTGGGACGATGATGAAGACTGA

Structures

Toxin

• AlphaFold DB: A0A0M3T8E7