Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-RelB
Location 992352..992907 Replicon chromosome
Accession NZ_CP021393
Organism Bifidobacterium breve strain NRBB52

Toxin (Protein)


Gene name mazF Uniprot ID S2ZXD7
Locus tag NRBB52_RS04395 Protein ID WP_003829109.1
Coordinates 992352..992675 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S2ZDC4
Locus tag NRBB52_RS04400 Protein ID WP_003829112.1
Coordinates 992662..992907 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NRBB52_RS04360 987389..987677 - 289 Protein_814 IS256 family transposase -
NRBB52_RS04370 988148..989413 - 1266 WP_015438706.1 Fic family protein -
NRBB52_RS04380 989955..990320 - 366 WP_025299744.1 YccF domain-containing protein -
NRBB52_RS04385 990558..991427 + 870 WP_003829104.1 class I SAM-dependent methyltransferase -
NRBB52_RS04390 991521..992135 + 615 WP_003829107.1 cupin domain-containing protein -
NRBB52_RS04395 992352..992675 - 324 WP_003829109.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
NRBB52_RS04400 992662..992907 - 246 WP_003829112.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
NRBB52_RS04405 993079..994335 + 1257 WP_015438708.1 HipA domain-containing protein -
NRBB52_RS04410 994601..994792 + 192 WP_003829118.1 hypothetical protein -
NRBB52_RS04415 994976..996247 - 1272 WP_025301184.1 MFS transporter -
NRBB52_RS04420 996404..997066 - 663 WP_014483844.1 TetR/AcrR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 982122..1026612 44490


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-103)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(5-56)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 11859.67 Da        Isoelectric Point: 5.0771

>T76592 WP_003829109.1 NZ_CP021393:c992675-992352 [Bifidobacterium breve]
MKRGEIWTLQADGYASKPRPVVIVQSDAVDRFDSVITCLLTSYDSSDIDTRVRLEPSPENGLNKVSYVMTDKIVTVDRKL
LGYQVGVVDDAAMANIGRQLMRVLGLL

Download         Length: 324 bp

>T76592 NZ_CP021393:c992675-992352 [Bifidobacterium breve]
ATGAAACGAGGTGAGATTTGGACGTTGCAGGCCGATGGCTACGCCAGCAAGCCACGGCCGGTTGTCATCGTGCAGAGCGA
TGCCGTCGATCGGTTCGATTCCGTCATCACTTGTCTGCTGACCTCATACGATTCCTCCGACATCGACACGCGGGTCCGAT
TGGAACCAAGTCCGGAGAACGGCCTGAACAAAGTGAGTTACGTGATGACGGACAAGATCGTCACCGTCGACCGCAAACTG
CTGGGATACCAAGTCGGCGTGGTTGATGATGCAGCCATGGCGAATATCGGCCGTCAGCTCATGCGGGTTCTAGGTTTGCT
GTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9339.43 Da        Isoelectric Point: 4.5881

>AT76592 WP_003829112.1 NZ_CP021393:c992907-992662 [Bifidobacterium breve]
MSMQIATRVDDEQAALFKETTRQLGTTPADALRMFISAFNDYRGFPYEVRLPRNDVEPFASERDATEYASRLALRMSDET
R

Download         Length: 246 bp

>AT76592 NZ_CP021393:c992907-992662 [Bifidobacterium breve]
ATGAGCATGCAGATTGCCACGCGAGTTGATGACGAGCAGGCTGCGTTGTTTAAGGAGACGACCCGACAGCTGGGTACCAC
CCCGGCCGATGCCCTGCGTATGTTCATCAGCGCGTTCAATGATTACCGTGGGTTCCCCTATGAGGTGCGTTTGCCACGAA
ATGACGTGGAGCCGTTTGCGTCCGAGCGGGACGCTACTGAGTATGCCAGTCGTCTCGCCTTGAGGATGTCCGATGAAACG
AGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A087B6Q7

Antitoxin

Source ID Structure
AlphaFold DB A0A087B6Q6

References