Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/RelB(antitoxin)
Location 1025961..1026597 Replicon chromosome
Accession NZ_CP021392
Organism Bifidobacterium breve strain NRBB51

Toxin (Protein)


Gene name mazF Uniprot ID D4BP71
Locus tag NRBB51_RS04490 Protein ID WP_003829234.1
Coordinates 1025961..1026317 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID D6PAX1
Locus tag NRBB51_RS04495 Protein ID WP_012577921.1
Coordinates 1026304..1026597 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NRBB51_RS04460 1021149..1022060 + 912 WP_025341717.1 ABC transporter ATP-binding protein -
NRBB51_RS04465 1022257..1022883 + 627 WP_003829226.1 30S ribosomal protein S4 -
NRBB51_RS04470 1023221..1024003 - 783 WP_103619852.1 gamma-glutamyl-gamma-aminobutyrate hydrolase family protein -
NRBB51_RS04475 1024145..1024630 - 486 WP_003829229.1 cytidine deaminase -
NRBB51_RS04480 1024721..1025077 + 357 WP_003833231.1 SdpI family protein -
NRBB51_RS04485 1025182..1025784 + 603 WP_003829233.1 transglutaminase family protein -
NRBB51_RS04490 1025961..1026317 - 357 WP_003829234.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
NRBB51_RS04495 1026304..1026597 - 294 WP_012577921.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
NRBB51_RS04505 1027053..1027727 + 675 WP_015438748.1 histidine phosphatase family protein -
NRBB51_RS04510 1027810..1028214 + 405 WP_003829238.1 DUF948 domain-containing protein -
NRBB51_RS04515 1028214..1028450 + 237 WP_003829239.1 hypothetical protein -
NRBB51_RS04520 1028546..1031224 + 2679 WP_065452338.1 alanine--tRNA ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-110)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(11-53)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 13316.23 Da        Isoelectric Point: 7.9984

>T76590 WP_003829234.1 NZ_CP021392:c1026317-1025961 [Bifidobacterium breve]
MMKTDPHQFEIWWVPFTFPDKPGKAKNRPSVILQWDDGTRIALVTKVTGNTWRDEPGYVVLRDWQDAGLSKPSAVRCSQL
LRLPSSLFLDDGPTGRLSAYDAGRVTWAINELYPGLLA

Download         Length: 357 bp

>T76590 NZ_CP021392:c1026317-1025961 [Bifidobacterium breve]
ATGATGAAGACTGATCCGCACCAGTTCGAGATATGGTGGGTGCCGTTCACTTTTCCCGACAAGCCAGGCAAAGCGAAGAA
CCGTCCCAGCGTGATCCTCCAATGGGACGACGGCACCCGCATCGCCTTGGTGACCAAGGTCACCGGCAACACCTGGCGCG
ACGAACCGGGCTATGTGGTGCTGCGTGACTGGCAGGACGCGGGACTGAGCAAGCCCAGCGCCGTGCGATGCTCGCAACTG
CTGCGGCTGCCAAGCAGTCTTTTCCTCGACGACGGCCCGACAGGAAGACTTTCGGCATATGATGCCGGCCGCGTCACCTG
GGCGATCAACGAACTCTATCCGGGCCTACTAGCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10866.31 Da        Isoelectric Point: 4.2896

>AT76590 WP_012577921.1 NZ_CP021392:c1026597-1026304 [Bifidobacterium breve]
MAIKEKTTISLDAQTKRDGIAILDAMGLNLSTFAEMSLRQLVRDGRLPFTPSVRPSFEKDNEGYPLFKANMDDPRIVTPQ
IRDGAVILPEGWDDDED

Download         Length: 294 bp

>AT76590 NZ_CP021392:c1026597-1026304 [Bifidobacterium breve]
ATGGCTATCAAGGAGAAGACCACGATCAGTCTGGATGCGCAGACCAAGCGTGACGGCATCGCCATACTTGACGCCATGGG
GTTGAACCTGAGCACGTTTGCCGAAATGTCTCTCAGACAATTGGTACGCGACGGGCGTCTGCCATTCACGCCAAGCGTCC
GCCCTTCATTCGAAAAAGACAACGAGGGATATCCCCTGTTCAAAGCGAACATGGATGATCCCCGCATCGTGACGCCCCAG
ATACGTGATGGTGCCGTGATCCTGCCGGAGGGTTGGGACGATGATGAAGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0L7B6G7

Antitoxin

Source ID Structure
AlphaFold DB A0A1S2VTZ6

References