Detailed information of TA system TA076588

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafQ-RelB
• Location: 1390882..1391448
• Replicon: chromosome
• Accession: NZ_CP021391
• Organism: Bifidobacterium breve strain NRBB50

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A0L7AXF3
• Locus tag: NRBB50_RS06450
• Protein ID: WP_025262990.1
• Coordinates: 1391155..1391448 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: NRBB50_RS06445
• Protein ID: WP_106647936.1
• Coordinates: 1390882..1391151 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NRBB50_RS06415	1386374..1387447	-	1074	WP_052828322.1	histidine kinase	-
NRBB50_RS06420	1387513..1388208	-	696	WP_003829410.1	response regulator transcription factor	-
NRBB50_RS06425	1388334..1388849	+	516	WP_013140733.1	hypothetical protein	-
NRBB50_RS06430	1388846..1389736	+	891	WP_007056280.1	hypothetical protein	-
NRBB50_RS06435	1389733..1390413	+	681	WP_003829413.1	ABC transporter ATP-binding protein	-
NRBB50_RS06440	1390473..1390697	+	225	Protein_1200	ABC transporter permease	-
NRBB50_RS06445	1390882..1391151	+	270	WP_106647936.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
NRBB50_RS06450	1391155..1391448	+	294	WP_025262990.1	type II toxin-antitoxin system YafQ family toxin	Toxin
NRBB50_RS06460	1392117..1392668	+	552	WP_012577735.1	RNA polymerase sigma factor	-
NRBB50_RS06465	1392702..1393529	+	828	WP_071474967.1	hypothetical protein	-
NRBB50_RS06470	1393661..1394287	+	627	WP_012577737.1	hypothetical protein	-
NRBB50_RS06475	1394284..1395066	+	783	WP_077386957.1	murein hydrolase transporter LrgB	-
NRBB50_RS06480	1395119..1395994	+	876	WP_077386959.1	ABC transporter ATP-binding protein	-
NRBB50_RS06485	1396049..1396447	+	399	WP_015438839.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1380701..1406211	25510

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-95)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (3-88)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11190.93 Da
• Isoelectric Point: 9.7881

>T76588 WP_025262990.1 NZ_CP021391:1391155-1391448 [Bifidobacterium breve]
MLKPDYTAAFQRDIKKLKRKHTDLRPLKEVIRLVLEDTADSKEALRRRHRAHTLTGDLNGVLECHIGNAGDWLLLWIRDD
GTAMFMRTGSHDELLGK

Nucleotide

• Length: 294 bp

>T76588 NZ_CP021391:1391155-1391448 [Bifidobacterium breve]
ATGCTCAAACCGGATTACACAGCGGCATTCCAGCGAGATATCAAGAAACTCAAACGCAAGCACACTGATTTACGCCCGCT
GAAAGAAGTCATTCGTCTGGTTCTTGAGGACACGGCGGACTCCAAGGAAGCATTGAGGCGTAGGCATCGCGCCCACACAC
TTACCGGTGATTTAAACGGCGTACTGGAATGCCACATCGGCAATGCCGGCGACTGGCTGCTTCTATGGATTCGCGATGAT
GGTACCGCCATGTTCATGCGAACCGGCAGCCATGACGAACTGTTGGGCAAATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9876.17 Da
• Isoelectric Point: 4.7872

>AT76588 WP_106647936.1 NZ_CP021391:1390882-1391151 [Bifidobacterium breve]
VSMASVTVRVDADTKQKAANIAEDLGLDLSSVTRAFYKQIVRERRIPLNLSYTTVPPETMEALDESKNRLADGKPRFNSA
DEMFDSLGI

Nucleotide

• Length: 270 bp

>AT76588 NZ_CP021391:1390882-1391151 [Bifidobacterium breve]
GTGAGCATGGCATCCGTCACAGTCCGCGTGGATGCGGACACCAAGCAGAAAGCCGCAAACATAGCCGAGGATCTCGGTCT
GGACTTGTCTTCGGTCACCCGTGCCTTCTACAAGCAGATTGTCCGGGAGAGGCGCATTCCTCTAAACCTTTCCTATACGA
CCGTGCCGCCTGAAACCATGGAGGCCCTTGACGAGTCGAAAAACAGGCTCGCCGACGGCAAACCTCGTTTCAACAGTGCC
GATGAGATGTTCGATTCGTTGGGAATCTGA

Structures

Toxin

• AlphaFold DB: A0A0L7AXF3