Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/RelB(antitoxin)
Location 1191997..1192633 Replicon chromosome
Accession NZ_CP021391
Organism Bifidobacterium breve strain NRBB50

Toxin (Protein)


Gene name mazF Uniprot ID A0A133L4T2
Locus tag NRBB50_RS05440 Protein ID WP_021649636.1
Coordinates 1191997..1192353 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID D6PAX1
Locus tag NRBB50_RS05445 Protein ID WP_012577921.1
Coordinates 1192340..1192633 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NRBB50_RS05410 1187055..1187963 + 909 WP_021649641.1 ABC transporter ATP-binding protein -
NRBB50_RS05415 1188160..1188786 + 627 WP_003829226.1 30S ribosomal protein S4 -
NRBB50_RS05420 1189262..1190044 - 783 WP_021649638.1 gamma-glutamyl-gamma-aminobutyrate hydrolase family protein -
NRBB50_RS05425 1190182..1190667 - 486 WP_021649637.1 cytidine deaminase -
NRBB50_RS05430 1190757..1191113 + 357 WP_012577924.1 SdpI family protein -
NRBB50_RS05435 1191218..1191820 + 603 WP_003829233.1 transglutaminase family protein -
NRBB50_RS05440 1191997..1192353 - 357 WP_021649636.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
NRBB50_RS05445 1192340..1192633 - 294 WP_012577921.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
NRBB50_RS05450 1192885..1193559 + 675 WP_015438748.1 histidine phosphatase family protein -
NRBB50_RS05455 1193642..1194025 + 384 WP_016463075.1 DUF948 domain-containing protein -
NRBB50_RS05460 1194025..1194261 + 237 WP_003829239.1 hypothetical protein -
NRBB50_RS05465 1194357..1197035 + 2679 WP_021649635.1 alanine--tRNA ligase -
NRBB50_RS05470 1197044..1197496 + 453 WP_003829241.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(7-110)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(11-53)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 13332.27 Da        Isoelectric Point: 8.9603

>T76587 WP_021649636.1 NZ_CP021391:c1192353-1191997 [Bifidobacterium breve]
MMKTDPRQFEIWWVPFAFPDKPGKAKNRPSVILQWDDGTRIALVTKVTGNTWRDEPGYVVLRDWQDAGLSKPSAVRCSQL
LRLPSNLFLDDGPTGRLSAYDAGRVTWAINELYPGLLA

Download         Length: 357 bp

>T76587 NZ_CP021391:c1192353-1191997 [Bifidobacterium breve]
ATGATGAAGACTGATCCGCGCCAGTTCGAGATATGGTGGGTGCCGTTCGCTTTTCCCGACAAGCCAGGCAAAGCGAAGAA
CCGTCCCAGCGTGATCCTCCAATGGGACGACGGCACCCGCATCGCCTTGGTGACCAAGGTCACCGGCAACACCTGGCGCG
ACGAACCGGGCTATGTGGTGCTGCGTGACTGGCAGGACGCGGGACTGAGCAAGCCCAGCGCCGTGCGATGCTCGCAACTG
CTGCGGCTGCCAAGCAATCTTTTCCTCGACGACGGCCCGACAGGAAGACTTTCGGCATATGATGCCGGCCGCGTCACCTG
GGCGATCAACGAACTCTATCCGGGCCTACTAGCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10866.31 Da        Isoelectric Point: 4.2896

>AT76587 WP_012577921.1 NZ_CP021391:c1192633-1192340 [Bifidobacterium breve]
MAIKEKTTISLDAQTKRDGIAILDAMGLNLSTFAEMSLRQLVRDGRLPFTPSVRPSFEKDNEGYPLFKANMDDPRIVTPQ
IRDGAVILPEGWDDDED

Download         Length: 294 bp

>AT76587 NZ_CP021391:c1192633-1192340 [Bifidobacterium breve]
ATGGCTATCAAGGAGAAGACCACGATCAGTCTGGATGCGCAGACCAAGCGTGACGGCATCGCCATACTTGACGCCATGGG
GTTGAACCTGAGCACGTTTGCCGAAATGTCTCTCAGACAATTGGTACGCGACGGGCGTCTGCCATTCACGCCAAGCGTCC
GCCCTTCATTCGAAAAAGACAACGAGGGATATCCCCTGTTCAAAGCGAACATGGATGATCCCCGCATCGTGACGCCCCAG
ATACGTGATGGTGCCGTGATCCTGCCGGAGGGTTGGGACGATGATGAAGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A133L4T2

Antitoxin

Source ID Structure
AlphaFold DB A0A1S2VTZ6

References