Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-RelB
Location 1129112..1129667 Replicon chromosome
Accession NZ_CP021391
Organism Bifidobacterium breve strain NRBB50

Toxin (Protein)


Gene name mazF Uniprot ID B7GSS5
Locus tag NRBB50_RS05130 Protein ID WP_003833460.1
Coordinates 1129112..1129435 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S2ZDC4
Locus tag NRBB50_RS05135 Protein ID WP_003829112.1
Coordinates 1129422..1129667 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NRBB50_RS05095 1124149..1124437 - 289 Protein_951 IS256 family transposase -
NRBB50_RS05105 1124908..1126173 - 1266 WP_025262925.1 Fic family protein -
NRBB50_RS05115 1126715..1127080 - 366 WP_013140585.1 YccF domain-containing protein -
NRBB50_RS05120 1127318..1128187 + 870 WP_003829104.1 class I SAM-dependent methyltransferase -
NRBB50_RS05125 1128281..1128895 + 615 WP_003829107.1 cupin domain-containing protein -
NRBB50_RS05130 1129112..1129435 - 324 WP_003833460.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
NRBB50_RS05135 1129422..1129667 - 246 WP_003829112.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
NRBB50_RS05140 1129841..1131097 + 1257 WP_003829116.1 HipA domain-containing protein -
NRBB50_RS05145 1131362..1131553 + 192 WP_003829118.1 hypothetical protein -
NRBB50_RS05150 1131737..1133008 - 1272 WP_052790209.1 MFS transporter -
NRBB50_RS05155 1133165..1133827 - 663 WP_052790211.1 TetR/AcrR family transcriptional regulator -
NRBB50_RS11215 1134289..1134474 + 186 WP_003808830.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1119784..1156956 37172


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-106)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(5-56)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 11829.64 Da        Isoelectric Point: 6.2932

>T76586 WP_003833460.1 NZ_CP021391:c1129435-1129112 [Bifidobacterium breve]
MKRGEIRTLQADGYASKPRPVVIVQSDAVDRFDSVITCLLTSYDSSDIDTRVRLEPSPENGLNKVSYVMTDKIVTVDRKL
LGYQVGVVDDAAMANIGRQLMRVLGLL

Download         Length: 324 bp

>T76586 NZ_CP021391:c1129435-1129112 [Bifidobacterium breve]
ATGAAACGAGGTGAGATTCGGACGTTGCAGGCCGATGGCTACGCCAGCAAGCCACGGCCGGTTGTCATCGTGCAGAGCGA
TGCCGTCGATCGGTTCGATTCCGTCATCACTTGTCTGCTGACCTCATACGATTCCTCCGACATCGACACGCGGGTCCGAT
TGGAACCAAGTCCGGAGAACGGCCTGAACAAAGTGAGTTACGTGATGACGGACAAGATCGTCACCGTCGACCGCAAACTG
CTGGGATACCAAGTCGGCGTGGTTGATGATGCAGCCATGGCGAATATCGGCCGTCAGCTCATGCGGGTTCTAGGTTTGCT
GTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9339.43 Da        Isoelectric Point: 4.5881

>AT76586 WP_003829112.1 NZ_CP021391:c1129667-1129422 [Bifidobacterium breve]
MSMQIATRVDDEQAALFKETTRQLGTTPADALRMFISAFNDYRGFPYEVRLPRNDVEPFASERDATEYASRLALRMSDET
R

Download         Length: 246 bp

>AT76586 NZ_CP021391:c1129667-1129422 [Bifidobacterium breve]
ATGAGCATGCAGATTGCCACGCGAGTTGATGACGAGCAGGCTGCGTTGTTTAAGGAGACGACCCGACAGCTGGGTACCAC
CCCGGCCGATGCCCTGCGTATGTTCATCAGCGCGTTCAATGATTACCGTGGGTTCCCCTATGAGGTGCGTTTGCCACGAA
ATGACGTGGAGCCGTTTGCGTCCGAGCGGGACGCTACTGAGTATGCCAGTCGTCTCGCCTTGAGGATGTCCGATGAAACG
AGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4R0SL31

Antitoxin

Source ID Structure
AlphaFold DB A0A087B6Q6

References