Detailed information of TA system TA076583

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/DinJ(antitoxin)
• Location: 1104298..1104934
• Replicon: chromosome
• Accession: NZ_CP021390
• Organism: Bifidobacterium breve strain DRBB26

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A0L0LSR6
• Locus tag: DRBB26_RS04850
• Protein ID: WP_050824947.1
• Coordinates: 1104298..1104654 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: DRBB26_RS04855
• Protein ID: WP_016463214.1
• Coordinates: 1104641..1104934 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DRBB26_RS04820	1099490..1100401	+	912	WP_106628256.1	ABC transporter ATP-binding protein	-
DRBB26_RS04825	1100598..1101224	+	627	WP_003829226.1	30S ribosomal protein S4	-
DRBB26_RS04830	1101562..1102344	-	783	WP_106628257.1	gamma-glutamyl-gamma-aminobutyrate hydrolase family protein	-
DRBB26_RS04835	1102482..1102967	-	486	WP_014483808.1	cytidine deaminase	-
DRBB26_RS04840	1103058..1103414	+	357	WP_106628258.1	SdpI family protein	-
DRBB26_RS04845	1103519..1104121	+	603	WP_003829233.1	transglutaminase family protein	-
DRBB26_RS04850	1104298..1104654	-	357	WP_050824947.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
DRBB26_RS04855	1104641..1104934	-	294	WP_016463214.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
DRBB26_RS04865	1105350..1106024	+	675	WP_015438748.1	histidine phosphatase family protein	-
DRBB26_RS04870	1106107..1106490	+	384	WP_016463075.1	DUF948 domain-containing protein	-
DRBB26_RS04875	1106490..1106726	+	237	WP_003829239.1	hypothetical protein	-
DRBB26_RS04880	1106822..1109500	+	2679	WP_003833243.1	alanine--tRNA ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-110)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (11-53)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13283.20 Da
• Isoelectric Point: 7.9984

>T76583 WP_050824947.1 NZ_CP021390:c1104654-1104298 [Bifidobacterium breve]
MMKTDPHQFEIWWVPFAFPDKPGKAKNRPSVILQWDDGTRIALVTKVTGNTWRDEPGYVVLRDWQDAGLSKPGAVRCSQL
LRLPSNLFLDDGPTGRLSAYDAGRVTWAINELYPGLLA

Nucleotide

• Length: 357 bp

>T76583 NZ_CP021390:c1104654-1104298 [Bifidobacterium breve]
ATGATGAAGACTGATCCGCACCAGTTCGAGATATGGTGGGTGCCGTTCGCTTTTCCCGACAAGCCAGGCAAAGCGAAGAA
CCGTCCCAGCGTGATCCTCCAATGGGACGACGGCACCCGCATCGCCTTGGTGACCAAGGTCACCGGCAACACCTGGCGCG
ACGAACCGGGCTATGTGGTGCTGCGTGACTGGCAGGACGCGGGACTGAGCAAGCCCGGCGCCGTGCGATGCTCGCAACTG
CTGCGGCTGCCAAGCAATCTTTTCCTCGACGACGGCCCGACAGGAAGACTTTCGGCATATGATGCCGGCCGCGTCACCTG
GGCGATCAACGAACTCTATCCGGGCCTACTAGCCTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10866.37 Da
• Isoelectric Point: 4.2896

>AT76583 WP_016463214.1 NZ_CP021390:c1104934-1104641 [Bifidobacterium breve]
MAIKEKATISLDAQTKRDGIAILDAMGLNLSTFAEMSLRQLVRDGRLPFMPSVRPSFEKDNEGYPLFKANMDDPRIVTPQ
IRDGAVILPEGWDDDED

Nucleotide

• Length: 294 bp

>AT76583 NZ_CP021390:c1104934-1104641 [Bifidobacterium breve]
ATGGCTATCAAAGAGAAGGCCACGATCAGTCTGGATGCGCAGACCAAGCGTGACGGCATCGCCATACTTGACGCCATGGG
GTTGAACCTGAGCACGTTTGCCGAAATGTCTCTCAGACAATTGGTACGCGACGGGCGTCTGCCATTCATGCCAAGCGTCC
GCCCTTCATTCGAAAAAGACAACGAGGGATATCCCCTGTTCAAAGCGAACATGGATGATCCCCGCATCGTGACGCCCCAG
ATACGTGATGGTGCCGTGATCCTGCCGGAGGGTTGGGACGATGATGAAGACTGA

Structures

Toxin

• AlphaFold DB: A0A0L0LSR6