Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-RelB
Location 1052680..1053235 Replicon chromosome
Accession NZ_CP021390
Organism Bifidobacterium breve strain DRBB26

Toxin (Protein)


Gene name mazF Uniprot ID S2ZXD7
Locus tag DRBB26_RS04600 Protein ID WP_003829109.1
Coordinates 1052680..1053003 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S2ZDC4
Locus tag DRBB26_RS04605 Protein ID WP_003829112.1
Coordinates 1052990..1053235 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DRBB26_RS04575 1048476..1049741 - 1266 WP_015438706.1 Fic family protein -
DRBB26_RS04585 1050283..1050648 - 366 WP_025299744.1 YccF domain-containing protein -
DRBB26_RS04590 1050886..1051755 + 870 WP_106628243.1 class I SAM-dependent methyltransferase -
DRBB26_RS04595 1051849..1052463 + 615 WP_003829107.1 cupin domain-containing protein -
DRBB26_RS04600 1052680..1053003 - 324 WP_003829109.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
DRBB26_RS04605 1052990..1053235 - 246 WP_003829112.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
DRBB26_RS04610 1053407..1054663 + 1257 WP_016463167.1 HipA domain-containing protein -
DRBB26_RS04615 1054928..1055119 + 192 WP_003829118.1 hypothetical protein -
DRBB26_RS04620 1055303..1056574 - 1272 WP_052790209.1 MFS transporter -
DRBB26_RS04625 1056731..1057393 - 663 WP_106628244.1 TetR/AcrR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-103)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(5-56)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 11859.67 Da        Isoelectric Point: 5.0771

>T76582 WP_003829109.1 NZ_CP021390:c1053003-1052680 [Bifidobacterium breve]
MKRGEIWTLQADGYASKPRPVVIVQSDAVDRFDSVITCLLTSYDSSDIDTRVRLEPSPENGLNKVSYVMTDKIVTVDRKL
LGYQVGVVDDAAMANIGRQLMRVLGLL

Download         Length: 324 bp

>T76582 NZ_CP021390:c1053003-1052680 [Bifidobacterium breve]
ATGAAACGAGGTGAGATTTGGACGTTGCAGGCCGATGGCTACGCCAGCAAGCCACGGCCGGTTGTCATCGTGCAGAGCGA
TGCCGTCGATCGGTTCGATTCCGTCATCACTTGTCTGCTGACCTCATACGATTCCTCCGACATCGACACGCGGGTCCGAT
TGGAACCAAGTCCGGAGAACGGCCTGAACAAAGTGAGTTACGTGATGACGGACAAGATCGTCACCGTCGACCGCAAACTG
CTGGGATACCAAGTCGGCGTGGTTGATGATGCAGCCATGGCGAATATCGGCCGTCAGCTCATGCGGGTTCTAGGTTTGCT
GTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9339.43 Da        Isoelectric Point: 4.5881

>AT76582 WP_003829112.1 NZ_CP021390:c1053235-1052990 [Bifidobacterium breve]
MSMQIATRVDDEQAALFKETTRQLGTTPADALRMFISAFNDYRGFPYEVRLPRNDVEPFASERDATEYASRLALRMSDET
R

Download         Length: 246 bp

>AT76582 NZ_CP021390:c1053235-1052990 [Bifidobacterium breve]
ATGAGCATGCAGATTGCCACGCGAGTTGATGACGAGCAGGCTGCGTTGTTTAAGGAGACGACCCGACAGCTGGGTACCAC
CCCGGCCGATGCCCTGCGTATGTTCATCAGCGCGTTCAATGATTACCGTGGGTTCCCCTATGAGGTGCGTTTGCCACGAA
ATGACGTGGAGCCGTTTGCGTCCGAGCGGGACGCTACTGAGTATGCCAGTCGTCTCGCCTTGAGGATGTCCGATGAAACG
AGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A087B6Q7

Antitoxin

Source ID Structure
AlphaFold DB A0A087B6Q6

References