Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/RelB(antitoxin)
Location 1053795..1054431 Replicon chromosome
Accession NZ_CP021389
Organism Bifidobacterium breve strain NRBB57

Toxin (Protein)


Gene name mazF Uniprot ID D4BP71
Locus tag NRBB57_RS04640 Protein ID WP_003829234.1
Coordinates 1053795..1054151 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID D6PAX1
Locus tag NRBB57_RS04645 Protein ID WP_012577921.1
Coordinates 1054138..1054431 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NRBB57_RS04610 1048990..1049898 + 909 WP_106620801.1 ABC transporter ATP-binding protein -
NRBB57_RS04615 1050095..1050721 + 627 WP_003829226.1 30S ribosomal protein S4 -
NRBB57_RS04620 1051058..1051840 - 783 WP_106620802.1 gamma-glutamyl-gamma-aminobutyrate hydrolase family protein -
NRBB57_RS04625 1051981..1052466 - 486 WP_003829229.1 cytidine deaminase -
NRBB57_RS04630 1052555..1052911 + 357 WP_003833231.1 SdpI family protein -
NRBB57_RS04635 1053016..1053618 + 603 WP_106620803.1 transglutaminase family protein -
NRBB57_RS04640 1053795..1054151 - 357 WP_003829234.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
NRBB57_RS04645 1054138..1054431 - 294 WP_012577921.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
NRBB57_RS04650 1054582..1054746 - 165 Protein_873 IS30 family transposase -
NRBB57_RS04655 1054846..1055520 + 675 WP_015438748.1 histidine phosphatase family protein -
NRBB57_RS04660 1055603..1056007 + 405 WP_014483805.1 DUF948 domain-containing protein -
NRBB57_RS04665 1056007..1056243 + 237 WP_003829239.1 hypothetical protein -
NRBB57_RS04670 1056339..1059017 + 2679 WP_014483804.1 alanine--tRNA ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-110)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(11-53)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 13316.23 Da        Isoelectric Point: 7.9984

>T76580 WP_003829234.1 NZ_CP021389:c1054151-1053795 [Bifidobacterium breve]
MMKTDPHQFEIWWVPFTFPDKPGKAKNRPSVILQWDDGTRIALVTKVTGNTWRDEPGYVVLRDWQDAGLSKPSAVRCSQL
LRLPSSLFLDDGPTGRLSAYDAGRVTWAINELYPGLLA

Download         Length: 357 bp

>T76580 NZ_CP021389:c1054151-1053795 [Bifidobacterium breve]
ATGATGAAGACTGATCCGCACCAGTTCGAGATATGGTGGGTGCCGTTCACTTTTCCCGACAAGCCAGGCAAAGCGAAGAA
CCGTCCCAGCGTGATCCTCCAATGGGACGACGGCACCCGCATCGCCTTGGTGACCAAGGTCACCGGCAACACCTGGCGCG
ACGAACCGGGCTATGTGGTGCTGCGTGACTGGCAGGACGCGGGACTGAGCAAGCCCAGCGCCGTGCGATGCTCGCAACTG
CTGCGGCTGCCAAGCAGTCTTTTCCTCGACGACGGCCCGACAGGAAGACTTTCGGCATATGATGCCGGCCGCGTCACCTG
GGCGATCAACGAACTCTATCCGGGCCTACTAGCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10866.31 Da        Isoelectric Point: 4.2896

>AT76580 WP_012577921.1 NZ_CP021389:c1054431-1054138 [Bifidobacterium breve]
MAIKEKTTISLDAQTKRDGIAILDAMGLNLSTFAEMSLRQLVRDGRLPFTPSVRPSFEKDNEGYPLFKANMDDPRIVTPQ
IRDGAVILPEGWDDDED

Download         Length: 294 bp

>AT76580 NZ_CP021389:c1054431-1054138 [Bifidobacterium breve]
ATGGCTATCAAGGAGAAGACCACGATCAGTCTGGATGCGCAGACCAAGCGTGACGGCATCGCCATACTTGACGCCATGGG
GTTGAACCTGAGCACGTTTGCCGAAATGTCTCTCAGACAATTGGTACGCGACGGGCGTCTGCCATTCACGCCAAGCGTCC
GCCCTTCATTCGAAAAAGACAACGAGGGATATCCCCTGTTCAAAGCGAACATGGATGATCCCCGCATCGTGACGCCCCAG
ATACGTGATGGTGCCGTGATCCTGCCGGAGGGTTGGGACGATGATGAAGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0L7B6G7

Antitoxin

Source ID Structure
AlphaFold DB A0A1S2VTZ6

References