Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/RelB(antitoxin)
Location 1062510..1063146 Replicon chromosome
Accession NZ_CP021388
Organism Bifidobacterium breve strain NRBB11

Toxin (Protein)


Gene name mazF Uniprot ID A0A2K9BIJ9
Locus tag NRBB11_RS04655 Protein ID WP_032739732.1
Coordinates 1062510..1062866 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A3D8UDK8
Locus tag NRBB11_RS04660 Protein ID WP_019728072.1
Coordinates 1062853..1063146 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NRBB11_RS04625 1057569..1058477 + 909 WP_003833228.1 ABC transporter ATP-binding protein -
NRBB11_RS04630 1058674..1059300 + 627 WP_003829226.1 30S ribosomal protein S4 -
NRBB11_RS04635 1059775..1060557 - 783 WP_003833230.1 gamma-glutamyl-gamma-aminobutyrate hydrolase family protein -
NRBB11_RS04640 1060695..1061179 - 485 Protein_862 cytidine deaminase -
NRBB11_RS04645 1061270..1061626 + 357 WP_003833231.1 SdpI family protein -
NRBB11_RS04650 1061731..1062333 + 603 WP_003833233.1 transglutaminase family protein -
NRBB11_RS04655 1062510..1062866 - 357 WP_032739732.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
NRBB11_RS04660 1062853..1063146 - 294 WP_019728072.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
NRBB11_RS04665 1063398..1064072 + 675 WP_015438748.1 histidine phosphatase family protein -
NRBB11_RS04670 1064155..1064538 + 384 WP_003833240.1 DUF948 domain-containing protein -
NRBB11_RS04675 1064538..1064774 + 237 WP_003829239.1 hypothetical protein -
NRBB11_RS04680 1064870..1067548 + 2679 WP_003833243.1 alanine--tRNA ligase -
NRBB11_RS04685 1067557..1068009 + 453 WP_003833245.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(7-111)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(11-53)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 13244.21 Da        Isoelectric Point: 9.5969

>T76578 WP_032739732.1 NZ_CP021388:c1062866-1062510 [Bifidobacterium breve]
MMKTDPRQFEIWWVPFAFPDKPGKAKNRPSVILQWDGGTRIALVTKVTGNTWRDEPGYVVLRDWQDAGLSKPSAVRCSQL
LRLPSNLFLDDGPTGRLSAYDAGRVAWAINELYPGLLA

Download         Length: 357 bp

>T76578 NZ_CP021388:c1062866-1062510 [Bifidobacterium breve]
ATGATGAAGACTGATCCGCGCCAGTTCGAGATATGGTGGGTGCCGTTCGCTTTTCCTGACAAGCCAGGCAAAGCGAAGAA
CCGTCCCAGCGTGATCCTCCAATGGGACGGCGGCACCCGCATCGCCTTGGTGACCAAGGTCACCGGCAACACCTGGCGCG
ACGAACCGGGCTATGTGGTGCTGCGTGACTGGCAGGACGCGGGACTGAGCAAGCCCAGCGCCGTGCGATGCTCGCAACTG
CTGCGGCTGCCAAGCAATCTTTTCCTCGACGACGGCCCGACAGGAAGACTTTCGGCATATGATGCCGGCCGCGTCGCCTG
GGCGATCAACGAACTCTATCCGGGCCTACTAGCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10894.32 Da        Isoelectric Point: 4.2189

>AT76578 WP_019728072.1 NZ_CP021388:c1063146-1062853 [Bifidobacterium breve]
MAIKEKATISLDAQTKRDGIAILDAMGLNLSTFAEMSLRQLVRDGRLPFTPSVRPSFEKDNEGYPLFKANMDDPRIVTPQ
IRDGAVILPEDWDDDED

Download         Length: 294 bp

>AT76578 NZ_CP021388:c1063146-1062853 [Bifidobacterium breve]
ATGGCTATCAAGGAGAAGGCCACGATCAGTCTGGATGCGCAGACCAAGCGTGACGGCATCGCCATACTTGACGCCATGGG
GTTGAACCTGAGCACGTTTGCCGAAATGTCTCTCAGACAATTGGTACGCGACGGGCGTCTGCCATTCACGCCAAGCGTCC
GCCCTTCATTCGAAAAAGACAACGAGGGATATCCCCTGTTCAAAGCGAACATGGATGATCCCCGCATCGTGACGCCCCAG
ATACGTGATGGTGCCGTGATCCTGCCGGAGGATTGGGACGATGATGAAGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2K9BIJ9

Antitoxin

Source ID Structure
AlphaFold DB A0A3D8UDK8

References