Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/RelB(antitoxin)
Location 1043690..1044326 Replicon chromosome
Accession NZ_CP021386
Organism Bifidobacterium breve strain NRBB04

Toxin (Protein)


Gene name mazF Uniprot ID D4BP71
Locus tag NRBB04_RS04555 Protein ID WP_003829234.1
Coordinates 1043690..1044046 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID D6PAX1
Locus tag NRBB04_RS04560 Protein ID WP_012577921.1
Coordinates 1044033..1044326 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NRBB04_RS04525 1038879..1039790 + 912 WP_003829224.1 ABC transporter ATP-binding protein -
NRBB04_RS04530 1039987..1040613 + 627 WP_003829226.1 30S ribosomal protein S4 -
NRBB04_RS04535 1040950..1041732 - 783 WP_015438747.1 gamma-glutamyl-gamma-aminobutyrate hydrolase family protein -
NRBB04_RS04540 1041875..1042360 - 486 WP_003829229.1 cytidine deaminase -
NRBB04_RS04545 1042450..1042806 + 357 WP_003829232.1 SdpI family protein -
NRBB04_RS04550 1042911..1043513 + 603 WP_003829233.1 transglutaminase family protein -
NRBB04_RS04555 1043690..1044046 - 357 WP_003829234.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
NRBB04_RS04560 1044033..1044326 - 294 WP_012577921.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
NRBB04_RS04565 1044477..1044641 - 165 Protein_848 IS30 family transposase -
NRBB04_RS04570 1044741..1045415 + 675 WP_025262941.1 histidine phosphatase family protein -
NRBB04_RS04575 1045498..1045902 + 405 WP_003829238.1 DUF948 domain-containing protein -
NRBB04_RS04580 1045902..1046138 + 237 WP_003829239.1 hypothetical protein -
NRBB04_RS04585 1046234..1048912 + 2679 WP_003829240.1 alanine--tRNA ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-110)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(11-53)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 13316.23 Da        Isoelectric Point: 7.9984

>T76574 WP_003829234.1 NZ_CP021386:c1044046-1043690 [Bifidobacterium breve]
MMKTDPHQFEIWWVPFTFPDKPGKAKNRPSVILQWDDGTRIALVTKVTGNTWRDEPGYVVLRDWQDAGLSKPSAVRCSQL
LRLPSSLFLDDGPTGRLSAYDAGRVTWAINELYPGLLA

Download         Length: 357 bp

>T76574 NZ_CP021386:c1044046-1043690 [Bifidobacterium breve]
ATGATGAAGACTGATCCGCACCAGTTCGAGATATGGTGGGTGCCGTTCACTTTTCCCGACAAGCCAGGCAAAGCGAAGAA
CCGTCCCAGCGTGATCCTCCAATGGGACGACGGCACCCGCATCGCCTTGGTGACCAAGGTCACCGGCAACACCTGGCGCG
ACGAACCGGGCTATGTGGTGCTGCGTGACTGGCAGGACGCGGGACTGAGCAAGCCCAGCGCCGTGCGATGCTCGCAACTG
CTGCGGCTGCCAAGCAGTCTTTTCCTCGACGACGGCCCGACAGGAAGACTTTCGGCATATGATGCCGGCCGCGTCACCTG
GGCGATCAACGAACTCTATCCGGGCCTACTAGCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10866.31 Da        Isoelectric Point: 4.2896

>AT76574 WP_012577921.1 NZ_CP021386:c1044326-1044033 [Bifidobacterium breve]
MAIKEKTTISLDAQTKRDGIAILDAMGLNLSTFAEMSLRQLVRDGRLPFTPSVRPSFEKDNEGYPLFKANMDDPRIVTPQ
IRDGAVILPEGWDDDED

Download         Length: 294 bp

>AT76574 NZ_CP021386:c1044326-1044033 [Bifidobacterium breve]
ATGGCTATCAAGGAGAAGACCACGATCAGTCTGGATGCGCAGACCAAGCGTGACGGCATCGCCATACTTGACGCCATGGG
GTTGAACCTGAGCACGTTTGCCGAAATGTCTCTCAGACAATTGGTACGCGACGGGCGTCTGCCATTCACGCCAAGCGTCC
GCCCTTCATTCGAAAAAGACAACGAGGGATATCCCCTGTTCAAAGCGAACATGGATGATCCCCGCATCGTGACGCCCCAG
ATACGTGATGGTGCCGTGATCCTGCCGGAGGGTTGGGACGATGATGAAGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0L7B6G7

Antitoxin

Source ID Structure
AlphaFold DB A0A1S2VTZ6

References