Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-RelB
Location 984375..984930 Replicon chromosome
Accession NZ_CP021386
Organism Bifidobacterium breve strain NRBB04

Toxin (Protein)


Gene name mazF Uniprot ID B7GSS5
Locus tag NRBB04_RS04270 Protein ID WP_003833460.1
Coordinates 984375..984698 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S2ZDC4
Locus tag NRBB04_RS04275 Protein ID WP_003829112.1
Coordinates 984685..984930 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NRBB04_RS04235 979412..979700 - 289 Protein_786 IS256 family transposase -
NRBB04_RS04245 980171..981436 - 1266 WP_025262925.1 Fic family protein -
NRBB04_RS04255 981978..982343 - 366 WP_013140585.1 YccF domain-containing protein -
NRBB04_RS04260 982581..983450 + 870 WP_003829104.1 class I SAM-dependent methyltransferase -
NRBB04_RS04265 983544..984158 + 615 WP_003829107.1 cupin domain-containing protein -
NRBB04_RS04270 984375..984698 - 324 WP_003833460.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
NRBB04_RS04275 984685..984930 - 246 WP_003829112.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
NRBB04_RS04280 985104..986360 + 1257 WP_003829116.1 HipA domain-containing protein -
NRBB04_RS04285 986625..986816 + 192 WP_003829118.1 hypothetical protein -
NRBB04_RS04290 987000..988271 - 1272 WP_003829121.1 MFS transporter -
NRBB04_RS04295 988428..989090 - 663 WP_025262926.1 TetR/AcrR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 974145..1022430 48285


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-106)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(5-56)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 11829.64 Da        Isoelectric Point: 6.2932

>T76573 WP_003833460.1 NZ_CP021386:c984698-984375 [Bifidobacterium breve]
MKRGEIRTLQADGYASKPRPVVIVQSDAVDRFDSVITCLLTSYDSSDIDTRVRLEPSPENGLNKVSYVMTDKIVTVDRKL
LGYQVGVVDDAAMANIGRQLMRVLGLL

Download         Length: 324 bp

>T76573 NZ_CP021386:c984698-984375 [Bifidobacterium breve]
ATGAAACGAGGTGAGATTCGGACGTTGCAGGCCGATGGCTACGCCAGCAAGCCACGGCCGGTTGTCATCGTGCAGAGCGA
TGCCGTCGATCGGTTCGATTCCGTCATCACTTGTCTGCTGACCTCATACGATTCCTCCGACATCGACACGCGGGTCCGAT
TGGAACCAAGTCCGGAGAACGGCCTGAACAAAGTGAGTTACGTGATGACGGACAAGATCGTCACCGTCGACCGCAAACTG
CTGGGATACCAAGTCGGCGTGGTTGATGATGCAGCCATGGCGAATATCGGCCGTCAGCTCATGCGGGTTCTAGGTTTGCT
GTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9339.43 Da        Isoelectric Point: 4.5881

>AT76573 WP_003829112.1 NZ_CP021386:c984930-984685 [Bifidobacterium breve]
MSMQIATRVDDEQAALFKETTRQLGTTPADALRMFISAFNDYRGFPYEVRLPRNDVEPFASERDATEYASRLALRMSDET
R

Download         Length: 246 bp

>AT76573 NZ_CP021386:c984930-984685 [Bifidobacterium breve]
ATGAGCATGCAGATTGCCACGCGAGTTGATGACGAGCAGGCTGCGTTGTTTAAGGAGACGACCCGACAGCTGGGTACCAC
CCCGGCCGATGCCCTGCGTATGTTCATCAGCGCGTTCAATGATTACCGTGGGTTCCCCTATGAGGTGCGTTTGCCACGAA
ATGACGTGGAGCCGTTTGCGTCCGAGCGGGACGCTACTGAGTATGCCAGTCGTCTCGCCTTGAGGATGTCCGATGAAACG
AGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4R0SL31

Antitoxin

Source ID Structure
AlphaFold DB A0A087B6Q6

References