Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/RelB(antitoxin)
Location 1026454..1027090 Replicon chromosome
Accession NZ_CP021384
Organism Bifidobacterium breve strain NRBB01

Toxin (Protein)


Gene name mazF Uniprot ID D4BP71
Locus tag NRBB01_RS04550 Protein ID WP_003829234.1
Coordinates 1026454..1026810 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID D6PAX1
Locus tag NRBB01_RS04555 Protein ID WP_012577921.1
Coordinates 1026797..1027090 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NRBB01_RS04520 1021646..1022557 + 912 WP_003829224.1 ABC transporter ATP-binding protein -
NRBB01_RS04525 1022754..1023380 + 627 WP_003829226.1 30S ribosomal protein S4 -
NRBB01_RS04530 1023717..1024499 - 783 WP_003829227.1 gamma-glutamyl-gamma-aminobutyrate hydrolase family protein -
NRBB01_RS04535 1024640..1025125 - 486 WP_003829229.1 cytidine deaminase -
NRBB01_RS04540 1025214..1025570 + 357 WP_003829232.1 SdpI family protein -
NRBB01_RS04545 1025675..1026277 + 603 WP_003829233.1 transglutaminase family protein -
NRBB01_RS04550 1026454..1026810 - 357 WP_003829234.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
NRBB01_RS04555 1026797..1027090 - 294 WP_012577921.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
NRBB01_RS04560 1027241..1027405 - 165 Protein_842 IS30 family transposase -
NRBB01_RS04565 1027505..1028179 + 675 WP_032738178.1 histidine phosphatase family protein -
NRBB01_RS04570 1028262..1028666 + 405 WP_003829238.1 DUF948 domain-containing protein -
NRBB01_RS04575 1028666..1028902 + 237 WP_003829239.1 hypothetical protein -
NRBB01_RS04580 1028998..1031676 + 2679 WP_003829240.1 alanine--tRNA ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-110)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(11-53)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 13316.23 Da        Isoelectric Point: 7.9984

>T76569 WP_003829234.1 NZ_CP021384:c1026810-1026454 [Bifidobacterium breve]
MMKTDPHQFEIWWVPFTFPDKPGKAKNRPSVILQWDDGTRIALVTKVTGNTWRDEPGYVVLRDWQDAGLSKPSAVRCSQL
LRLPSSLFLDDGPTGRLSAYDAGRVTWAINELYPGLLA

Download         Length: 357 bp

>T76569 NZ_CP021384:c1026810-1026454 [Bifidobacterium breve]
ATGATGAAGACTGATCCGCACCAGTTCGAGATATGGTGGGTGCCGTTCACTTTTCCCGACAAGCCAGGCAAAGCGAAGAA
CCGTCCCAGCGTGATCCTCCAATGGGACGACGGCACCCGCATCGCCTTGGTGACCAAGGTCACCGGCAACACCTGGCGCG
ACGAACCGGGCTATGTGGTGCTGCGTGACTGGCAGGACGCGGGACTGAGCAAGCCCAGCGCCGTGCGATGCTCGCAACTG
CTGCGGCTGCCAAGCAGTCTTTTCCTCGACGACGGCCCGACAGGAAGACTTTCGGCATATGATGCCGGCCGCGTCACCTG
GGCGATCAACGAACTCTATCCGGGCCTACTAGCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10866.31 Da        Isoelectric Point: 4.2896

>AT76569 WP_012577921.1 NZ_CP021384:c1027090-1026797 [Bifidobacterium breve]
MAIKEKTTISLDAQTKRDGIAILDAMGLNLSTFAEMSLRQLVRDGRLPFTPSVRPSFEKDNEGYPLFKANMDDPRIVTPQ
IRDGAVILPEGWDDDED

Download         Length: 294 bp

>AT76569 NZ_CP021384:c1027090-1026797 [Bifidobacterium breve]
ATGGCTATCAAGGAGAAGACCACGATCAGTCTGGATGCGCAGACCAAGCGTGACGGCATCGCCATACTTGACGCCATGGG
GTTGAACCTGAGCACGTTTGCCGAAATGTCTCTCAGACAATTGGTACGCGACGGGCGTCTGCCATTCACGCCAAGCGTCC
GCCCTTCATTCGAAAAAGACAACGAGGGATATCCCCTGTTCAAAGCGAACATGGATGATCCCCGCATCGTGACGCCCCAG
ATACGTGATGGTGCCGTGATCCTGCCGGAGGGTTGGGACGATGATGAAGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0L7B6G7

Antitoxin

Source ID Structure
AlphaFold DB A0A1S2VTZ6

References