Detailed information of TA system TA076549

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 145919..146628
• Replicon: plasmid pACP4.2
• Accession: NZ_CP021368
• Organism: Acidovorax carolinensis strain P4

Toxin (Protein)

• Gene name: tad
• Uniprot ID: A0A210VR27
• Locus tag: CBP36_RS20880
• Protein ID: WP_011805136.1
• Coordinates: 146245..146628 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: CBP36_RS20875
• Protein ID: WP_011805135.1
• Coordinates: 145919..146248 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CBP36_RS20850	141423..142823	+	1401	WP_114655503.1	sodium:proton exchanger	-
CBP36_RS20860	143191..143511	-	321	WP_067513095.1	copper-binding protein	-
CBP36_RS20865	143902..144195	+	294	WP_049756471.1	MerR family DNA-binding protein	-
CBP36_RS20870	144206..145906	+	1701	WP_011805134.1	site-specific integrase	-
CBP36_RS20875	145919..146248	-	330	WP_011805135.1	helix-turn-helix domain-containing protein	Antitoxin
CBP36_RS20880	146245..146628	-	384	WP_011805136.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CBP36_RS20885	146847..147083	-	237	Protein_128	acyl-CoA dehydrogenase	-
CBP36_RS20890	147225..147923	+	699	WP_086929142.1	TetR/AcrR family transcriptional regulator	-
CBP36_RS20895	148017..149219	-	1203	WP_086929123.1	FAD-dependent oxidoreductase	-
CBP36_RS20900	149239..149559	-	321	WP_086929124.1	non-heme iron oxygenase ferredoxin subunit	-
CBP36_RS20905	149598..150740	-	1143	WP_086929125.1	aromatic ring-hydroxylating dioxygenase subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	sodB	1..163888	163888

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (18-110)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-90)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14062.15 Da
• Isoelectric Point: 10.0145

>T76549 WP_011805136.1 NZ_CP021368:c146628-146245 [Acidovorax carolinensis]
MSTQPRKPLKWVGSAKRDLDGMPEDVQDVFGHAIDLAQAGGKHPDAKALSGFGSAAVLEVVEDFRSDTFRAVYTVKFAGW
VYVLHCFQKKSKSGIKTPKEDLDLIKARLKAAVQDFEAWQAKQGVKR

Nucleotide

• Length: 384 bp

>T76549 NZ_CP021368:c146628-146245 [Acidovorax carolinensis]
ATGTCCACCCAGCCGCGCAAACCGCTCAAATGGGTCGGCTCGGCCAAGCGGGATCTGGACGGAATGCCCGAGGACGTCCA
AGACGTGTTCGGCCATGCGATCGACCTGGCGCAAGCCGGTGGCAAGCACCCGGACGCCAAGGCGCTGAGCGGGTTTGGTT
CCGCCGCGGTTTTGGAAGTGGTCGAGGATTTCCGGAGCGACACGTTCCGGGCGGTCTACACGGTCAAGTTCGCGGGCTGG
GTCTATGTGCTGCACTGTTTTCAGAAGAAGTCCAAGAGCGGCATCAAGACGCCGAAGGAGGACCTGGACCTGATCAAGGC
ACGGCTGAAGGCTGCGGTGCAGGATTTTGAGGCTTGGCAAGCAAAGCAAGGAGTAAAGCGATGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11929.73 Da
• Isoelectric Point: 10.5281

>AT76549 WP_011805135.1 NZ_CP021368:c146248-145919 [Acidovorax carolinensis]
MSQNSHITIEEGSTNVYADLGYADAAEMQRKSQLAAEIARAIKARRLTQQGAAALLEIDQSKISRITRGQFRGVSEAKLL
ELVAKLGHDVKIVVGPVRRRAGKIELQFA

Nucleotide

• Length: 330 bp

>AT76549 NZ_CP021368:c146248-145919 [Acidovorax carolinensis]
ATGAGCCAGAACAGCCACATCACCATCGAAGAAGGCAGCACCAACGTCTACGCGGACCTGGGCTATGCCGACGCTGCGGA
AATGCAGCGCAAGTCGCAGCTCGCGGCTGAGATTGCCCGCGCCATCAAGGCGCGACGCCTGACGCAACAGGGAGCCGCCG
CGCTGCTGGAAATCGATCAGTCCAAGATCTCCCGCATCACGCGCGGCCAGTTTCGCGGGGTCAGCGAGGCGAAGCTGCTG
GAACTGGTGGCCAAGCTGGGGCATGACGTGAAGATCGTGGTCGGTCCAGTACGGCGGCGTGCCGGTAAGATCGAGCTGCA
GTTCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A210VR27