Detailed information of TA system TA076545

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 8506..9215
• Replicon: plasmid pACP3.2
• Accession: NZ_CP021364
• Organism: Acidovorax carolinensis strain P3

Toxin (Protein)

• Gene name: tad
• Uniprot ID: A0A210VR27
• Locus tag: CBP35_RS20255
• Protein ID: WP_011805136.1
• Coordinates: 8832..9215 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: CBP35_RS20250
• Protein ID: WP_011805135.1
• Coordinates: 8506..8835 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CBP35_RS20225	4010..5410	+	1401	WP_114655503.1	sodium:proton exchanger	-
CBP35_RS20235	5778..6098	-	321	WP_067513095.1	copper-binding protein	-
CBP35_RS20240	6489..6782	+	294	WP_049756471.1	MerR family DNA-binding protein	-
CBP35_RS20245	6793..8493	+	1701	WP_011805134.1	site-specific integrase	-
CBP35_RS20250	8506..8835	-	330	WP_011805135.1	helix-turn-helix domain-containing protein	Antitoxin
CBP35_RS20255	8832..9215	-	384	WP_011805136.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CBP35_RS20260	9434..9670	-	237	Protein_8	acyl-CoA dehydrogenase	-
CBP35_RS20265	9812..10510	+	699	WP_086929142.1	TetR/AcrR family transcriptional regulator	-
CBP35_RS20270	10604..11806	-	1203	WP_086929123.1	FAD-dependent oxidoreductase	-
CBP35_RS20275	11826..12146	-	321	WP_086929124.1	non-heme iron oxygenase ferredoxin subunit	-
CBP35_RS20280	12185..13327	-	1143	WP_086929125.1	aromatic ring-hydroxylating dioxygenase subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	sodB	1..156922	156922

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (18-110)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-90)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14062.15 Da
• Isoelectric Point: 10.0145

>T76545 WP_011805136.1 NZ_CP021364:c9215-8832 [Acidovorax carolinensis]
MSTQPRKPLKWVGSAKRDLDGMPEDVQDVFGHAIDLAQAGGKHPDAKALSGFGSAAVLEVVEDFRSDTFRAVYTVKFAGW
VYVLHCFQKKSKSGIKTPKEDLDLIKARLKAAVQDFEAWQAKQGVKR

Nucleotide

• Length: 384 bp

>T76545 NZ_CP021364:c9215-8832 [Acidovorax carolinensis]
ATGTCCACCCAGCCGCGCAAACCGCTCAAATGGGTCGGCTCGGCCAAGCGGGATCTGGACGGAATGCCCGAGGACGTCCA
AGACGTGTTCGGCCATGCGATCGACCTGGCGCAAGCCGGTGGCAAGCACCCGGACGCCAAGGCGCTGAGCGGGTTTGGTT
CCGCCGCGGTTTTGGAAGTGGTCGAGGATTTCCGGAGCGACACGTTCCGGGCGGTCTACACGGTCAAGTTCGCGGGCTGG
GTCTATGTGCTGCACTGTTTTCAGAAGAAGTCCAAGAGCGGCATCAAGACGCCGAAGGAGGACCTGGACCTGATCAAGGC
ACGGCTGAAGGCTGCGGTGCAGGATTTTGAGGCTTGGCAAGCAAAGCAAGGAGTAAAGCGATGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11929.73 Da
• Isoelectric Point: 10.5281

>AT76545 WP_011805135.1 NZ_CP021364:c8835-8506 [Acidovorax carolinensis]
MSQNSHITIEEGSTNVYADLGYADAAEMQRKSQLAAEIARAIKARRLTQQGAAALLEIDQSKISRITRGQFRGVSEAKLL
ELVAKLGHDVKIVVGPVRRRAGKIELQFA

Nucleotide

• Length: 330 bp

>AT76545 NZ_CP021364:c8835-8506 [Acidovorax carolinensis]
ATGAGCCAGAACAGCCACATCACCATCGAAGAAGGCAGCACCAACGTCTACGCGGACCTGGGCTATGCCGACGCTGCGGA
AATGCAGCGCAAGTCGCAGCTCGCGGCTGAGATTGCCCGCGCCATCAAGGCGCGACGCCTGACGCAACAGGGAGCCGCCG
CGCTGCTGGAAATCGATCAGTCCAAGATCTCCCGCATCACGCGCGGCCAGTTTCGCGGGGTCAGCGAGGCGAAGCTGCTG
GAACTGGTGGCCAAGCTGGGGCATGACGTGAAGATCGTGGTCGGTCCAGTACGGCGGCGTGCCGGTAAGATCGAGCTGCA
GTTCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A210VR27