Detailed information of TA system TA076521

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 23430..24031
• Replicon: plasmid p95NR1A
• Accession: NZ_CP021340
• Organism: Escherichia coli strain 95NR1

Toxin (Protein)

• Gene name: doc
• Uniprot ID: U9YA20
• Locus tag: EC95NR1_RS29575
• Protein ID: WP_001216045.1
• Coordinates: 23651..24031 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: EC95NR1_RS29570
• Protein ID: WP_001190712.1
• Coordinates: 23430..23651 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EC95NR1_RS29505	18551..18739	+	189	WP_000797279.1	hypothetical protein	-
EC95NR1_RS29510	18741..18950	+	210	WP_000951710.1	hypothetical protein	-
EC95NR1_RS29515	18947..19489	+	543	WP_021351680.1	DUF551 domain-containing protein	-
EC95NR1_RS29520	19572..19805	+	234	WP_000516537.1	hypothetical protein	-
EC95NR1_RS29525	19984..20277	+	294	WP_000269003.1	hypothetical protein	-
EC95NR1_RS29530	20284..20658	+	375	WP_000988658.1	hypothetical protein	-
EC95NR1_RS29535	20640..21572	+	933	WP_000057449.1	hypothetical protein	-
EC95NR1_RS29540	21569..21931	+	363	WP_001261544.1	hypothetical protein	-
EC95NR1_RS31310	22006..22143	-	138	WP_000123562.1	hypothetical protein	-
EC95NR1_RS29555	22593..22844	+	252	WP_001377386.1	DNA polymerase III subunit theta	-
EC95NR1_RS29565	22968..23357	+	390	WP_000506726.1	DNA repair protein	-
EC95NR1_RS29570	23430..23651	+	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
EC95NR1_RS29575	23651..24031	+	381	WP_001216045.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
EC95NR1_RS29580	24036..24215	+	180	WP_000113019.1	hypothetical protein	-
EC95NR1_RS29585	24243..25286	+	1044	WP_000648825.1	DUF968 domain-containing protein	-
EC95NR1_RS29590	25375..25827	+	453	WP_001369802.1	hypothetical protein	-
EC95NR1_RS29595	25914..27107	+	1194	WP_000219604.1	terminase	-
EC95NR1_RS29600	27107..28591	+	1485	WP_000124155.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..86917	86917

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (3-120)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13588.29 Da
• Isoelectric Point: 5.1514

>T76521 WP_001216045.1 NZ_CP021340:23651-24031 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T76521 NZ_CP021340:23651-24031 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT76521 WP_001190712.1 NZ_CP021340:23430-23651 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT76521 NZ_CP021340:23430-23651 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• AlphaFold DB: A0A829CLP7

Antitoxin

• AlphaFold DB: A0A829CJB6