Detailed information of TA system TA076519

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 5301658..5302253
• Replicon: chromosome
• Accession: NZ_CP021339
• Organism: Escherichia coli strain 95NR1

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: -
• Locus tag: EC95NR1_RS28545
• Protein ID: WP_000239584.1
• Coordinates: 5301903..5302253 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: U9Y2K1
• Locus tag: EC95NR1_RS28540
• Protein ID: WP_001223208.1
• Coordinates: 5301658..5301909 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EC95NR1_RS28530	5297323..5301102	+	3780	WP_000060925.1	autotransporter assembly complex protein TamB	-
EC95NR1_RS28535	5301105..5301446	+	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
EC95NR1_RS28540	5301658..5301909	+	252	WP_001223208.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
EC95NR1_RS28545	5301903..5302253	+	351	WP_000239584.1	endoribonuclease toxin ChpB	Toxin
EC95NR1_RS28550	5302333..5302863	-	531	WP_000055075.1	inorganic diphosphatase	-
EC95NR1_RS28555	5303173..5304129	+	957	WP_000265933.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
EC95NR1_RS28560	5304269..5305771	+	1503	WP_000205815.1	sugar ABC transporter ATP-binding protein	-
EC95NR1_RS28565	5305785..5306807	+	1023	WP_001296689.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12527.45 Da
• Isoelectric Point: 5.6219

>T76519 WP_000239584.1 NZ_CP021339:5301903-5302253 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQSFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T76519 NZ_CP021339:5301903-5302253 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAATCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9271.58 Da
• Isoelectric Point: 4.4201

>AT76519 WP_001223208.1 NZ_CP021339:5301658-5301909 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT76519 NZ_CP021339:5301658-5301909 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LQ26