Detailed information of TA system TA076512

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3067878..3068503
• Replicon: chromosome
• Accession: NZ_CP021339
• Organism: Escherichia coli strain 95NR1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: EC95NR1_RS16625
• Protein ID: WP_000911330.1
• Coordinates: 3067878..3068276 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: EC95NR1_RS16630
• Protein ID: WP_000450524.1
• Coordinates: 3068276..3068503 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EC95NR1_RS16605	3063756..3063956	+	201	WP_000383836.1	YpfN family protein	-
EC95NR1_RS16610	3064066..3064764	-	699	WP_000679812.1	esterase	-
EC95NR1_RS16615	3064838..3066853	-	2016	WP_000829332.1	tRNA cytosine(34) acetyltransferase TmcA	-
EC95NR1_RS16620	3066868..3067731	-	864	WP_001267508.1	neutral zinc metallopeptidase	-
EC95NR1_RS16625	3067878..3068276	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
EC95NR1_RS16630	3068276..3068503	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
EC95NR1_RS16635	3068658..3069371	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
EC95NR1_RS16640	3069584..3070618	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
EC95NR1_RS16645	3070635..3071513	-	879	WP_001311023.1	4-hydroxy-tetrahydrodipicolinate synthase	-
EC95NR1_RS16650	3071659..3072237	+	579	WP_000176193.1	glycine cleavage system transcriptional repressor	-
EC95NR1_RS16655	3072237..3072707	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T76512 WP_000911330.1 NZ_CP021339:c3068276-3067878 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T76512 NZ_CP021339:c3068276-3067878 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT76512 WP_000450524.1 NZ_CP021339:c3068503-3068276 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT76512 NZ_CP021339:c3068503-3068276 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33