Detailed information of TA system TA076492

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 23431..24032
• Replicon: plasmid p95JB1A
• Accession: NZ_CP021336
• Organism: Escherichia coli strain 95JB1

Toxin (Protein)

• Gene name: doc
• Uniprot ID: U9YA20
• Locus tag: EC95JB1_RS28730
• Protein ID: WP_001216045.1
• Coordinates: 23652..24032 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: EC95JB1_RS28725
• Protein ID: WP_001190712.1
• Coordinates: 23431..23652 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EC95JB1_RS28660	18552..18740	+	189	WP_000797279.1	hypothetical protein	-
EC95JB1_RS28665	18742..18951	+	210	WP_000951710.1	hypothetical protein	-
EC95JB1_RS28670	18948..19490	+	543	WP_021351680.1	DUF551 domain-containing protein	-
EC95JB1_RS28675	19573..19806	+	234	WP_000516537.1	hypothetical protein	-
EC95JB1_RS28680	19985..20278	+	294	WP_000269003.1	hypothetical protein	-
EC95JB1_RS28685	20285..20659	+	375	WP_000988658.1	hypothetical protein	-
EC95JB1_RS28690	20641..21573	+	933	WP_000057449.1	hypothetical protein	-
EC95JB1_RS28695	21570..21932	+	363	WP_001261544.1	hypothetical protein	-
EC95JB1_RS30395	22007..22144	-	138	WP_000123562.1	hypothetical protein	-
EC95JB1_RS28710	22594..22845	+	252	WP_001377386.1	DNA polymerase III subunit theta	-
EC95JB1_RS28720	22969..23358	+	390	WP_000506726.1	DNA repair protein	-
EC95JB1_RS28725	23431..23652	+	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
EC95JB1_RS28730	23652..24032	+	381	WP_001216045.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
EC95JB1_RS28735	24037..24216	+	180	WP_000113019.1	hypothetical protein	-
EC95JB1_RS28740	24244..25287	+	1044	WP_000648825.1	DUF968 domain-containing protein	-
EC95JB1_RS28745	25376..25828	+	453	WP_001369802.1	hypothetical protein	-
EC95JB1_RS28750	25915..27108	+	1194	WP_000219604.1	terminase	-
EC95JB1_RS28755	27108..28592	+	1485	WP_000124155.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..86922	86922

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (3-120)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13588.29 Da
• Isoelectric Point: 5.1514

>T76492 WP_001216045.1 NZ_CP021336:23652-24032 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T76492 NZ_CP021336:23652-24032 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT76492 WP_001190712.1 NZ_CP021336:23431-23652 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT76492 NZ_CP021336:23431-23652 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• AlphaFold DB: A0A829CLP7

Antitoxin

• AlphaFold DB: A0A829CJB6