Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 5062983..5063480 Replicon chromosome
Accession NZ_CP021335
Organism Escherichia coli strain 95JB1

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag EC95JB1_RS27080 Protein ID WP_001173343.1
Coordinates 5063307..5063480 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag EC95JB1_RS27075 Protein ID WP_000398619.1
Coordinates 5062983..5063279 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EC95JB1_RS27050 5059714..5060433 - 720 WP_000611288.1 two-component system response regulator DcuR -
EC95JB1_RS27055 5060430..5062061 - 1632 WP_001216487.1 sensor histidine kinase -
EC95JB1_RS27060 5062242..5062472 + 231 WP_000371700.1 (4Fe-4S)-binding protein -
EC95JB1_RS27065 5062484..5062756 + 273 WP_000405647.1 N-acetyltransferase -
EC95JB1_RS27075 5062983..5063279 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
EC95JB1_RS27080 5063307..5063480 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
EC95JB1_RS27085 5063599..5063970 - 372 Protein_4947 lysine--tRNA ligase -
EC95JB1_RS27090 5064027..5065240 + 1214 WP_162829202.1 IS3 family transposase -
EC95JB1_RS30095 5065276..5065437 - 162 Protein_4949 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T76489 WP_001173343.1 NZ_CP021335:5063307-5063480 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T76489 NZ_CP021335:5063307-5063480 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT76489 WP_000398619.1 NZ_CP021335:5062983-5063279 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT76489 NZ_CP021335:5062983-5063279 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References