Detailed information of TA system TA076461

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 4193491..4195094
• Replicon: chromosome
• Accession: NZ_CP021329
• Organism: Raoultella ornithinolytica strain Ro24724

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: CA210_RS22810
• Protein ID: WP_088883968.1
• Coordinates: 4193491..4194822 (-)
• Length: 444 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A808YAX5
• Locus tag: CA210_RS22815
• Protein ID: WP_032717448.1
• Coordinates: 4194822..4195094 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA210_RS22795	4189365..4190279	-	915	WP_088883967.1	branched-chain amino acid ABC transporter permease LivH	-
CA210_RS22800	4190379..4191437	-	1059	WP_162180097.1	branched-chain amino acid ABC transporter substrate-binding protein	-
CA210_RS22805	4192152..4193558	+	1407	WP_088884164.1	glycoside hydrolase family 10 protein	-
CA210_RS22810	4193491..4194822	-	1332	WP_088883968.1	type II toxin-antitoxin system HipA family toxin	Toxin
CA210_RS22815	4194822..4195094	-	273	WP_032717448.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
CA210_RS22820	4195298..4195852	-	555	WP_004861926.1	RNA polymerase sigma factor	-
CA210_RS22825	4195858..4196283	-	426	WP_032724724.1	hypothetical protein	-
CA210_RS22830	4196416..4197369	-	954	WP_004861921.1	hypothetical protein	-
CA210_RS22835	4197583..4198215	-	633	WP_048023555.1	class I SAM-dependent methyltransferase	-
CA210_RS22840	4198235..4199476	-	1242	WP_088883969.1	sensor domain-containing diguanylate cyclase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4172114..4338946	166832

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-402)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 444 a.a.
• Molecular weight: 49360.69 Da
• Isoelectric Point: 6.5073

>T76461 WP_088883968.1 NZ_CP021329:c4194822-4193491 [Raoultella ornithinolytica]
MATLVTWMNNERVGELIKQASGAHLFRYEEAWMRNPRARPLSLSLPLQYGNITDEAVFNYFDNLLPDSPRVRDRIVKRYQ
ARSKQPFDLLAEIGRDSVGAVTLLPPGENAPRGALSWELLDNHDLDALLTAYQSDIPLGMVNEHDDFRISVAGAQEKTAL
LKIGEQWCIPTGTTPTTHIIKLPIGEIKQPDAVLDLSESVDNEYLCLALARALGFAVPPASIIQTASIRALAVERFDRRW
AQEKTVLLRLPQEDLCQAFGIPSSIKYESDGGPGIRAIMAFLVGSSEALEDRYNFMKFMVFQWLIGATDGHAKNFSIFLQ
PGGSYRLTPFYDIISAFPLLGGKGLHLSDLKLSMSLKASKGRKTEIQTLYPRHFLATAKEVGFAQQQMLEILRYFVDNVP
RAVEAVKGTLPPDFSQPVYEAITDRLLWVHSRLQNAMAASPSG

Nucleotide

• Length: 1332 bp

>T76461 NZ_CP021329:c4194822-4193491 [Raoultella ornithinolytica]
ATGGCGACTCTCGTGACATGGATGAACAATGAGCGGGTCGGCGAGCTAATCAAGCAGGCGAGCGGCGCACATCTCTTTCG
CTATGAAGAGGCGTGGATGCGCAATCCTCGCGCCAGACCGCTCTCACTGTCGCTTCCGCTGCAGTACGGTAACATCACCG
ACGAGGCGGTATTTAACTACTTTGACAATCTGCTGCCGGACAGTCCGCGGGTACGGGACCGCATTGTTAAGCGCTATCAG
GCGCGTTCAAAGCAGCCGTTTGATCTGCTGGCCGAGATCGGACGGGACAGCGTCGGGGCGGTAACGTTACTCCCGCCAGG
AGAAAATGCGCCCCGCGGGGCGTTGAGCTGGGAGCTGCTGGATAACCACGATCTGGATGCGTTACTGACGGCCTACCAGT
CGGATATACCGCTCGGAATGGTCAACGAACACGATGATTTCAGGATCTCGGTGGCCGGGGCCCAGGAGAAAACCGCGCTG
CTGAAAATCGGCGAGCAGTGGTGTATCCCGACGGGTACCACGCCGACCACCCACATCATTAAACTGCCTATCGGCGAGAT
CAAACAGCCGGATGCGGTGCTGGATCTCAGCGAAAGCGTCGATAACGAATATTTGTGTCTGGCGCTGGCCCGGGCGCTGG
GTTTTGCCGTTCCGCCCGCCAGCATTATCCAGACAGCCAGTATCAGGGCGCTGGCGGTGGAGCGTTTCGACCGTCGCTGG
GCGCAGGAGAAAACGGTGCTGCTGCGTTTGCCGCAGGAAGATCTGTGTCAGGCCTTCGGCATTCCGTCATCGATTAAGTA
TGAGTCCGACGGCGGGCCGGGCATTCGGGCAATTATGGCTTTTCTGGTTGGCTCCAGCGAGGCACTCGAGGATCGCTATA
ACTTTATGAAGTTTATGGTGTTCCAGTGGCTGATTGGCGCAACCGATGGCCACGCCAAAAACTTCTCGATATTCCTCCAG
CCGGGCGGCAGCTACCGCTTAACCCCGTTCTACGACATCATCTCCGCGTTTCCGCTGCTTGGCGGAAAGGGACTGCACCT
GAGCGATCTCAAGCTGTCGATGAGTCTGAAGGCCAGTAAAGGACGTAAAACGGAGATCCAGACGCTCTATCCGCGTCACT
TTCTCGCTACGGCTAAAGAGGTAGGGTTTGCCCAACAGCAGATGCTGGAGATTCTGCGCTATTTTGTCGATAACGTTCCC
CGCGCGGTTGAGGCGGTAAAGGGGACTTTACCGCCTGACTTTTCACAACCGGTATATGAGGCGATCACCGATCGCTTACT
GTGGGTCCATTCACGCTTGCAGAACGCAATGGCGGCGTCTCCGTCGGGCTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10365.71 Da
• Isoelectric Point: 5.7971

>AT76461 WP_032717448.1 NZ_CP021329:c4195094-4194822 [Raoultella ornithinolytica]
MSPSPTIYSPVQLANYLKLVRQKNQWTQSELAQRMGIKQATVSNFENHPEKTTLTTVFKILQSLELSMAICEKNAPDTDD
NRDNPRDLEW

Nucleotide

• Length: 273 bp

>AT76461 NZ_CP021329:c4195094-4194822 [Raoultella ornithinolytica]
ATGAGCCCTTCACCAACGATATACAGCCCGGTTCAGCTGGCAAACTATCTCAAGCTGGTGCGCCAGAAAAACCAGTGGAC
GCAGAGCGAGCTGGCTCAGCGTATGGGGATTAAACAGGCCACCGTCTCTAACTTTGAAAATCATCCCGAGAAAACGACGT
TAACCACGGTGTTTAAAATTTTACAGTCACTGGAACTGTCGATGGCGATTTGCGAAAAAAATGCGCCGGATACCGACGAT
AACCGTGATAACCCGCGGGATCTGGAGTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A808YAX5