Detailed information of TA system TA076448

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 644565..645141
• Replicon: chromosome
• Accession: NZ_CP021329
• Organism: Raoultella ornithinolytica strain Ro24724

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: CA210_RS05780
• Protein ID: WP_015585223.1
• Coordinates: 644854..645141 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: CA210_RS05775
• Protein ID: WP_069476272.1
• Coordinates: 644565..644867 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA210_RS05760	640504..640839	+	336	WP_088883471.1	endoribonuclease SymE	-
CA210_RS05765	641070..643142	+	2073	WP_069476270.1	AAA family ATPase	-
CA210_RS05770	643142..644464	+	1323	WP_069476271.1	restriction endonuclease	-
CA210_RS05775	644565..644867	-	303	WP_069476272.1	BrnA antitoxin family protein	Antitoxin
CA210_RS05780	644854..645141	-	288	WP_015585223.1	BrnT family toxin	Toxin
CA210_RS05785	645258..645677	-	420	WP_015585224.1	FosA family fosfomycin resistance glutathione transferase	-
CA210_RS05790	645671..646579	-	909	WP_004857513.1	LysR family transcriptional regulator	-
CA210_RS05795	646667..647449	+	783	WP_088883472.1	NAD(P)H-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (40-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11067.49 Da
• Isoelectric Point: 7.0747

>T76448 WP_015585223.1 NZ_CP021329:c645141-644854 [Raoultella ornithinolytica]
MPMEFEWDANKAASNLRKHGIRFEEAVLVFDDPQHLSRQDRYENGEHRWQTIGLVHGIIVILVVHSVRFESGAEVIRIIS
ARKADGKERSRYEHS

Nucleotide

• Length: 288 bp

>T76448 NZ_CP021329:c645141-644854 [Raoultella ornithinolytica]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCAGCAAGCAATCTAAGAAAACATGGGATTCGCTTCGAGGAGGCGGT
ACTGGTTTTTGACGATCCGCAGCATCTTTCCCGCCAGGATCGTTATGAAAACGGTGAGCATCGATGGCAAACCATCGGCC
TCGTTCATGGAATTATCGTTATTCTTGTGGTGCATTCGGTCCGCTTTGAAAGCGGGGCTGAGGTGATTCGTATCATCAGC
GCCCGGAAGGCGGACGGCAAAGAGAGGAGTCGTTATGAGCACAGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11282.70 Da
• Isoelectric Point: 9.4162

>AT76448 WP_069476272.1 NZ_CP021329:c644867-644565 [Raoultella ornithinolytica]
MSTVKYKRDNPAGLSAQREDELRALANKADGEIDYSDIPASGDEQWPDAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMNRDLGGK

Nucleotide

• Length: 303 bp

>AT76448 NZ_CP021329:c644867-644565 [Raoultella ornithinolytica]
ATGAGCACAGTTAAATACAAGCGCGACAATCCGGCCGGGCTTAGCGCTCAGCGTGAAGACGAGCTCAGAGCGCTGGCGAA
CAAAGCGGATGGCGAAATTGATTACAGCGATATCCCCGCTTCAGGTGATGAACAGTGGCCGGACGCGGTACGCGGTAAAT
TCTACCGACCGTTGAAAACCCAGGCCTCTGTCCGCATCGACGCTGATGTGATGGAGTGGCTTAAACGCCCGGGAAAAGGC
TATCAAACCCGGCTGAACGCGATTTTAAGAGAAGCGATGAACCGCGATCTGGGTGGGAAATAA