Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 112089..112624 Replicon plasmid pPA45B
Accession NZ_CP021289
Organism Escherichia coli strain PA45B

Toxin (Protein)


Gene name relE Uniprot ID A0A0M1UJC2
Locus tag PA45B_RS27025 Protein ID WP_001270422.1
Coordinates 112337..112624 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID L4J0V6
Locus tag PA45B_RS27020 Protein ID WP_001132895.1
Coordinates 112089..112340 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PA45B_RS26980 107106..107417 - 312 WP_001309256.1 hypothetical protein -
PA45B_RS27850 107481..107840 - 360 WP_001336695.1 DUF1281 domain-containing protein -
PA45B_RS27855 107847..107990 - 144 WP_000270807.1 DUF1281 domain-containing protein -
PA45B_RS27000 108157..108933 - 777 Protein_129 hypothetical protein -
PA45B_RS27005 109167..110129 - 963 WP_000756328.1 ParB/RepB/Spo0J family partition protein -
PA45B_RS27010 110126..111331 - 1206 WP_000817635.1 AAA family ATPase -
PA45B_RS27020 112089..112340 + 252 WP_001132895.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
PA45B_RS27025 112337..112624 + 288 WP_001270422.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
PA45B_RS27030 112626..113037 + 412 Protein_134 DDE-type integrase/transposase/recombinase -
PA45B_RS27040 113681..114658 + 978 WP_001309253.1 RepB family plasmid replication initiator protein -
PA45B_RS27045 114901..115641 - 741 WP_001066947.1 tyrosine-type recombinase/integrase -
PA45B_RS27050 115762..115950 - 189 WP_001332784.1 hypothetical protein -
PA45B_RS27055 116317..117486 + 1170 WP_001072358.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid ant(3'')-Ia / qacE / sul1 / blaTEM-1B senB 1..147172 147172
- flank IS/Tn - - 108370..108510 140


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11262.30 Da        Isoelectric Point: 10.4703

>T76439 WP_001270422.1 NZ_CP021289:112337-112624 [Escherichia coli]
MRYQVKFREDALKEWQKMDKTIQQQFAKKLKKCCDNPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGK
RERSDVYNLASERMR

Download         Length: 288 bp

>T76439 NZ_CP021289:112337-112624 [Escherichia coli]
ATGAGGTATCAGGTAAAATTCAGGGAAGATGCGCTGAAAGAGTGGCAAAAAATGGACAAGACTATTCAGCAACAGTTTGC
GAAAAAGCTAAAAAAGTGCTGCGACAATCCGCATATTCCTTCCGCAAAACTGCGCGGGATAAAGGACTGCTACAAAATAA
AATTACGTGCGTCAGGTTTTCGCCTGGTCTATCAGGTGATTGACGAACAATTAATTATCGCTGTTGTAGCTGTGGGTAAA
CGTGAGCGCAGTGACGTTTATAATCTTGCCAGTGAAAGAATGAGATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9178.47 Da        Isoelectric Point: 4.2108

>AT76439 WP_001132895.1 NZ_CP021289:112089-112340 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLD
KYL

Download         Length: 252 bp

>AT76439 NZ_CP021289:112089-112340 [Escherichia coli]
ATGCCTAACATTATTCTCAGTGATACAAGCGCCAGTGTCAGCGAGCTGAAGAAAAATCCGATGGCGACAGTCAGCGCCGG
TGATGGTTTCCCGGTCGCTATCCTGAACCGTAATCAGCCTGCTTTCTACTGTGTACCCGCAGAGCTGTACGAAAAGATGC
TCGATGCCCTGGACGATCAGGAGCTGGTTAAACTGGTAGCCGAACGCAGCAACCAACCGCTGCATGATGTAGACCTGGAT
AAATACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M1UJC2

Antitoxin

Source ID Structure
AlphaFold DB L4J0V6

References