Detailed information of TA system TA076429

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4984313..4985133
• Replicon: chromosome
• Accession: NZ_CP021288
• Organism: Escherichia coli strain PA45B

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: B6I030
• Locus tag: PA45B_RS25775
• Protein ID: WP_001054379.1
• Coordinates: 4984876..4985133 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: -
• Locus tag: PA45B_RS25770
• Protein ID: WP_000123957.1
• Coordinates: 4984313..4984864 (-)
• Length: 184 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PA45B_RS25740	4980007..4981320	-	1314	WP_000460845.1	galactitol-specific PTS transporter subunit IIC	-
PA45B_RS25745	4981332..4981610	-	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
PA45B_RS25750	4981607..4982728	-	1122	WP_000010833.1	M42 family metallopeptidase	-
PA45B_RS27835	4982973..4983089	-	117	Protein_4708	VOC family protein	-
PA45B_RS25760	4983127..4983387	-	261	WP_000077645.1	hypothetical protein	-
PA45B_RS25765	4983515..4984261	-	747	WP_000354249.1	class I SAM-dependent methyltransferase	-
PA45B_RS25770	4984313..4984864	-	552	WP_000123957.1	N-acetyltransferase	Antitoxin
PA45B_RS25775	4984876..4985133	-	258	WP_001054379.1	YjhX family toxin	Toxin
PA45B_RS25785	4985510..4985809	-	300	WP_001332034.1	GNAT family N-acetyltransferase	-
PA45B_RS25790	4985887..4987113	+	1227	Protein_4714	helicase YjhR	-
PA45B_RS25800	4987696..4988676	-	981	WP_000991462.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
PA45B_RS25805	4988740..4989846	-	1107	WP_001332032.1	N-acetylneuraminate epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (19-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9568.07 Da
• Isoelectric Point: 11.1381

>T76429 WP_001054379.1 NZ_CP021288:c4985133-4984876 [Escherichia coli]
MNLSRQEQRTLHVLAKGGRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTGLNNVRA
QLDNR

Nucleotide

• Length: 258 bp

>T76429 NZ_CP021288:c4985133-4984876 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACGTACGTTACACGTTCTCGCTAAAGGTGGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGTCAGCCCTATCGAATCAACACTACCGGGCTGAATAACGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 184 a.a.
• Molecular weight: 20358.34 Da
• Isoelectric Point: 6.4182

>AT76429 WP_000123957.1 NZ_CP021288:c4984864-4984313 [Escherichia coli]
MTAHHFTFQITDESDASDIREVETRAFGFSKEADLTASLLEDESARPALSLLARHEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQALTA
QPMNVTGHIQCAQALMKPEHWRE

Nucleotide

• Length: 552 bp

>AT76429 NZ_CP021288:c4984864-4984313 [Escherichia coli]
ATGACAGCACACCACTTCACTTTTCAAATCACAGATGAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAACGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCTGCGTTATCGCTGCTGGCCC
GACATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGGGAAATGGATTCACCGCTGATGCAT
ATCCTCGCTCCCCTGGCAGTCATACCGGAATACCAGGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAAGCACTCACCGCT
CAGCCCATGAACGTGACCGGCCACATCCAGTGCGCGCAGGCCCTGATGAAACCGGAGCACTGGCGTGAATAA

Structures

Toxin

• AlphaFold DB: B6I030