Detailed information of TA system TA076428

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4951296..4952130
• Replicon: chromosome
• Accession: NZ_CP021288
• Organism: Escherichia coli strain PA45B

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1J6XFW9
• Locus tag: PA45B_RS25600
• Protein ID: WP_000854770.1
• Coordinates: 4951753..4952130 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: PA45B_RS25595
• Protein ID: WP_001332042.1
• Coordinates: 4951296..4951664 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PA45B_RS25560	4948420..4948578	+	159	WP_001323397.1	DUF905 family protein	-
PA45B_RS25570	4948733..4949551	+	819	WP_001234738.1	DUF945 domain-containing protein	-
PA45B_RS25580	4949893..4950357	+	465	WP_000855061.1	antirestriction protein	-
PA45B_RS25585	4950373..4950849	+	477	WP_001557203.1	RadC family protein	-
PA45B_RS25590	4950912..4951133	+	222	WP_000692323.1	DUF987 domain-containing protein	-
PA45B_RS25595	4951296..4951664	+	369	WP_001332042.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
PA45B_RS25600	4951753..4952130	+	378	WP_000854770.1	TA system toxin CbtA family protein	Toxin
PA45B_RS25605	4952127..4952615	+	489	WP_000761690.1	hypothetical protein	-
PA45B_RS25610	4952632..4952808	+	177	WP_000839286.1	DUF957 domain-containing protein	-
PA45B_RS27630	4952908..4953066	+	159	WP_001467148.1	hypothetical protein	-
PA45B_RS27635	4953430..4953606	+	177	Protein_4684	helix-turn-helix domain-containing protein	-
PA45B_RS25625	4954088..4955625	+	1538	WP_085953974.1	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14196.37 Da
• Isoelectric Point: 8.7438

>T76428 WP_000854770.1 NZ_CP021288:4951753-4952130 [Escherichia coli]
MKTLPDTHVRKVSRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T76428 NZ_CP021288:4951753-4952130 [Escherichia coli]
ATGAAAACATTACCTGATACACACGTACGGAAGGTATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGTACCGACCTGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGATCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13677.38 Da
• Isoelectric Point: 6.5931

>AT76428 WP_001332042.1 NZ_CP021288:4951296-4951664 [Escherichia coli]
VSDTLSGTTHPDDNHDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYHPDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAFYPTSETKK

Nucleotide

• Length: 369 bp

>AT76428 NZ_CP021288:4951296-4951664 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAACGCATCCCGATGACAATCACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCACCCGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAATTAATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGTTACGTTTATCTGGCTTTTTATCCAACATCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A1J6XFW9