Detailed information of TA system TA076427

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4693276..4694110
• Replicon: chromosome
• Accession: NZ_CP021288
• Organism: Escherichia coli strain PA45B

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B7MK10
• Locus tag: PA45B_RS24160
• Protein ID: WP_000854820.1
• Coordinates: 4693276..4693653 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7MK11
• Locus tag: PA45B_RS24165
• Protein ID: WP_001285610.1
• Coordinates: 4693742..4694110 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PA45B_RS24140	4689839..4691377	-	1539	WP_001187191.1	lysine decarboxylation/transport transcriptional activator CadC	-
PA45B_RS24150	4692212..4692772	-	561	Protein_4422	DUF4942 domain-containing protein	-
PA45B_RS24155	4692857..4693054	-	198	WP_095591310.1	DUF957 domain-containing protein	-
PA45B_RS27595	4693082..4693279	-	198	Protein_4424	hypothetical protein	-
PA45B_RS24160	4693276..4693653	-	378	WP_000854820.1	type IV toxin-antitoxin system toxin CbtA	Toxin
PA45B_RS24165	4693742..4694110	-	369	WP_001285610.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
PA45B_RS24170	4694190..4694411	-	222	WP_000692347.1	DUF987 domain-containing protein	-
PA45B_RS24175	4694498..4694974	-	477	WP_001186193.1	RadC family protein	-
PA45B_RS24180	4694989..4695468	-	480	WP_000706978.1	antirestriction protein	-
PA45B_RS24185	4695734..4696552	-	819	WP_001175155.1	DUF945 domain-containing protein	-
PA45B_RS24190	4696642..4696875	-	234	WP_000883181.1	DUF905 family protein	-
PA45B_RS24195	4696881..4697558	-	678	WP_001097565.1	hypothetical protein	-
PA45B_RS24200	4697709..4698389	-	681	WP_001278649.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14043.12 Da
• Isoelectric Point: 7.8839

>T76427 WP_000854820.1 NZ_CP021288:c4693653-4693276 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNIILGKHPEVKQ

Nucleotide

• Length: 378 bp

>T76427 NZ_CP021288:c4693653-4693276 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGATCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAATATTATCCTGGGTAAGCACCCGGAGGTGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13606.39 Da
• Isoelectric Point: 6.0618

>AT76427 WP_001285610.1 NZ_CP021288:c4694110-4693742 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT76427 NZ_CP021288:c4694110-4693742 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGTACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGTGCCCGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0E2KXX6