Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3205989..3206643 Replicon chromosome
Accession NZ_CP021288
Organism Escherichia coli strain PA45B

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag PA45B_RS16530 Protein ID WP_000244781.1
Coordinates 3205989..3206396 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag PA45B_RS16535 Protein ID WP_000354046.1
Coordinates 3206377..3206643 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PA45B_RS16510 3201946..3203679 - 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -
PA45B_RS16515 3203685..3204395 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
PA45B_RS16520 3204420..3205316 - 897 WP_000806987.1 site-specific tyrosine recombinase XerD -
PA45B_RS16525 3205428..3205949 + 522 WP_001055874.1 flavodoxin FldB -
PA45B_RS16530 3205989..3206396 - 408 WP_000244781.1 protein YgfX Toxin
PA45B_RS16535 3206377..3206643 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
PA45B_RS16540 3206886..3207866 + 981 WP_000886078.1 tRNA-modifying protein YgfZ -
PA45B_RS16545 3207943..3208602 - 660 WP_000250274.1 hemolysin III family protein -
PA45B_RS16550 3208766..3209077 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
PA45B_RS16555 3209122..3210555 + 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T76423 WP_000244781.1 NZ_CP021288:c3206396-3205989 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T76423 NZ_CP021288:c3206396-3205989 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT76423 WP_000354046.1 NZ_CP021288:c3206643-3206377 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT76423 NZ_CP021288:c3206643-3206377 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References