Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2169381..2170212 Replicon chromosome
Accession NZ_CP021288
Organism Escherichia coli strain PA45B

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag PA45B_RS11365 Protein ID WP_000854814.1
Coordinates 2169838..2170212 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID S1PWQ3
Locus tag PA45B_RS11360 Protein ID WP_001285586.1
Coordinates 2169381..2169749 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PA45B_RS11320 2164598..2165668 + 1071 WP_000102631.1 patatin-like phospholipase family protein -
PA45B_RS11325 2165804..2166481 + 678 WP_001362823.1 hypothetical protein -
PA45B_RS11330 2166497..2166907 + 411 WP_000846704.1 hypothetical protein -
PA45B_RS11340 2167128..2167949 + 822 WP_001234569.1 DUF945 domain-containing protein -
PA45B_RS11345 2168040..2168525 + 486 WP_000213703.1 antirestriction protein -
PA45B_RS11350 2168541..2169017 + 477 WP_001351157.1 RadC family protein -
PA45B_RS11355 2169086..2169307 + 222 WP_000692323.1 DUF987 domain-containing protein -
PA45B_RS11360 2169381..2169749 + 369 WP_001285586.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
PA45B_RS11365 2169838..2170212 + 375 WP_000854814.1 type IV toxin-antitoxin system toxin CbtA Toxin
PA45B_RS11370 2170209..2170403 + 195 WP_000988601.1 hypothetical protein -
PA45B_RS27465 2170416..2170529 + 114 WP_001161660.1 DUF957 domain-containing protein -
PA45B_RS11375 2170818..2170964 - 147 Protein_2076 transposase domain-containing protein -
PA45B_RS11380 2171018..2171200 + 183 WP_001016348.1 hypothetical protein -
PA45B_RS11385 2171301..2171630 - 330 WP_000450409.1 DUF496 family protein -
PA45B_RS11390 2171802..2172860 - 1059 WP_001200890.1 FUSC family protein -
PA45B_RS11395 2173058..2173531 - 474 WP_001105376.1 DNA gyrase inhibitor SbmC -
PA45B_RS11400 2173650..2174816 - 1167 WP_001297905.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T76420 WP_000854814.1 NZ_CP021288:2169838-2170212 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T76420 NZ_CP021288:2169838-2170212 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13563.41 Da        Isoelectric Point: 5.0468

>AT76420 WP_001285586.1 NZ_CP021288:2169381-2169749 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT76420 NZ_CP021288:2169381-2169749 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGTTGCTGATGAAACAACTGGAGCTCATGCTCACCAGTGGT
GAACTCAGCCCCCGCTATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB S1PWQ3

References