Detailed information of TA system TA076399

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 111436..111964
• Replicon: 1 (D-7474) plasmid unnamed
• Accession: NZ_CP021287
• Organism: Legionella pneumophila subsp. pneumophila strain Albuquerque

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A0A8UYT5
• Locus tag: CA919_RS16465
• Protein ID: WP_011212544.1
• Coordinates: 111436..111723 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A0A8UU85
• Locus tag: CA919_RS16470
• Protein ID: WP_011212545.1
• Coordinates: 111713..111964 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA919_RS16435	106743..108251	+	1509	WP_011212538.1	DUF3987 domain-containing protein	-
CA919_RS16440	108302..108877	+	576	WP_011212539.1	pyridoxamine 5'-phosphate oxidase family protein	-
CA919_RS16445	108886..109197	+	312	WP_011212540.1	hypothetical protein	-
CA919_RS16450	109253..109435	+	183	WP_011212541.1	hypothetical protein	-
CA919_RS16455	109529..110404	-	876	WP_011212542.1	alpha/beta hydrolase	-
CA919_RS16460	110462..111223	-	762	WP_011212543.1	MerR family transcriptional regulator	-
CA919_RS16465	111436..111723	-	288	WP_011212544.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CA919_RS16470	111713..111964	-	252	WP_011212545.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
CA919_RS16475	112213..112680	-	468	WP_011212546.1	hypothetical protein	-
CA919_RS16480	113299..114120	-	822	WP_011212547.1	DUF4111 domain-containing protein	-
CA919_RS16485	114104..114541	-	438	WP_010655396.1	DNA-binding protein	-
CA919_RS16490	114528..115106	-	579	WP_010655397.1	nucleotidyltransferase domain-containing protein	-
CA919_RS16495	115394..116274	-	881	Protein_122	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaOXA-29	-	1..129880	129880

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (8-68)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11302.35 Da
• Isoelectric Point: 10.4380

>T76399 WP_011212544.1 NZ_CP021287:c111723-111436 [Legionella pneumophila subsp. pneumophila]
MIYELHFHPLALKEWKKLSKELQEQFKKLLKRRLENPHVISAKLSGHLKDCYKIKLHQAGYRLVYQVNDNKMILLVVAVG
KRNKNKVYESAEDRT

Nucleotide

• Length: 288 bp

>T76399 NZ_CP021287:c111723-111436 [Legionella pneumophila subsp. pneumophila]
ATGATCTATGAGTTACATTTCCATCCGCTTGCGTTAAAAGAATGGAAGAAACTATCGAAGGAGTTGCAGGAACAGTTTAA
AAAACTATTAAAACGGCGACTGGAAAATCCGCATGTTATTTCTGCCAAACTTAGTGGCCATCTTAAAGATTGCTATAAGA
TAAAATTGCATCAGGCCGGCTATAGATTGGTCTACCAAGTTAACGATAATAAAATGATTCTCCTTGTTGTTGCTGTAGGC
AAGCGAAACAAAAATAAAGTCTATGAGTCAGCAGAAGATAGGACTTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9165.58 Da
• Isoelectric Point: 4.5164

>AT76399 WP_011212545.1 NZ_CP021287:c111964-111713 [Legionella pneumophila subsp. pneumophila]
MQTLYATQVASISELKKNPTKLINDAHGMPVVILNHNVAAAYLIPAETFEKLMDAVDESELKSIVEQRLSEPFTPVKVAL
DDL

Nucleotide

• Length: 252 bp

>AT76399 NZ_CP021287:c111964-111713 [Legionella pneumophila subsp. pneumophila]
ATGCAAACTTTATATGCAACACAAGTCGCTAGCATTAGCGAATTAAAGAAAAATCCCACAAAACTTATTAATGATGCTCA
TGGGATGCCCGTGGTGATTCTTAACCATAATGTAGCTGCTGCTTATTTAATTCCAGCAGAAACTTTTGAAAAACTGATGG
ATGCTGTTGACGAAAGCGAGCTAAAAAGCATTGTAGAACAACGCCTTTCAGAGCCCTTTACGCCAGTTAAGGTTGCGCTT
GATGATCTATGA

Structures

Toxin

• AlphaFold DB: A0A0A8UYT5

Antitoxin

• AlphaFold DB: A0A0A8UU85