Detailed information of TA system TA076397

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 66832..68369
• Replicon: chromosome
• Accession: NZ_CP021286
• Organism: Legionella pneumophila subsp. pneumophila strain Albuquerque

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: CA919_RS00340
• Protein ID: WP_041173955.1
• Coordinates: 66832..68130 (-)
• Length: 433 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A3A6W1F4
• Locus tag: CA919_RS00345
• Protein ID: WP_011212708.1
• Coordinates: 68130..68369 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA919_RS00310	62313..63032	+	720	WP_011212701.1	C39 family peptidase	-
CA919_RS00315	63068..63628	+	561	WP_011212702.1	hypothetical protein	-
CA919_RS16605	63736..63897	+	162	WP_015444967.1	hypothetical protein	-
CA919_RS00320	63934..64338	-	405	WP_011212703.1	MerR family transcriptional regulator	-
CA919_RS00325	64408..65151	+	744	WP_011212704.1	glutaredoxin family protein	-
CA919_RS00330	65350..66441	+	1092	WP_011212705.1	hypothetical protein	-
CA919_RS00335	66483..66801	+	319	Protein_63	integrase	-
CA919_RS00340	66832..68130	-	1299	WP_041173955.1	type II toxin-antitoxin system HipA family toxin	Toxin
CA919_RS00345	68130..68369	-	240	WP_011212708.1	helix-turn-helix domain-containing protein	Antitoxin
CA919_RS00350	68463..68693	+	231	Protein_66	hypothetical protein	-
CA919_RS00355	69127..69492	+	366	WP_011212709.1	TraK family protein	-
CA919_RS00360	69790..70635	-	846	WP_011212710.1	hypothetical protein	-
CA919_RS00365	70964..71218	-	255	WP_041173947.1	hypothetical protein	-
CA919_RS00370	71756..72697	+	942	WP_011212712.1	hypothetical protein	-
CA919_RS00375	72706..72801	+	96	Protein_71	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (3-104)

Antitoxin

• Cro/C1-type helix-turn-helix domain (15-65)

Sequences

Toxin

Protein

• Length: 433 a.a.
• Molecular weight: 49362.61 Da
• Isoelectric Point: 8.0440

>T76397 WP_041173955.1 NZ_CP021286:c68130-66832 [Legionella pneumophila subsp. pneumophila]
MRKLYILMNNELVGVLTQDNAGGLSFSYVADWISKPHSRPVSLSLPLINQRFSGEVVYNFFDNLLPDNPQIRARIQARFQ
AKSSRPFDLLALIGGDCVGAIQIVERIPQTQRLSIESHVVSEAEIARTLRSYQTSPLGMDKEDDEFRISIAGAQEKTAFL
YYQNEWHKPFGPTPTTHIFKLPIGYLPHQGIDLSESCENEHLCLLLASKFGLEVAKSKILQFEDEKVLVVERFDRKWSRD
NRYILRLPQEDLCQALGYSPNLKYQSDGGPGIKEIMQLLNGSQQPQKDRDEFFRAQVVYWCLGAIDAHAKNFSISIQPQG
GYQLTPLYDIISAYAIVEQKQLSFKKLKMAMALYGKNSHYKIDGIQKRHFIETAKYVQYSKIKAAQIFEQVIDRIDSAID
EVSTQLPNHFPEKIADPIFKGMRAFKERMIST

Nucleotide

• Length: 1299 bp

>T76397 NZ_CP021286:c68130-66832 [Legionella pneumophila subsp. pneumophila]
ATGCGTAAATTATACATATTGATGAACAATGAGCTCGTGGGGGTGCTTACCCAGGATAATGCAGGTGGTTTGAGTTTTTC
CTACGTTGCTGACTGGATTTCCAAGCCGCATTCACGCCCAGTTTCTTTATCGTTACCGTTAATAAATCAAAGGTTTTCAG
GAGAAGTTGTTTATAACTTTTTTGATAATTTACTTCCCGATAATCCACAGATAAGAGCGAGAATTCAAGCTCGTTTTCAG
GCAAAATCATCGCGACCGTTTGATTTATTGGCGTTAATTGGCGGTGATTGTGTTGGTGCAATACAAATTGTTGAACGTAT
TCCTCAGACACAGCGTCTAAGCATTGAAAGTCACGTTGTCAGTGAGGCCGAGATAGCTCGTACTTTACGTTCTTACCAAA
CCTCACCACTGGGAATGGATAAAGAGGATGATGAGTTTAGAATCTCAATAGCCGGTGCTCAGGAAAAAACGGCTTTTCTA
TATTACCAAAATGAATGGCATAAACCATTTGGACCTACACCAACAACTCACATATTCAAATTACCTATTGGATATTTACC
ACATCAAGGAATTGATTTAAGTGAGAGTTGTGAAAATGAGCATCTCTGTTTACTACTAGCCAGCAAATTTGGTTTAGAGG
TTGCAAAATCAAAAATCTTACAATTCGAAGATGAAAAGGTGCTGGTTGTCGAAAGATTTGATAGAAAATGGAGTCGAGAT
AACCGTTATATACTCAGGTTACCGCAAGAAGACCTATGCCAAGCGTTGGGTTATTCACCAAATTTAAAATATCAGTCAGA
TGGTGGCCCCGGTATTAAAGAAATTATGCAGCTACTTAATGGCTCACAGCAGCCTCAAAAAGATAGAGATGAATTTTTCA
GAGCACAGGTTGTTTACTGGTGCCTTGGTGCTATTGATGCGCATGCCAAAAACTTCAGTATAAGTATACAACCACAAGGC
GGTTACCAGCTCACCCCCTTATACGACATCATTTCAGCCTATGCTATCGTGGAACAAAAACAGCTATCCTTTAAGAAATT
GAAAATGGCAATGGCTTTATATGGTAAAAATAGTCATTACAAGATTGACGGTATTCAAAAAAGACACTTTATTGAAACAG
CAAAATACGTTCAATATTCTAAAATTAAAGCAGCTCAGATTTTTGAACAAGTGATTGACCGTATTGATTCTGCTATCGAT
GAGGTCTCAACACAATTACCAAACCACTTCCCAGAAAAGATTGCAGATCCAATCTTCAAAGGGATGAGAGCTTTTAAGGA
GCGAATGATATCCACATAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9082.37 Da
• Isoelectric Point: 5.0628

>AT76397 WP_011212708.1 NZ_CP021286:c68369-68130 [Legionella pneumophila subsp. pneumophila]
MIISSAKDLALYLNDKRKQLKMSQSEVADLVGLKQDTISKFENSPDNSRIDTLFRILSALNLNMSLVEKDQKTHEDIEW

Nucleotide

• Length: 240 bp

>AT76397 NZ_CP021286:c68369-68130 [Legionella pneumophila subsp. pneumophila]
ATGATCATTAGCTCAGCAAAAGATTTAGCATTATATTTAAATGACAAGCGCAAACAACTCAAAATGAGTCAATCTGAAGT
TGCCGATTTAGTCGGCTTAAAACAGGATACCATATCAAAGTTTGAAAATAGCCCTGATAATAGTCGAATTGATACCTTAT
TTAGAATATTGTCCGCCCTAAATTTAAATATGAGCCTTGTTGAAAAAGATCAAAAAACTCATGAAGATATAGAATGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A3A6W1F4