Detailed information of TA system TA076391

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 116268..116796
• Replicon: 2 (D-7477) plasmid unnamed
• Accession: NZ_CP021282
• Organism: Legionella pneumophila subsp. pneumophila strain Flint

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A0A8UYT5
• Locus tag: CA924_RS16960
• Protein ID: WP_011212544.1
• Coordinates: 116509..116796 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A0A8UU85
• Locus tag: CA924_RS16955
• Protein ID: WP_011212545.1
• Coordinates: 116268..116519 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA924_RS16930	111957..112838	+	882	WP_021436862.1	hypothetical protein	-
CA924_RS16935	113126..113704	+	579	WP_010655397.1	nucleotidyltransferase domain-containing protein	-
CA924_RS16940	113691..114128	+	438	WP_010655396.1	DNA-binding protein	-
CA924_RS16945	114112..114933	+	822	WP_011212547.1	DUF4111 domain-containing protein	-
CA924_RS16950	115552..116019	+	468	WP_011212546.1	hypothetical protein	-
CA924_RS16955	116268..116519	+	252	WP_011212545.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
CA924_RS16960	116509..116796	+	288	WP_011212544.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CA924_RS16965	117009..117770	+	762	WP_011212543.1	MerR family transcriptional regulator	-
CA924_RS16970	117828..118703	+	876	WP_011212542.1	alpha/beta hydrolase	-
CA924_RS16975	118797..118979	-	183	WP_011212541.1	hypothetical protein	-
CA924_RS16980	119035..119346	-	312	WP_011212540.1	hypothetical protein	-
CA924_RS16985	119355..119930	-	576	WP_011212539.1	pyridoxamine 5'-phosphate oxidase family protein	-
CA924_RS16990	119981..121489	-	1509	WP_011212538.1	DUF3987 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaOXA-29	-	1..129884	129884

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (8-68)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11302.35 Da
• Isoelectric Point: 10.4380

>T76391 WP_011212544.1 NZ_CP021282:116509-116796 [Legionella pneumophila subsp. pneumophila]
MIYELHFHPLALKEWKKLSKELQEQFKKLLKRRLENPHVISAKLSGHLKDCYKIKLHQAGYRLVYQVNDNKMILLVVAVG
KRNKNKVYESAEDRT

Nucleotide

• Length: 288 bp

>T76391 NZ_CP021282:116509-116796 [Legionella pneumophila subsp. pneumophila]
ATGATCTATGAGTTACATTTCCATCCGCTTGCGTTAAAAGAATGGAAGAAACTATCGAAGGAGTTGCAGGAACAGTTTAA
AAAACTATTAAAACGGCGACTGGAAAATCCGCATGTTATTTCTGCCAAACTTAGTGGCCATCTTAAAGATTGCTATAAGA
TAAAATTGCATCAGGCCGGCTATAGATTGGTCTACCAAGTTAACGATAATAAAATGATTCTCCTTGTTGTTGCTGTAGGC
AAGCGAAACAAAAATAAAGTCTATGAGTCAGCAGAAGATAGGACTTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9165.58 Da
• Isoelectric Point: 4.5164

>AT76391 WP_011212545.1 NZ_CP021282:116268-116519 [Legionella pneumophila subsp. pneumophila]
MQTLYATQVASISELKKNPTKLINDAHGMPVVILNHNVAAAYLIPAETFEKLMDAVDESELKSIVEQRLSEPFTPVKVAL
DDL

Nucleotide

• Length: 252 bp

>AT76391 NZ_CP021282:116268-116519 [Legionella pneumophila subsp. pneumophila]
ATGCAAACTTTATATGCAACACAAGTCGCTAGCATTAGCGAATTAAAGAAAAATCCCACAAAACTTATTAATGATGCTCA
TGGGATGCCCGTGGTGATTCTTAACCATAATGTAGCTGCTGCTTATTTAATTCCAGCAGAAACTTTTGAAAAACTGATGG
ATGCTGTTGACGAAAGCGAGCTAAAAAGCATTGTAGAACAACGCCTTTCAGAGCCCTTTACGCCAGTTAAGGTTGCGCTT
GATGATCTATGA

Structures

Toxin

• AlphaFold DB: A0A0A8UYT5

Antitoxin

• AlphaFold DB: A0A0A8UU85