Detailed information of TA system TA076390

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 2322290..2324011
• Replicon: chromosome
• Accession: NZ_CP021281
• Organism: Legionella pneumophila subsp. pneumophila strain Flint

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: -
• Locus tag: CA924_RS10605
• Protein ID: WP_038839099.1
• Coordinates: 2322290..2323222 (-)
• Length: 311 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: A0A128I8L5
• Locus tag: CA924_RS10610
• Protein ID: WP_015444253.1
• Coordinates: 2323226..2324011 (-)
• Length: 262 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA924_RS10580	2317849..2318202	-	354	WP_015444259.1	conjugal transfer transcriptional regulator TraJ	-
CA924_RS10585	2318596..2318940	+	345	WP_015444258.1	TraK family protein	-
CA924_RS10590	2318940..2319665	+	726	WP_015444257.1	IncP-type DNA transfer protein TraL	-
CA924_RS10595	2319665..2320102	+	438	WP_015444256.1	conjugal transfer protein TraM	-
CA924_RS10600	2320217..2322157	+	1941	WP_038839098.1	DUF2779 domain-containing protein	-
CA924_RS10605	2322290..2323222	-	933	WP_038839099.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
CA924_RS10610	2323226..2324011	-	786	WP_015444253.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
CA924_RS10615	2324496..2325500	-	1005	WP_010947770.1	site-specific integrase	-
CA924_RS10620	2325413..2325715	-	303	WP_010947771.1	helix-turn-helix domain-containing protein	-
CA924_RS10630	2326125..2326748	+	624	WP_010947772.1	helix-turn-helix domain-containing protein	-
CA924_RS10635	2326745..2326951	-	207	WP_021436844.1	hypothetical protein	-
CA924_RS10640	2327112..2328419	+	1308	WP_010947773.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2248997..2388641	139644

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (13-264)

Antitoxin

• Transcriptional regulator, AbiEi 3 antitoxin, Type IV TA system (97-254)

Sequences

Toxin

Protein

• Length: 311 a.a.
• Molecular weight: 35330.14 Da
• Isoelectric Point: 6.8643

>T76390 WP_038839099.1 NZ_CP021281:c2323222-2322290 [Legionella pneumophila subsp. pneumophila]
MNKQSPYYKQVLLLIRLIPLVEKETCFALKGGTAINLFVRDFPRLSVDIDLAYLPLEPREIALANVRNALAGIAANINKI
PDLNAVLQDNKPDEMRIIVEDHSSLNRGTQIKIEVSPVARGTLLPPVELDVVASVENEFGFASMKVVSLPDLYGGKLCAA
LDRQHPRDLFDVKVLLENQKIDRELFNGFLTYLLSHKRPISEIMNPRWKDITQVFEREFKGMTAEPVTLEELTAIPGKMI
QGLKDNFTQQDYDFLVSFKRGEPEWSLACSDQIQYLPAVQWKLLNIRKMPKQKLMDSVSLLQRTMGHWLN

Nucleotide

• Length: 933 bp

>T76390 NZ_CP021281:c2323222-2322290 [Legionella pneumophila subsp. pneumophila]
ATGAATAAACAGTCACCATATTATAAACAGGTTTTGTTGCTCATTCGTTTAATTCCACTCGTCGAAAAGGAAACTTGTTT
TGCTTTAAAGGGAGGTACTGCCATTAATTTATTTGTGCGAGATTTCCCGAGGTTATCTGTGGATATAGATCTAGCGTATT
TACCCCTCGAGCCACGTGAGATTGCTTTAGCCAATGTGCGTAATGCATTAGCGGGTATTGCGGCAAATATTAATAAAATT
CCAGATTTGAATGCAGTTTTGCAAGATAACAAACCAGATGAAATGCGAATTATTGTTGAAGATCATTCTTCTCTAAATAG
GGGGACCCAAATCAAGATAGAAGTTTCACCTGTTGCGCGTGGAACTCTTTTGCCGCCAGTTGAGCTTGATGTTGTTGCTT
CGGTGGAAAATGAGTTTGGTTTTGCGTCAATGAAAGTCGTTTCTTTGCCTGATCTCTATGGTGGTAAATTATGCGCTGCT
TTAGATCGCCAGCATCCTAGAGATCTTTTTGATGTTAAGGTTTTGTTAGAAAATCAAAAGATTGACCGAGAGCTTTTTAA
TGGGTTTCTTACTTATCTTTTAAGTCACAAAAGGCCTATTTCAGAAATAATGAATCCACGATGGAAAGATATAACTCAAG
TTTTTGAACGTGAATTTAAAGGAATGACTGCTGAACCAGTTACCCTGGAAGAGCTAACAGCAATACCAGGAAAAATGATT
CAGGGTCTTAAAGATAATTTTACTCAGCAGGATTATGATTTTTTAGTCTCTTTTAAAAGGGGCGAGCCTGAGTGGTCTCT
TGCATGTAGCGATCAGATTCAATATCTCCCAGCAGTACAATGGAAGCTTTTAAATATTCGCAAAATGCCCAAACAAAAAC
TCATGGATTCAGTTAGCTTGCTACAAAGAACGATGGGACATTGGCTTAATTAG

Antitoxin

Protein

• Length: 262 a.a.
• Molecular weight: 29773.22 Da
• Isoelectric Point: 9.4457

>AT76390 WP_015444253.1 NZ_CP021281:c2324011-2323226 [Legionella pneumophila subsp. pneumophila]
MSSKINWLLNNIASGEILLQSWLSSHGITPQLARKYCQSQWLVKLGVGVYYRAGRKPRWQNAVYCLQNQSRYMIHLAGLT
SLAQQGKSHYLQFAPDSIWINVQAKTILPKWFKNFPELVISDELIPNWEIIKTSKLNAQDENDLTTIEVDGIKLKASNQE
LAAFEVLEAVPSTLSFEHAAELFQGLVNLSPRKVEHILKRSGSIQTNRLFLFLGHFYKHPWAERVNESLINLGAGKRQIV
PDGKLEKQYQITVPEQFISSD

Nucleotide

• Length: 786 bp

>AT76390 NZ_CP021281:c2324011-2323226 [Legionella pneumophila subsp. pneumophila]
ATGTCATCAAAAATAAACTGGCTTCTGAATAATATTGCCTCAGGTGAAATCTTGCTCCAGTCTTGGTTATCAAGCCATGG
AATTACTCCACAATTAGCAAGAAAATATTGTCAAAGCCAATGGTTGGTAAAATTGGGGGTAGGGGTTTATTATCGTGCAG
GGCGTAAACCTAGATGGCAAAATGCAGTTTATTGTCTTCAGAATCAGAGTCGGTACATGATTCATCTTGCTGGTTTAACC
AGTTTGGCTCAGCAGGGCAAATCGCATTACCTACAGTTTGCACCAGACTCTATTTGGATAAACGTTCAAGCAAAAACGAT
TTTACCAAAGTGGTTTAAAAATTTTCCAGAATTAGTTATTTCTGATGAACTAATACCAAATTGGGAAATCATTAAAACAT
CCAAGTTAAATGCTCAGGATGAGAATGACTTAACTACTATAGAAGTAGATGGGATTAAGTTAAAAGCAAGTAATCAAGAG
TTAGCCGCATTCGAGGTGCTGGAGGCTGTACCTTCCACTTTGTCATTTGAGCATGCAGCAGAGCTTTTTCAAGGATTGGT
AAACTTAAGTCCACGAAAAGTAGAACATATTTTAAAAAGAAGCGGATCTATTCAAACGAACCGACTTTTTTTATTTTTGG
GTCATTTCTATAAACATCCATGGGCTGAACGGGTGAATGAGTCCTTAATTAATCTTGGTGCAGGTAAGCGTCAAATAGTC
CCTGATGGAAAATTAGAAAAACAATATCAAATCACCGTTCCAGAACAATTTATAAGTAGCGATTAA

Structures

Antitoxin

• AlphaFold DB: A0A128I8L5