Detailed information of TA system TA076388

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 66834..68371
• Replicon: chromosome
• Accession: NZ_CP021281
• Organism: Legionella pneumophila subsp. pneumophila strain Flint

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: CA924_RS00340
• Protein ID: WP_041173955.1
• Coordinates: 66834..68132 (-)
• Length: 433 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A3A6W1F4
• Locus tag: CA924_RS00345
• Protein ID: WP_011212708.1
• Coordinates: 68132..68371 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA924_RS00310	62315..63034	+	720	WP_011212701.1	C39 family peptidase	-
CA924_RS00315	63070..63630	+	561	WP_011212702.1	hypothetical protein	-
CA924_RS17075	63738..63899	+	162	WP_015444967.1	hypothetical protein	-
CA924_RS00320	63936..64340	-	405	WP_011212703.1	MerR family transcriptional regulator	-
CA924_RS00325	64410..65153	+	744	WP_011212704.1	glutaredoxin family protein	-
CA924_RS00330	65352..66443	+	1092	WP_011212705.1	hypothetical protein	-
CA924_RS00335	66485..66803	+	319	Protein_63	integrase	-
CA924_RS00340	66834..68132	-	1299	WP_041173955.1	type II toxin-antitoxin system HipA family toxin	Toxin
CA924_RS00345	68132..68371	-	240	WP_011212708.1	helix-turn-helix domain-containing protein	Antitoxin
CA924_RS00350	68465..68695	+	231	Protein_66	hypothetical protein	-
CA924_RS00355	69129..69494	+	366	WP_011212709.1	TraK family protein	-
CA924_RS00360	69792..70637	-	846	WP_011212710.1	hypothetical protein	-
CA924_RS00365	70966..71220	-	255	WP_041173947.1	hypothetical protein	-
CA924_RS00370	71758..72699	+	942	WP_011212712.1	hypothetical protein	-
CA924_RS00375	72708..72803	+	96	Protein_71	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (3-104)

Antitoxin

• Cro/C1-type helix-turn-helix domain (15-65)

Sequences

Toxin

Protein

• Length: 433 a.a.
• Molecular weight: 49362.61 Da
• Isoelectric Point: 8.0440

>T76388 WP_041173955.1 NZ_CP021281:c68132-66834 [Legionella pneumophila subsp. pneumophila]
MRKLYILMNNELVGVLTQDNAGGLSFSYVADWISKPHSRPVSLSLPLINQRFSGEVVYNFFDNLLPDNPQIRARIQARFQ
AKSSRPFDLLALIGGDCVGAIQIVERIPQTQRLSIESHVVSEAEIARTLRSYQTSPLGMDKEDDEFRISIAGAQEKTAFL
YYQNEWHKPFGPTPTTHIFKLPIGYLPHQGIDLSESCENEHLCLLLASKFGLEVAKSKILQFEDEKVLVVERFDRKWSRD
NRYILRLPQEDLCQALGYSPNLKYQSDGGPGIKEIMQLLNGSQQPQKDRDEFFRAQVVYWCLGAIDAHAKNFSISIQPQG
GYQLTPLYDIISAYAIVEQKQLSFKKLKMAMALYGKNSHYKIDGIQKRHFIETAKYVQYSKIKAAQIFEQVIDRIDSAID
EVSTQLPNHFPEKIADPIFKGMRAFKERMIST

Nucleotide

• Length: 1299 bp

>T76388 NZ_CP021281:c68132-66834 [Legionella pneumophila subsp. pneumophila]
ATGCGTAAATTATACATATTGATGAACAATGAGCTCGTGGGGGTGCTTACCCAGGATAATGCAGGTGGTTTGAGTTTTTC
CTACGTTGCTGACTGGATTTCCAAGCCGCATTCACGCCCAGTTTCTTTATCGTTACCGTTAATAAATCAAAGGTTTTCAG
GAGAAGTTGTTTATAACTTTTTTGATAATTTACTTCCCGATAATCCACAGATAAGAGCGAGAATTCAAGCTCGTTTTCAG
GCAAAATCATCGCGACCGTTTGATTTATTGGCGTTAATTGGCGGTGATTGTGTTGGTGCAATACAAATTGTTGAACGTAT
TCCTCAGACACAGCGTCTAAGCATTGAAAGTCACGTTGTCAGTGAGGCCGAGATAGCTCGTACTTTACGTTCTTACCAAA
CCTCACCACTGGGAATGGATAAAGAGGATGATGAGTTTAGAATCTCAATAGCCGGTGCTCAGGAAAAAACGGCTTTTCTA
TATTACCAAAATGAATGGCATAAACCATTTGGACCTACACCAACAACTCACATATTCAAATTACCTATTGGATATTTACC
ACATCAAGGAATTGATTTAAGTGAGAGTTGTGAAAATGAGCATCTCTGTTTACTACTAGCCAGCAAATTTGGTTTAGAGG
TTGCAAAATCAAAAATCTTACAATTCGAAGATGAAAAGGTGCTGGTTGTCGAAAGATTTGATAGAAAATGGAGTCGAGAT
AACCGTTATATACTCAGGTTACCGCAAGAAGACCTATGCCAAGCGTTGGGTTATTCACCAAATTTAAAATATCAGTCAGA
TGGTGGCCCCGGTATTAAAGAAATTATGCAGCTACTTAATGGCTCACAGCAGCCTCAAAAAGATAGAGATGAATTTTTCA
GAGCACAGGTTGTTTACTGGTGCCTTGGTGCTATTGATGCGCATGCCAAAAACTTCAGTATAAGTATACAACCACAAGGC
GGTTACCAGCTCACCCCCTTATACGACATCATTTCAGCCTATGCTATCGTGGAACAAAAACAGCTATCCTTTAAGAAATT
GAAAATGGCAATGGCTTTATATGGTAAAAATAGTCATTACAAGATTGACGGTATTCAAAAAAGACACTTTATTGAAACAG
CAAAATACGTTCAATATTCTAAAATTAAAGCAGCTCAGATTTTTGAACAAGTGATTGACCGTATTGATTCTGCTATCGAT
GAGGTCTCAACACAATTACCAAACCACTTCCCAGAAAAGATTGCAGATCCAATCTTCAAAGGGATGAGAGCTTTTAAGGA
GCGAATGATATCCACATAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9082.37 Da
• Isoelectric Point: 5.0628

>AT76388 WP_011212708.1 NZ_CP021281:c68371-68132 [Legionella pneumophila subsp. pneumophila]
MIISSAKDLALYLNDKRKQLKMSQSEVADLVGLKQDTISKFENSPDNSRIDTLFRILSALNLNMSLVEKDQKTHEDIEW

Nucleotide

• Length: 240 bp

>AT76388 NZ_CP021281:c68371-68132 [Legionella pneumophila subsp. pneumophila]
ATGATCATTAGCTCAGCAAAAGATTTAGCATTATATTTAAATGACAAGCGCAAACAACTCAAAATGAGTCAATCTGAAGT
TGCCGATTTAGTCGGCTTAAAACAGGATACCATATCAAAGTTTGAAAATAGCCCTGATAATAGTCGAATTGATACCTTAT
TTAGAATATTGTCCGCCCTAAATTTAAATATGAGCCTTGTTGAAAAAGATCAAAAAACTCATGAAGATATAGAATGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A3A6W1F4