Detailed information of TA system TA076387

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafQ-RelB
• Location: 4951..5485
• Replicon: plasmid unnamed
• Accession: NZ_CP021280
• Organism: Legionella pneumophila subsp. fraseri strain F-4198

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: CA927_RS15645
• Protein ID: WP_106314980.1
• Coordinates: 5204..5485 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A433K571
• Locus tag: CA927_RS15640
• Protein ID: WP_062723413.1
• Coordinates: 4951..5211 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA927_RS15625	696..1268	-	573	WP_106314978.1	recombinase family protein	-
CA927_RS15630	1403..4408	+	3006	WP_106314979.1	Tn3 family transposase	-
CA927_RS15635	4476..4733	+	258	Protein_3	site-specific integrase	-
CA927_RS15640	4951..5211	+	261	WP_062723413.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
CA927_RS15645	5204..5485	+	282	WP_106314980.1	type II toxin-antitoxin system YafQ family toxin	Toxin
CA927_RS15650	5537..5866	-	330	WP_106314981.1	hypothetical protein	-
CA927_RS15655	5955..7448	-	1494	WP_106315053.1	DUF3987 domain-containing protein	-
CA927_RS15660	7454..8353	-	900	WP_106314982.1	Abi family protein	-
CA927_RS15665	8606..9241	+	636	WP_106314983.1	hypothetical protein	-
CA927_RS15670	9245..10081	-	837	WP_106314984.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..88504	88504

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-92)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10928.62 Da
• Isoelectric Point: 8.9819

>T76387 WP_106314980.1 NZ_CP021280:5204-5485 [Legionella pneumophila subsp. fraseri]
MLELELATQFKRDLQKITKQGKSRKILDSIVEQLQKEEPLDLKHKDHSLTGNWGGYRECHLTPDWLLIYKIIKDDRIQLL
RLSRTGSHSDLFG

Nucleotide

• Length: 282 bp

>T76387 NZ_CP021280:5204-5485 [Legionella pneumophila subsp. fraseri]
ATGCTTGAATTAGAATTGGCGACACAATTTAAGCGTGATTTGCAAAAGATTACCAAACAAGGGAAAAGCAGGAAGATTCT
GGACTCCATTGTTGAGCAATTGCAAAAGGAGGAGCCTTTGGATCTGAAACATAAGGATCATAGTCTTACCGGAAATTGGG
GTGGTTATCGTGAATGTCACCTTACCCCCGATTGGTTATTAATTTATAAGATTATTAAAGATGATCGCATACAATTGTTA
CGATTATCGCGAACAGGCTCTCATAGCGATTTGTTTGGATGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9553.02 Da
• Isoelectric Point: 6.9797

>AT76387 WP_062723413.1 NZ_CP021280:4951-5211 [Legionella pneumophila subsp. fraseri]
MSKAATINARIEPALKMQAEAILHKVGLSTAEAIRLFYSQVCLQNGLPFEVKIPNKETRDAMAELESGKGERFKTMKDVW
DSVDNA

Nucleotide

• Length: 261 bp

>AT76387 NZ_CP021280:4951-5211 [Legionella pneumophila subsp. fraseri]
ATGAGTAAGGCGGCAACAATTAATGCTCGTATTGAGCCTGCTCTTAAAATGCAGGCCGAGGCGATTTTGCATAAGGTGGG
TTTATCTACAGCCGAGGCAATTCGATTGTTCTATAGTCAGGTCTGTCTTCAAAATGGCCTGCCGTTTGAGGTAAAAATAC
CGAATAAAGAGACCCGTGATGCTATGGCAGAACTGGAATCAGGTAAGGGCGAGCGATTTAAAACAATGAAGGATGTATGG
GATTCTGTAGATAATGCTTGA

Structures

Antitoxin

• AlphaFold DB: A0A433K571