Detailed information of TA system TA076385

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 2644412..2645846
• Replicon: chromosome
• Accession: NZ_CP021279
• Organism: Legionella pneumophila subsp. fraseri strain F-4198

Toxin (Protein)

• Gene name: HipA1
• Uniprot ID: A0A2S6EXC5
• Locus tag: CA927_RS11820
• Protein ID: WP_032830191.1
• Coordinates: 2644626..2645846 (+)
• Length: 407 a.a.

Antitoxin (Protein)

• Gene name: HipB1
• Uniprot ID: -
• Locus tag: CA927_RS11815
• Protein ID: WP_027227407.1
• Coordinates: 2644412..2644639 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA927_RS11790	2640207..2641073	-	867	WP_027227403.1	transposase	-
CA927_RS11795	2641135..2641323	-	189	WP_071974648.1	helix-turn-helix domain-containing protein	-
CA927_RS11800	2641512..2642300	+	789	WP_027227404.1	hypothetical protein	-
CA927_RS11805	2642365..2642775	-	411	Protein_2288	transposase family protein	-
CA927_RS11810	2642894..2643916	-	1023	WP_027227406.1	hypothetical protein	-
CA927_RS11815	2644412..2644639	+	228	WP_027227407.1	helix-turn-helix transcriptional regulator	Antitoxin
CA927_RS11820	2644626..2645846	+	1221	WP_032830191.1	type II toxin-antitoxin system HipA family toxin	Toxin
CA927_RS11825	2645881..2646237	-	357	WP_027227408.1	preprotein translocase	-
CA927_RS11830	2646277..2647182	-	906	WP_027227409.1	LysR family transcriptional regulator	-
CA927_RS11835	2647273..2648133	+	861	WP_027227410.1	DUF2608 domain-containing protein	-
CA927_RS11840	2648485..2648749	-	265	Protein_2295	hypothetical protein	-
CA927_RS11845	2648797..2649108	-	312	WP_027227411.1	cupin	-
CA927_RS11850	2649124..2649372	-	249	WP_031301595.1	hypothetical protein	-
CA927_RS11855	2649679..2650152	+	474	WP_027227413.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (147-380)

Antitoxin

• Transcription regulator, HipB-like (7-63)

Sequences

Toxin

Protein

• Length: 407 a.a.
• Molecular weight: 45374.54 Da
• Isoelectric Point: 8.3174

>T76385 WP_032830191.1 NZ_CP021279:2644626-2645846 [Legionella pneumophila subsp. fraseri]
MKNHKLDIYLNQTLAGQLRIDTHGEMSFVYDEGYLENNKNLPLSHSLPLQQAPYSTKQCRPFFSGLLPEAHMRTAIARQL
GISEKNDFALLAAIGGECAGAVMILPQDTSITQLKPDYKIIKKQDVLEILQTMHQRPMIVGEDGIRLSLAGAQDKLPVAI
IEGNLAIPMNGAPSTHILKPINRDFPSLIENESFCLNLAQKIGLNAVDASIHYADGTPYLLVKRYDRVETKQGIQRLHQE
DFCQAMGISPEMKYQREGGPQISECFGLIREISSLPVLDIKELLQGILFNVIIGNNDAHSKNFSLLYHGSKVRLAPFYDL
ISTVYYPNLASKMAMKVGSKYDFDGLFPRHFIQMAEEARLSGALVRKEVLNLISKIQKNITASPFTSIILQRADKLFQKF
QKASTG

Nucleotide

• Length: 1221 bp

>T76385 NZ_CP021279:2644626-2645846 [Legionella pneumophila subsp. fraseri]
ATGAAAAATCATAAACTTGATATCTATCTTAACCAAACACTAGCAGGCCAATTACGTATCGATACGCATGGCGAGATGTC
TTTTGTGTATGATGAAGGCTACCTGGAAAACAATAAAAATCTCCCGTTGTCACACTCCCTTCCCCTTCAACAAGCCCCTT
ACTCAACGAAGCAATGCCGTCCGTTTTTTAGCGGACTATTGCCTGAAGCCCATATGCGTACTGCTATTGCCCGTCAATTG
GGTATCAGTGAAAAAAATGATTTTGCCTTATTAGCAGCTATTGGTGGTGAGTGTGCCGGAGCTGTGATGATATTACCTCA
AGATACATCCATAACTCAGCTAAAGCCAGACTATAAAATTATCAAAAAACAGGATGTATTAGAAATCCTGCAAACCATGC
ATCAAAGACCCATGATAGTCGGTGAGGATGGAATTCGTTTATCCCTTGCTGGAGCTCAGGATAAACTTCCAGTTGCCATT
ATTGAAGGAAATCTCGCAATACCAATGAACGGTGCACCGAGTACTCATATTTTAAAACCAATTAATCGAGACTTTCCCTC
CTTAATTGAAAATGAATCTTTCTGCCTTAATCTGGCACAAAAAATTGGATTAAATGCTGTGGACGCATCAATCCATTATG
CTGATGGTACCCCTTATCTGTTGGTTAAACGTTATGATCGGGTTGAGACAAAGCAAGGAATCCAACGTCTTCATCAAGAA
GATTTTTGTCAGGCAATGGGCATTAGCCCTGAAATGAAATATCAGCGAGAAGGCGGCCCCCAAATTAGTGAGTGCTTTGG
TTTGATAAGAGAGATATCAAGCCTCCCTGTCCTTGACATAAAAGAATTATTACAAGGGATTCTGTTTAATGTCATTATTG
GCAACAATGATGCACATAGCAAAAACTTCTCTTTGCTCTATCATGGTTCAAAAGTTCGTTTAGCACCTTTCTACGATCTG
ATTTCAACCGTATATTATCCTAATCTTGCCAGTAAAATGGCGATGAAAGTTGGAAGTAAATATGATTTTGATGGCCTATT
TCCACGCCACTTTATACAAATGGCAGAGGAGGCTCGTTTATCGGGTGCCTTAGTGCGTAAAGAAGTCTTGAATCTAATTA
GCAAAATTCAAAAGAACATAACCGCCAGCCCCTTTACCAGCATCATTTTGCAACGTGCTGACAAATTATTTCAAAAGTTC
CAGAAGGCTTCAACAGGATAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8467.85 Da
• Isoelectric Point: 9.1200

>AT76385 WP_027227407.1 NZ_CP021279:2644412-2644639 [Legionella pneumophila subsp. fraseri]
MIQSPENIGRYIRKVRKELNVTQKDLALTAGTGLRFIIDLENGKPTCQIGKMLQVLQVLGIQLNLSHTDFENEKS

Nucleotide

• Length: 228 bp

>AT76385 NZ_CP021279:2644412-2644639 [Legionella pneumophila subsp. fraseri]
ATGATTCAATCTCCAGAAAATATAGGACGTTATATTCGCAAAGTTCGTAAAGAACTTAATGTTACCCAAAAAGATTTGGC
TTTAACTGCTGGAACTGGATTGCGTTTTATTATCGATTTGGAAAATGGAAAGCCCACTTGCCAAATTGGAAAAATGCTGC
AAGTACTACAAGTACTTGGGATTCAACTCAACTTATCTCATACTGATTTCGAAAATGAAAAATCATAA

Structures

Toxin

• AlphaFold DB: A0A2S6EXC5