Detailed information of TA system TA076381

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 63470..64068
• Replicon: plasmid unnamed2
• Accession: NZ_CP021276
• Organism: Legionella pneumophila strain D-4040

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: CA938_RS16580
• Protein ID: WP_058475085.1
• Coordinates: 63871..64068 (-)
• Length: 66 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A378P8P3
• Locus tag: CA938_RS16575
• Protein ID: WP_058475084.1
• Coordinates: 63470..63874 (-)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA938_RS16550	58886..59209	+	324	WP_061363910.1	hypothetical protein	-
CA938_RS16695	59550..59681	-	132	WP_129462638.1	(2Fe-2S)-binding protein	-
CA938_RS16700	59718..60080	+	363	WP_129462639.1	hypothetical protein	-
CA938_RS16560	60119..60472	+	354	WP_061363912.1	type II toxin-antitoxin system RelE/ParE family toxin	-
CA938_RS16565	60450..60740	+	291	WP_021460513.1	helix-turn-helix domain-containing protein	-
CA938_RS16570	61769..63445	-	1677	WP_106195586.1	hypothetical protein	-
CA938_RS16575	63470..63874	-	405	WP_058475084.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CA938_RS16580	63871..64068	-	198	WP_058475085.1	type II toxin-antitoxin system HicA family toxin	Toxin
CA938_RS16585	64251..65135	+	885	WP_061363914.1	recombinase family protein	-
CA938_RS16590	65135..65596	+	462	WP_061363915.1	WGR domain-containing protein	-
CA938_RS16595	65614..66978	-	1365	WP_061363916.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..76951	76951

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-126)

Sequences

Toxin

Protein

• Length: 66 a.a.
• Molecular weight: 7389.76 Da
• Isoelectric Point: 10.7302

>T76381 WP_058475085.1 NZ_CP021276:c64068-63871 [Legionella pneumophila]
MKSAEIIKIIERDGWVKCHQKGSHCQFKHPTKKGRVTVPHPKKDLPVKTVASIFKQAGINKEDIK

Nucleotide

• Length: 198 bp

>T76381 NZ_CP021276:c64068-63871 [Legionella pneumophila]
ATGAAAAGCGCAGAAATAATAAAAATAATAGAACGCGATGGTTGGGTGAAGTGTCACCAGAAAGGGAGTCATTGCCAATT
TAAGCATCCAACAAAAAAGGGTAGGGTGACTGTGCCTCATCCTAAAAAAGATTTACCCGTTAAAACGGTAGCTAGTATTT
TTAAACAGGCTGGAATTAATAAGGAGGATATAAAATGA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14961.80 Da
• Isoelectric Point: 4.5209

>AT76381 WP_058475084.1 NZ_CP021276:c63874-63470 [Legionella pneumophila]
MRYPVVIHKDEHSDYGVSVPDIPGCYSAGATYDEALTNVIEAIECHLEGLLMDNESIPVGSTIDHWINEEEFQGGVWAFI
DIDLSQISGKSKRINITIPERVLNLIDLYSKNHAVKNRSSFLADAALSYIESHK

Nucleotide

• Length: 405 bp

>AT76381 NZ_CP021276:c63874-63470 [Legionella pneumophila]
ATGAGATATCCTGTAGTAATACATAAAGACGAGCACAGTGATTATGGCGTAAGTGTCCCAGATATTCCTGGATGTTATTC
TGCTGGAGCTACTTATGATGAGGCGTTAACTAACGTCATTGAGGCAATTGAATGTCATTTAGAAGGGTTGTTAATGGACA
ATGAGTCCATACCAGTAGGTTCTACAATTGATCATTGGATAAATGAGGAAGAGTTTCAGGGTGGAGTGTGGGCATTTATT
GATATTGATTTAAGTCAAATATCAGGTAAATCAAAACGCATCAATATCACTATTCCAGAGCGCGTATTAAATCTTATTGA
TTTATATTCTAAAAATCATGCAGTAAAAAATCGCTCTTCATTTTTAGCAGATGCAGCACTTAGTTATATAGAAAGTCATA
AATAA

Structures

Antitoxin

• AlphaFold DB: A0A378P8P3