Detailed information of TA system TA076370

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 2705984..2707446
• Replicon: chromosome
• Accession: NZ_CP021268
• Organism: Legionella pneumophila subsp. pneumophila strain Birmingham

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: -
• Locus tag: CA922_RS12330
• Protein ID: WP_042754790.1
• Coordinates: 2705984..2706841 (-)
• Length: 286 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: -
• Locus tag: CA922_RS12335
• Protein ID: WP_042754807.1
• Coordinates: 2706838..2707446 (-)
• Length: 203 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA922_RS12315	2702506..2702964	-	459	WP_042754792.1	hypothetical protein	-
CA922_RS12320	2703432..2705183	-	1752	WP_042754791.1	DUF927 domain-containing protein	-
CA922_RS12325	2705266..2705541	-	276	WP_014844516.1	helix-turn-helix domain-containing protein	-
CA922_RS12330	2705984..2706841	-	858	WP_042754790.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
CA922_RS12335	2706838..2707446	-	609	WP_042754807.1	AbiEi antitoxin N-terminal domain-containing protein	Antitoxin
CA922_RS12340	2707502..2707714	-	213	WP_042754789.1	hypothetical protein	-
CA922_RS12345	2707832..2709064	-	1233	WP_042754788.1	integrase family protein	-
CA922_RS12350	2709394..2710569	-	1176	WP_042754787.1	ISL3-like element ISLpn11 family transposase	-
CA922_RS12355	2710656..2711459	-	804	Protein_2382	IS30 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2654449..2709269	54820

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (37-261)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (9-56)

Sequences

Toxin

Protein

• Length: 286 a.a.
• Molecular weight: 31786.75 Da
• Isoelectric Point: 5.8066

>T76370 WP_042754790.1 NZ_CP021268:c2706841-2705984 [Legionella pneumophila subsp. pneumophila]
MTKDISASIRGRLKKIAKAEGSKSDFIAVLTRYGLERLLYRIGISEYASQFLLKGALLFNLWYDMPHRPTKDIDLLGFGD
NDLAYIQQTFRKICSLSADDGINFLSETVEVSTIKKDGGYTGARVELFGELAKAKIKIQVDIGYGDVVTPGPIDANYPVL
LSDLPAPQIRTYPVYTVIAEKLHAIALLGMANSRLKDYLDLYVLLTQEQLDNQVLAQAIHATFIRRGMTVPEVLPVGLSD
EFANDPTRISIWNAFLSKNELEQRSLTEVIEVIISLIQTPFSLAR

Nucleotide

• Length: 858 bp

>T76370 NZ_CP021268:c2706841-2705984 [Legionella pneumophila subsp. pneumophila]
ATGACAAAAGATATAAGTGCTTCCATTCGAGGACGACTTAAAAAAATAGCAAAAGCTGAAGGATCAAAGTCAGATTTTAT
TGCTGTCTTAACCCGTTATGGTCTCGAGCGGCTGTTATACCGAATTGGAATATCTGAATACGCCAGTCAATTTTTACTAA
AAGGTGCTTTACTATTTAATCTTTGGTATGACATGCCGCACCGCCCAACCAAGGATATTGATTTATTAGGGTTTGGCGAT
AATGATCTGGCTTATATCCAGCAAACGTTTAGAAAAATTTGCAGTCTTTCAGCTGATGATGGGATAAATTTTCTTTCTGA
AACCGTTGAAGTAAGCACTATCAAAAAAGATGGCGGTTATACTGGAGCAAGAGTTGAATTATTTGGTGAATTAGCAAAAG
CAAAGATCAAAATACAAGTTGATATTGGTTATGGAGATGTAGTAACTCCTGGTCCAATAGACGCTAATTACCCTGTACTT
TTAAGCGATCTTCCTGCTCCACAAATTCGTACTTATCCCGTTTATACTGTTATCGCAGAAAAATTGCACGCAATTGCATT
ATTGGGTATGGCTAATAGTCGATTGAAAGATTACTTAGACCTTTATGTGTTATTAACTCAAGAACAATTGGATAATCAAG
TTTTAGCCCAAGCTATACATGCCACATTTATTCGTCGGGGCATGACTGTACCTGAAGTACTACCTGTAGGTCTGTCCGAT
GAATTCGCCAATGACCCAACTAGAATATCAATTTGGAACGCTTTTTTAAGCAAAAATGAATTGGAACAGAGATCATTAAC
AGAAGTTATAGAGGTGATCATAAGTTTAATTCAAACACCATTTTCTTTGGCAAGGTAA

Antitoxin

Protein

• Length: 203 a.a.
• Molecular weight: 22903.75 Da
• Isoelectric Point: 9.0319

>AT76370 WP_042754807.1 NZ_CP021268:c2707446-2706838 [Legionella pneumophila subsp. pneumophila]
MKTEINYKQKVLQLAKKSGIVKISDLSAKGITRATVSRLVSENKLEKLAPGLYCLPEAEFSEKESLVIIASRVPQAVFCL
LTALQIHELTTQLPRKVWIAMPRSSHTPHIEYPPVKMVQYSEEAFSEGVEIIESDNTKLRVYNVAKTIADCFKHRNKIGL
DVAIEALKEAYTQNKVSMDDLWHYAKICRVANVMRPYLEAIQ

Nucleotide

• Length: 609 bp

>AT76370 NZ_CP021268:c2707446-2706838 [Legionella pneumophila subsp. pneumophila]
ATGAAGACAGAAATCAACTACAAACAAAAGGTACTCCAGTTGGCTAAAAAATCGGGGATTGTTAAAATTTCCGATCTAAG
CGCAAAGGGGATAACTAGAGCGACGGTTTCAAGGCTTGTTAGCGAAAACAAACTTGAAAAACTAGCTCCTGGACTCTATT
GCTTGCCTGAAGCTGAATTTTCAGAAAAAGAAAGTCTAGTTATTATAGCAAGTCGAGTTCCACAAGCAGTGTTTTGTTTG
CTTACTGCCTTGCAAATTCATGAGCTAACGACTCAGCTACCTCGAAAAGTATGGATAGCTATGCCAAGAAGCAGTCATAC
GCCGCACATAGAATATCCGCCAGTTAAAATGGTGCAATACTCAGAAGAAGCCTTTAGTGAAGGAGTTGAGATTATTGAGT
CCGATAATACGAAACTCAGAGTGTATAATGTAGCCAAGACTATTGCAGATTGTTTCAAACATCGCAACAAAATTGGCCTT
GATGTGGCAATTGAAGCACTTAAAGAAGCTTATACACAAAATAAAGTTTCAATGGATGATCTTTGGCATTATGCAAAAAT
ATGTCGAGTTGCTAATGTAATGCGTCCTTATCTAGAGGCTATCCAATGA