Detailed information of TA system TA076362

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 67637..69174
• Replicon: chromosome
• Accession: NZ_CP021266
• Organism: Legionella pneumophila subsp. pneumophila strain Knoxville

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: CA925_RS00325
• Protein ID: WP_032829405.1
• Coordinates: 67637..68941 (-)
• Length: 435 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: CA925_RS00330
• Protein ID: WP_014840730.1
• Coordinates: 68935..69174 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA925_RS00295	63122..63841	+	720	WP_014843028.1	C39 family peptidase	-
CA925_RS00300	63877..64437	+	561	WP_011212702.1	hypothetical protein	-
CA925_RS15540	64545..64706	+	162	WP_015444967.1	hypothetical protein	-
CA925_RS00305	64743..65147	-	405	WP_011212703.1	MerR family transcriptional regulator	-
CA925_RS00310	65217..65969	+	753	WP_027224671.1	glutaredoxin family protein	-
CA925_RS00315	66159..67250	+	1092	WP_027224672.1	hypothetical protein	-
CA925_RS00320	67292..67567	+	276	Protein_62	integrase	-
CA925_RS00325	67637..68941	-	1305	WP_032829405.1	type II toxin-antitoxin system HipA family toxin	Toxin
CA925_RS00330	68935..69174	-	240	WP_014840730.1	helix-turn-helix domain-containing protein	Antitoxin
CA925_RS00335	69541..69905	+	365	Protein_65	protein LvrA	-
CA925_RS00340	70015..70287	+	273	WP_032829407.1	hypothetical protein	-
CA925_RS15545	70353..70577	-	225	WP_027224674.1	hypothetical protein	-
CA925_RS00350	70982..72394	-	1413	WP_027224675.1	hypothetical protein	-
CA925_RS00355	72425..73033	-	609	WP_027224676.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (5-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 435 a.a.
• Molecular weight: 49704.13 Da
• Isoelectric Point: 8.2954

>T76362 WP_032829405.1 NZ_CP021266:c68941-67637 [Legionella pneumophila subsp. pneumophila]
MVMRKLYILMNNELVGELTQDNAGGLSFSYVADWISKPHSRPVSLSLPLVNQKFSGEVVYNFFDNLLPDNPQIRARIQAR
FQAKSSRPFDLLALIGSDCVGAIQIVERIPQTQRLSIESHVVSEAEIARTLRSYQTSPLGMDKEDDEFRISIAGAQEKTA
FLYYQNEWHKPFGPTPTTHIFKLPIGYLPHQGIDLSESCENEHLCLLLASKFGLDVAKSKILQFEDEKVLVVERFDRKWS
RDNRYILRLPQEDLCQALGYSPNLKYQSDGGPGIKEIMQLLNGSQQPQKDRDEFFRAQVVYWCLGAIDAHAKNFSISIQP
QGGYQLTPLYDIISAYPIVEQKQLSFKKLKMAMALYGKNIHYKIDGIQKRHFIETAKYVKYSKIKAAQIFEQVIDRIDSA
IDEVSRQLPNHFPEKIADPIFKGMRAFKERMIST

Nucleotide

• Length: 1305 bp

>T76362 NZ_CP021266:c68941-67637 [Legionella pneumophila subsp. pneumophila]
ATGGTAATGCGTAAATTATACATATTGATGAACAATGAGCTCGTGGGGGAGCTTACCCAGGATAATGCAGGTGGTTTGAG
TTTTTCCTACGTTGCTGACTGGATTTCCAAACCGCATTCACGCCCAGTTTCTTTATCGTTACCGTTAGTAAATCAAAAAT
TTTCAGGAGAAGTTGTTTATAACTTTTTTGATAATTTACTTCCCGATAATCCACAGATAAGAGCCAGAATTCAAGCTCGT
TTTCAGGCAAAATCATCGCGACCGTTTGATTTATTGGCGTTAATTGGCAGTGATTGTGTTGGTGCAATACAAATTGTTGA
ACGTATTCCTCAGACACAGCGTCTAAGCATTGAAAGTCACGTTGTCAGTGAGGCCGAGATAGCTCGTACTTTACGTTCTT
ACCAAACCTCACCACTGGGAATGGATAAAGAGGATGATGAGTTTAGAATCTCAATAGCCGGTGCTCAGGAAAAAACGGCT
TTTCTATATTACCAAAATGAATGGCATAAACCATTTGGACCTACACCAACAACTCACATATTCAAATTACCTATTGGATA
TTTACCACATCAAGGAATTGATTTAAGTGAGAGTTGTGAAAATGAGCATCTCTGTTTACTACTAGCCAGCAAATTTGGTT
TAGATGTTGCAAAATCAAAAATCTTACAATTCGAAGATGAAAAAGTGCTGGTTGTCGAAAGATTTGATAGAAAATGGAGT
CGAGATAACCGTTATATACTCAGGTTACCGCAAGAAGACCTATGCCAAGCGTTGGGTTATTCACCAAATTTAAAATATCA
GTCAGATGGTGGCCCCGGTATTAAAGAAATTATGCAGCTACTTAATGGCTCACAGCAGCCTCAAAAAGACAGAGATGAAT
TTTTCAGAGCACAAGTTGTTTACTGGTGCCTTGGTGCTATTGATGCGCATGCCAAAAATTTCAGTATAAGTATACAACCA
CAAGGCGGTTACCAGCTCACCCCCTTATACGACATCATTTCAGCCTATCCTATCGTGGAACAAAAACAGCTATCCTTTAA
GAAATTGAAAATGGCAATGGCTTTATATGGTAAAAATATTCATTACAAGATTGACGGTATTCAAAAAAGACACTTTATTG
AAACAGCAAAATACGTTAAATATTCTAAAATTAAAGCAGCTCAGATTTTTGAACAAGTGATTGACCGTATTGATTCTGCT
ATCGATGAGGTCTCAAGACAATTACCAAACCACTTCCCAGAAAAGATTGCAGATCCAATCTTCAAAGGGATGAGAGCTTT
TAAGGAGCGAATGATATCCACATAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9096.40 Da
• Isoelectric Point: 5.0774

>AT76362 WP_014840730.1 NZ_CP021266:c69174-68935 [Legionella pneumophila subsp. pneumophila]
MIISSAKDLALYLNDKRKQLKMSQSEVADLVGLKQDTISKFENSPDNSRIDTLFRILSALNLNMSLVEKDQKTHEEIEW

Nucleotide

• Length: 240 bp

>AT76362 NZ_CP021266:c69174-68935 [Legionella pneumophila subsp. pneumophila]
ATGATCATTAGCTCAGCAAAAGATTTAGCATTATATTTAAATGACAAGCGCAAACAACTCAAAATGAGTCAATCTGAAGT
TGCCGATTTAGTCGGCTTAAAACAGGATACCATATCAAAGTTTGAAAATAGCCCCGATAATAGTCGAATTGATACCTTAT
TTAGAATATTGTCCGCCCTAAATTTAAATATGAGCCTTGTTGAAAAAGATCAAAAAACTCATGAAGAGATAGAATGGTAA