Detailed information of TA system TA076345

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 2669940..2671374
• Replicon: chromosome
• Accession: NZ_CP021258
• Organism: Legionella pneumophila subsp. fraseri strain D-5744

Toxin (Protein)

• Gene name: HipA1
• Uniprot ID: A0A2S6EXC5
• Locus tag: CA935_RS11910
• Protein ID: WP_032830191.1
• Coordinates: 2670154..2671374 (+)
• Length: 407 a.a.

Antitoxin (Protein)

• Gene name: HipB1
• Uniprot ID: -
• Locus tag: CA935_RS11905
• Protein ID: WP_027227407.1
• Coordinates: 2669940..2670167 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA935_RS11880	2665735..2666601	-	867	WP_027227403.1	transposase	-
CA935_RS11885	2666663..2666851	-	189	WP_071974648.1	helix-turn-helix domain-containing protein	-
CA935_RS11890	2667040..2667828	+	789	WP_027227404.1	hypothetical protein	-
CA935_RS11895	2667893..2668303	-	411	Protein_2303	transposase family protein	-
CA935_RS11900	2668422..2669444	-	1023	WP_027227406.1	hypothetical protein	-
CA935_RS11905	2669940..2670167	+	228	WP_027227407.1	helix-turn-helix transcriptional regulator	Antitoxin
CA935_RS11910	2670154..2671374	+	1221	WP_032830191.1	type II toxin-antitoxin system HipA family toxin	Toxin
CA935_RS11915	2671409..2671765	-	357	WP_027227408.1	preprotein translocase	-
CA935_RS11920	2671805..2672710	-	906	WP_027227409.1	LysR family transcriptional regulator	-
CA935_RS11925	2672801..2673661	+	861	WP_027227410.1	DUF2608 domain-containing protein	-
CA935_RS11930	2674013..2674277	-	265	Protein_2310	hypothetical protein	-
CA935_RS11935	2674325..2674636	-	312	WP_027227411.1	cupin	-
CA935_RS11940	2674652..2674900	-	249	WP_031301595.1	hypothetical protein	-
CA935_RS11945	2675207..2675680	+	474	WP_027227413.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (147-380)

Antitoxin

• Transcription regulator, HipB-like (7-63)

Sequences

Toxin

Protein

• Length: 407 a.a.
• Molecular weight: 45374.54 Da
• Isoelectric Point: 8.3174

>T76345 WP_032830191.1 NZ_CP021258:2670154-2671374 [Legionella pneumophila subsp. fraseri]
MKNHKLDIYLNQTLAGQLRIDTHGEMSFVYDEGYLENNKNLPLSHSLPLQQAPYSTKQCRPFFSGLLPEAHMRTAIARQL
GISEKNDFALLAAIGGECAGAVMILPQDTSITQLKPDYKIIKKQDVLEILQTMHQRPMIVGEDGIRLSLAGAQDKLPVAI
IEGNLAIPMNGAPSTHILKPINRDFPSLIENESFCLNLAQKIGLNAVDASIHYADGTPYLLVKRYDRVETKQGIQRLHQE
DFCQAMGISPEMKYQREGGPQISECFGLIREISSLPVLDIKELLQGILFNVIIGNNDAHSKNFSLLYHGSKVRLAPFYDL
ISTVYYPNLASKMAMKVGSKYDFDGLFPRHFIQMAEEARLSGALVRKEVLNLISKIQKNITASPFTSIILQRADKLFQKF
QKASTG

Nucleotide

• Length: 1221 bp

>T76345 NZ_CP021258:2670154-2671374 [Legionella pneumophila subsp. fraseri]
ATGAAAAATCATAAACTTGATATCTATCTTAACCAAACACTAGCAGGCCAATTACGTATCGATACGCATGGCGAGATGTC
TTTTGTGTATGATGAAGGCTACCTGGAAAACAATAAAAATCTCCCGTTGTCACACTCCCTTCCCCTTCAACAAGCCCCTT
ACTCAACGAAGCAATGCCGTCCGTTTTTTAGCGGACTATTGCCTGAAGCCCATATGCGTACTGCTATTGCCCGTCAATTG
GGTATCAGTGAAAAAAATGATTTTGCCTTATTAGCAGCTATTGGTGGTGAGTGTGCCGGAGCTGTGATGATATTACCTCA
AGATACATCCATAACTCAGCTAAAGCCAGACTATAAAATTATCAAAAAACAGGATGTATTAGAAATCCTGCAAACCATGC
ATCAAAGACCCATGATAGTCGGTGAGGATGGAATTCGTTTATCCCTTGCTGGAGCTCAGGATAAACTTCCAGTTGCCATT
ATTGAAGGAAATCTCGCAATACCAATGAACGGTGCACCGAGTACTCATATTTTAAAACCAATTAATCGAGACTTTCCCTC
CTTAATTGAAAATGAATCTTTCTGCCTTAATCTGGCACAAAAAATTGGATTAAATGCTGTGGACGCATCAATCCATTATG
CTGATGGTACCCCTTATCTGTTGGTTAAACGTTATGATCGGGTTGAGACAAAGCAAGGAATCCAACGTCTTCATCAAGAA
GATTTTTGTCAGGCAATGGGCATTAGCCCTGAAATGAAATATCAGCGAGAAGGCGGCCCCCAAATTAGTGAGTGCTTTGG
TTTGATAAGAGAGATATCAAGCCTCCCTGTCCTTGACATAAAAGAATTATTACAAGGGATTCTGTTTAATGTCATTATTG
GCAACAATGATGCACATAGCAAAAACTTCTCTTTGCTCTATCATGGTTCAAAAGTTCGTTTAGCACCTTTCTACGATCTG
ATTTCAACCGTATATTATCCTAATCTTGCCAGTAAAATGGCGATGAAAGTTGGAAGTAAATATGATTTTGATGGCCTATT
TCCACGCCACTTTATACAAATGGCAGAGGAGGCTCGTTTATCGGGTGCCTTAGTGCGTAAAGAAGTCTTGAATCTAATTA
GCAAAATTCAAAAGAACATAACCGCCAGCCCCTTTACCAGCATCATTTTGCAACGTGCTGACAAATTATTTCAAAAGTTC
CAGAAGGCTTCAACAGGATAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8467.85 Da
• Isoelectric Point: 9.1200

>AT76345 WP_027227407.1 NZ_CP021258:2669940-2670167 [Legionella pneumophila subsp. fraseri]
MIQSPENIGRYIRKVRKELNVTQKDLALTAGTGLRFIIDLENGKPTCQIGKMLQVLQVLGIQLNLSHTDFENEKS

Nucleotide

• Length: 228 bp

>AT76345 NZ_CP021258:2669940-2670167 [Legionella pneumophila subsp. fraseri]
ATGATTCAATCTCCAGAAAATATAGGACGTTATATTCGCAAAGTTCGTAAAGAACTTAATGTTACCCAAAAAGATTTGGC
TTTAACTGCTGGAACTGGATTGCGTTTTATTATCGATTTGGAAAATGGAAAGCCCACTTGCCAAATTGGAAAAATGCTGC
AAGTACTACAAGTACTTGGGATTCAACTCAACTTATCTCATACTGATTTCGAAAATGAAAAATCATAA

Structures

Toxin

• AlphaFold DB: A0A2S6EXC5