Detailed information of TA system TA076344

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 664711..666185
• Replicon: chromosome
• Accession: NZ_CP021257
• Organism: Legionella pneumophila subsp. fraseri strain Lansing 3

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: A0A0W1AGC7
• Locus tag: CA936_RS02995
• Protein ID: WP_028378775.1
• Coordinates: 665316..666185 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: -
• Locus tag: CA936_RS02990
• Protein ID: WP_028378776.1
• Coordinates: 664711..665319 (+)
• Length: 203 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA936_RS16665	660278..660997	-	720	WP_202863481.1	UvrD-helicase domain-containing protein	-
CA936_RS02980	660984..662825	-	1842	WP_027227389.1	AAA family ATPase	-
CA936_RS02985	663023..664198	+	1176	WP_028378827.1	ISL3 family transposase	-
CA936_RS02990	664711..665319	+	609	WP_028378776.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
CA936_RS02995	665316..666185	+	870	WP_028378775.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
CA936_RS03000	666227..666664	-	438	WP_028378774.1	conjugal transfer protein TraM	-
CA936_RS03005	666664..667389	-	726	WP_028378773.1	conjugal transfer protein TraL	-
CA936_RS03010	667389..667733	-	345	WP_028378772.1	TraK family protein	-
CA936_RS03015	668129..668482	+	354	WP_028378771.1	conjugal transfer transcriptional regulator TraJ	-
CA936_RS03020	668479..670077	+	1599	WP_080274386.1	relaxase/mobilization nuclease domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (35-260)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (10-56)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32560.44 Da
• Isoelectric Point: 6.9833

>T76344 WP_028378775.1 NZ_CP021257:665316-666185 [Legionella pneumophila subsp. fraseri]
MTKNIGESVRSRLKNIAVKEGSDFNAVLTRYGLERLLYRIGESEYSKQFLLKGALLFNLWYDMPHRPTKDIDLLGFGDND
LAYIKQTFSKICRISADDGISFLSESVTADTIKKDGGYTGARVELFGELAKAKIKIQIDIGYGDAVTPGPIDAHYPVLLS
DLPAPKIRTYPIYTVIAEKLHAIALLGMTNSRLKDYLDLYVLLGNEQIDNQILAKAIHATFNRRSMDLPEELPIGLTDEF
ANDPSRESMWKAFLRKNELEQKSLTEVIAVIRNLIQVPYSLSTHPNFSR

Nucleotide

• Length: 870 bp

>T76344 NZ_CP021257:665316-666185 [Legionella pneumophila subsp. fraseri]
ATGACAAAAAATATTGGTGAGTCTGTACGCTCTCGTTTGAAGAATATAGCTGTTAAAGAAGGTTCTGATTTTAATGCGGT
ATTGACTCGTTATGGTCTAGAGCGTTTGTTATATCGAATTGGCGAATCAGAATACTCTAAACAGTTTTTGTTGAAAGGTG
CCTTACTATTTAACTTGTGGTATGACATGCCTCATCGCCCAACTAAAGATATTGATTTATTGGGGTTTGGTGATAATGAT
CTAGCGTATATTAAACAAACATTCAGTAAAATTTGCAGAATCTCTGCAGATGATGGAATAAGTTTCCTGTCTGAATCAGT
GACGGCCGATACTATCAAAAAAGATGGAGGTTATACTGGGGCAAGAGTTGAGTTATTTGGGGAATTGGCGAAAGCAAAGA
TTAAAATACAAATTGATATAGGGTATGGTGATGCAGTTACCCCTGGGCCAATAGATGCCCATTATCCCGTACTTCTTAGT
GATCTTCCTGCCCCTAAAATCCGTACTTATCCAATTTATACGGTAATTGCAGAAAAACTGCACGCAATTGCATTATTGGG
CATGACTAATAGTCGATTAAAAGACTACTTGGATCTTTATGTATTATTAGGTAATGAACAGATAGATAATCAAATCTTGG
CTAAAGCGATACATGCTACATTTAATCGTCGAAGTATGGATCTTCCTGAAGAGCTACCTATTGGCCTGACCGATGAGTTT
GCTAATGACCCAAGCAGGGAGTCAATGTGGAAAGCCTTTTTGAGAAAAAATGAACTAGAGCAGAAATCATTAACTGAAGT
TATTGCTGTGATCAGAAATTTGATTCAAGTGCCATATTCTTTGTCAACACACCCTAATTTTAGTAGATAA

Antitoxin

Protein

• Length: 203 a.a.
• Molecular weight: 22968.85 Da
• Isoelectric Point: 9.0310

>AT76344 WP_028378776.1 NZ_CP021257:664711-665319 [Legionella pneumophila subsp. fraseri]
MKTEATYKQKVLQMASKSGIVRMSDLTERGITRATISRLVSENKLEKLASGLYCLPETEFSEKESLVIIASRVPQAVFCL
LTALQIHDLTTQLPRKVWIAMPKGSHAPKMDYPPLKMVQYSDEAFSEGIEIIESDNIKLRVYNFAKTIADCFKHRNKIGL
DVAIEALKEAYTKNKVTVDELWHYAKICRVANVMRPYIEAIQ

Nucleotide

• Length: 609 bp

>AT76344 NZ_CP021257:664711-665319 [Legionella pneumophila subsp. fraseri]
ATGAAGACAGAAGCTACCTATAAACAAAAAGTACTCCAGATGGCTAGTAAATCGGGCATTGTTCGAATGTCCGATCTAAC
CGAAAGGGGGATCACTAGAGCGACCATTTCGAGGCTTGTTAGCGAAAACAAACTTGAAAAACTCGCTTCTGGTCTCTATT
GCTTGCCCGAGACGGAGTTTTCAGAAAAAGAAAGCCTGGTTATTATAGCAAGCCGAGTACCCCAAGCAGTGTTTTGCCTG
CTAACTGCTTTGCAGATTCATGATCTAACAACACAGCTACCTCGAAAAGTATGGATAGCTATGCCAAAAGGGAGTCATGC
GCCAAAAATGGATTATCCTCCTCTAAAAATGGTGCAATATTCAGATGAAGCCTTTAGTGAAGGAATTGAAATTATAGAAT
CAGATAATATTAAGCTAAGGGTGTATAACTTTGCCAAGACAATTGCAGATTGTTTCAAACATCGCAATAAAATTGGACTT
GATGTGGCTATTGAAGCGCTCAAAGAGGCTTATACAAAAAATAAAGTCACAGTTGATGAACTATGGCATTATGCGAAAAT
TTGTCGCGTTGCAAACGTAATGCGTCCTTATATTGAGGCAATTCAATGA

Structures

Toxin

• AlphaFold DB: A0A0W1AGC7