Detailed information of TA system TA076338

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 514964..515441
• Replicon: chromosome
• Accession: NZ_CP021255
• Organism: Desulfobulbus oralis strain HOT041/ORNL

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: -
• Locus tag: CAY53_RS02085
• Protein ID: WP_104935724.1
• Coordinates: 515172..515441 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: -
• Locus tag: CAY53_RS02080
• Protein ID: WP_104935723.1
• Coordinates: 514964..515188 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CAY53_RS02050	510113..510556	-	444	WP_104935618.1	IS200/IS605 family transposase	-
CAY53_RS02055	511020..511759	+	740	Protein_411	IS5 family transposase	-
CAY53_RS02060	512183..513439	+	1257	WP_104935672.1	ISL3 family transposase	-
CAY53_RS02075	513735..514591	+	857	Protein_413	site-specific integrase	-
CAY53_RS02080	514964..515188	+	225	WP_104935723.1	TraY domain-containing protein	Antitoxin
CAY53_RS02085	515172..515441	+	270	WP_104935724.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CAY53_RS02090	515478..515831	+	354	WP_104935725.1	conjugal transfer protein TraJ	-
CAY53_RS02095	515828..518137	+	2310	WP_104935726.1	relaxase/mobilization nuclease domain-containing protein	-
CAY53_RS12440	518205..518618	-	414	WP_146106369.1	hypothetical protein	-
CAY53_RS12445	518661..519260	-	600	WP_146106370.1	hypothetical protein	-
CAY53_RS02105	519235..519474	-	240	WP_017866015.1	hypothetical protein	-
CAY53_RS02110	519505..520053	-	549	WP_104935728.1	tyrosine-type recombinase/integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	514964..520053	5089

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-88)

Antitoxin

• Relaxosome protein TraY (4-49)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10363.07 Da
• Isoelectric Point: 10.2161

>T76338 WP_104935724.1 NZ_CP021255:515172-515441 [Desulfobulbus oralis]
MGWKIELARAAERDLDKLDPQVARRILVFLHGRLAELDDPRSIGEPLKGSKLGEFWKYRVGDYRVISSIEDKVLTILVVR
IGNRREVYR

Nucleotide

• Length: 270 bp

>T76338 NZ_CP021255:515172-515441 [Desulfobulbus oralis]
ATGGGCTGGAAAATTGAGCTTGCCCGCGCTGCAGAACGTGATTTGGATAAACTCGATCCTCAGGTGGCCAGACGTATTCT
TGTTTTTCTGCATGGCCGTCTGGCTGAACTGGACGACCCACGCAGTATCGGTGAGCCACTCAAAGGATCGAAGCTGGGTG
AATTCTGGAAGTACCGCGTCGGTGATTACAGGGTAATCAGCAGCATCGAGGACAAGGTGCTGACAATCCTCGTTGTGAGA
ATTGGCAATCGTCGAGAGGTCTACAGATAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8402.63 Da
• Isoelectric Point: 4.4633

>AT76338 WP_104935723.1 NZ_CP021255:514964-515188 [Desulfobulbus oralis]
MLAIRLPAEIEARLDAMAKATGRTKTFYARQAILEYLDDLEDLYLAEQRLSEIRTGASEVVALEEVVKRYGLEN

Nucleotide

• Length: 225 bp

>AT76338 NZ_CP021255:514964-515188 [Desulfobulbus oralis]
ATGCTCGCAATCAGACTGCCCGCTGAGATAGAAGCCCGGCTTGACGCCATGGCAAAGGCCACTGGCCGAACCAAAACATT
CTATGCCCGGCAAGCCATTCTCGAATACCTGGATGATCTTGAAGACCTGTATCTGGCTGAGCAACGATTGAGCGAGATCA
GAACCGGAGCGTCGGAGGTTGTGGCTCTTGAGGAAGTGGTGAAGCGCTATGGGCTGGAAAATTGA