Detailed information of TA system TA076335

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 1307161..1307690
• Replicon: chromosome
• Accession: NZ_CP021248
• Organism: Novosphingobium sp. KA1

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: CA833_RS23600
• Protein ID: WP_142635895.1
• Coordinates: 1307161..1307406 (+)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: CA833_RS23605
• Protein ID: WP_142635897.1
• Coordinates: 1307403..1307690 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA833_RS23575	1302407..1302670	-	264	Protein_1148	transposase	-
CA833_RS23580	1302731..1303033	+	303	WP_207081304.1	CPBP family intramembrane metalloprotease	-
CA833_RS23585	1303355..1304458	-	1104	WP_207080411.1	BamA/TamA family outer membrane protein	-
CA833_RS23590	1304725..1305996	+	1272	WP_207080412.1	BamA/TamA family outer membrane protein	-
CA833_RS23595	1306260..1306892	+	633	WP_207080413.1	tyrosine-type recombinase/integrase	-
CA833_RS23600	1307161..1307406	+	246	WP_142635895.1	BrnT family toxin	Toxin
CA833_RS23605	1307403..1307690	+	288	WP_142635897.1	BrnA antitoxin family protein	Antitoxin
CA833_RS23610	1308290..1308814	-	525	WP_207080414.1	hypothetical protein	-
CA833_RS23615	1308965..1310524	-	1560	WP_207081305.1	MFS transporter	-
CA833_RS23620	1310690..1311805	-	1116	WP_142638489.1	glycoside hydrolase family 88 protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-76)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (21-92)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 8697.12 Da
• Isoelectric Point: 5.7551

>T76335 WP_142635895.1 NZ_CP021248:1307161-1307406 [Novosphingobium sp. KA1]
MTIVWDEPKRQANLVKHGIDFADVGEGFFLSALVIPAKDGRFAAIGEMNGTITVIFAVLGTEGVSIISARPASITERRLL
P

Nucleotide

• Length: 246 bp

>T76335 NZ_CP021248:1307161-1307406 [Novosphingobium sp. KA1]
ATGACAATCGTGTGGGATGAACCAAAACGGCAAGCGAACCTCGTCAAGCACGGGATCGACTTCGCCGATGTAGGCGAAGG
GTTCTTCCTCTCCGCCCTGGTCATCCCCGCGAAGGACGGACGCTTTGCCGCCATCGGTGAGATGAACGGCACGATCACGG
TGATTTTCGCTGTGCTGGGCACCGAAGGCGTCTCTATCATCTCTGCTCGCCCGGCCAGCATTACGGAACGGAGGCTCCTG
CCATGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10502.89 Da
• Isoelectric Point: 8.6403

>AT76335 WP_142635897.1 NZ_CP021248:1307403-1307690 [Novosphingobium sp. KA1]
MTDPKHAAPGYTKADMDVVSDNPEWTVEDFARAKPFAEAFPDLAKTMRARGPQKAPKKVSTTLRLSPEVIEHFKSGGPGW
QSRIDAALRDWVAAH

Nucleotide

• Length: 288 bp

>AT76335 NZ_CP021248:1307403-1307690 [Novosphingobium sp. KA1]
ATGACCGACCCGAAACACGCCGCCCCCGGATACACCAAGGCCGATATGGATGTGGTCAGCGATAACCCCGAATGGACGGT
TGAGGACTTCGCCAGGGCAAAGCCTTTCGCTGAGGCCTTCCCGGACCTAGCAAAGACGATGCGCGCACGCGGCCCGCAGA
AAGCTCCGAAAAAGGTCAGCACCACGCTGCGCCTTTCGCCCGAGGTGATCGAGCATTTCAAATCCGGCGGCCCTGGTTGG
CAGTCACGGATCGATGCCGCTCTCAGGGATTGGGTAGCAGCACACTGA