Detailed information of TA system TA076334

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 568007..568645
• Replicon: chromosome
• Accession: NZ_CP021248
• Organism: Novosphingobium sp. KA1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CA833_RS20450
• Protein ID: WP_010338821.1
• Coordinates: 568244..568645 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0J9FLQ9
• Locus tag: CA833_RS20445
• Protein ID: WP_010338822.1
• Coordinates: 568007..568240 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA833_RS20410	563803..564216	+	414	WP_010336366.1	PRTRC system protein E	-
CA833_RS20415	564271..564477	+	207	WP_010336367.1	hypothetical protein	-
CA833_RS20420	564489..564716	+	228	WP_010336368.1	hypothetical protein	-
CA833_RS20425	564820..565677	+	858	Protein_518	hypothetical protein	-
CA833_RS20430	565689..566519	+	831	WP_010338825.1	PRTRC system protein B	-
CA833_RS20435	566516..567270	+	755	Protein_520	PRTRC system protein A	-
CA833_RS20440	567165..567971	+	807	WP_010338823.1	PRTRC system ThiF family protein	-
CA833_RS20445	568007..568240	+	234	WP_010338822.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CA833_RS20450	568244..568645	+	402	WP_010338821.1	type II toxin-antitoxin system VapC family toxin	Toxin
CA833_RS20455	568837..569694	+	858	WP_207080987.1	hypothetical protein	-
CA833_RS20460	569713..570171	+	459	WP_207080988.1	hypothetical protein	-
CA833_RS20465	570652..571194	+	543	WP_207080989.1	hypothetical protein	-
CA833_RS20470	571440..571931	+	492	WP_207080990.1	hypothetical protein	-
CA833_RS20475	571928..572416	+	489	WP_207080991.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	516238..700050	183812

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-123)

Antitoxin

• SpoVT-AbrB domain (10-55)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14379.59 Da
• Isoelectric Point: 4.9102

>T76334 WP_010338821.1 NZ_CP021248:568244-568645 [Novosphingobium sp. KA1]
MFLLDTNILSDLIRQPDGMVAHAIADVGEDAVATSIIVAGELRYGAEKRGSPRLKSKIEDLLSLIAVLPLKGDADACYGR
LRADLERRGTPIGANDMLIAAHALAIGATLVTDNIREFERVDGLQLVNWLRTT

Nucleotide

• Length: 402 bp

>T76334 NZ_CP021248:568244-568645 [Novosphingobium sp. KA1]
ATGTTCCTGCTCGATACGAATATCCTGTCGGACCTGATCCGGCAGCCCGATGGCATGGTCGCCCACGCTATCGCCGACGT
CGGCGAGGACGCGGTTGCGACCAGCATCATCGTTGCCGGCGAACTGCGCTATGGCGCAGAAAAGCGCGGTTCGCCGCGCC
TGAAAAGCAAGATCGAGGATTTGCTGTCTCTCATTGCGGTGCTGCCGCTCAAGGGCGATGCCGACGCCTGCTATGGGCGT
CTGCGTGCCGACCTCGAGCGTCGTGGCACGCCGATCGGCGCTAACGACATGCTGATCGCCGCCCACGCGCTCGCAATCGG
GGCGACCCTCGTTACCGACAACATCCGCGAATTTGAGCGGGTCGATGGCCTGCAACTGGTGAACTGGCTCCGAACCACCT
GA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8809.19 Da
• Isoelectric Point: 4.8723

>AT76334 WP_010338822.1 NZ_CP021248:568007-568240 [Novosphingobium sp. KA1]
MNLERHVKLFKNGRSQAVRIPREFELPGEDAIIRKEGSRLIIEPARPRSLLAVLKTWEPLDEEIGPIEDLPAEPVDI

Nucleotide

• Length: 234 bp

>AT76334 NZ_CP021248:568007-568240 [Novosphingobium sp. KA1]
ATGAACCTCGAGCGTCACGTCAAACTGTTCAAGAACGGCCGTAGCCAGGCGGTCCGCATTCCCCGTGAGTTCGAACTTCC
GGGCGAGGACGCCATTATCCGCAAGGAAGGCTCCCGCCTGATCATCGAACCCGCCCGTCCGCGCTCCCTCCTTGCCGTTC
TCAAGACATGGGAGCCGCTCGATGAAGAGATCGGCCCGATCGAGGACCTGCCCGCAGAACCGGTCGACATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0J9FLQ9