Detailed information of TA system TA076333

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 1754177..1754706
• Replicon: chromosome
• Accession: NZ_CP021247
• Organism: Novosphingobium sp. KA1

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: CA833_RS08625
• Protein ID: WP_142635895.1
• Coordinates: 1754461..1754706 (-)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: CA833_RS08620
• Protein ID: WP_142635897.1
• Coordinates: 1754177..1754464 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA833_RS08600	1749778..1752003	+	2226	WP_207079809.1	tetratricopeptide repeat protein	-
CA833_RS08605	1752045..1752440	+	396	WP_142635908.1	hypothetical protein	-
CA833_RS08615	1753740..1754009	+	270	WP_207079811.1	hypothetical protein	-
CA833_RS08620	1754177..1754464	-	288	WP_142635897.1	BrnA antitoxin family protein	Antitoxin
CA833_RS08625	1754461..1754706	-	246	WP_142635895.1	BrnT family toxin	Toxin
CA833_RS08630	1754958..1756295	-	1338	WP_207079812.1	methyltransferase domain-containing protein	-
CA833_RS08635	1756543..1757232	-	690	WP_207080020.1	TonB family protein	-
CA833_RS08640	1757618..1759213	-	1596	WP_207079813.1	RNA polymerase factor sigma-54	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-76)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (21-92)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 8697.12 Da
• Isoelectric Point: 5.7551

>T76333 WP_142635895.1 NZ_CP021247:c1754706-1754461 [Novosphingobium sp. KA1]
MTIVWDEPKRQANLVKHGIDFADVGEGFFLSALVIPAKDGRFAAIGEMNGTITVIFAVLGTEGVSIISARPASITERRLL
P

Nucleotide

• Length: 246 bp

>T76333 NZ_CP021247:c1754706-1754461 [Novosphingobium sp. KA1]
ATGACAATCGTGTGGGATGAACCAAAACGGCAAGCGAACCTCGTCAAGCACGGGATCGACTTCGCCGATGTAGGCGAAGG
GTTCTTCCTCTCCGCCCTGGTCATCCCCGCGAAGGACGGACGCTTTGCCGCCATCGGTGAGATGAACGGCACGATCACGG
TGATTTTCGCTGTGCTGGGCACCGAAGGCGTCTCTATCATCTCTGCTCGCCCGGCCAGCATTACGGAACGGAGGCTCCTG
CCATGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10502.89 Da
• Isoelectric Point: 8.6403

>AT76333 WP_142635897.1 NZ_CP021247:c1754464-1754177 [Novosphingobium sp. KA1]
MTDPKHAAPGYTKADMDVVSDNPEWTVEDFARAKPFAEAFPDLAKTMRARGPQKAPKKVSTTLRLSPEVIEHFKSGGPGW
QSRIDAALRDWVAAH

Nucleotide

• Length: 288 bp

>AT76333 NZ_CP021247:c1754464-1754177 [Novosphingobium sp. KA1]
ATGACCGACCCGAAACACGCCGCCCCCGGATACACCAAAGCCGATATGGATGTGGTCAGCGATAACCCCGAATGGACGGT
TGAGGACTTCGCCAGGGCAAAGCCTTTCGCTGAGGCCTTCCCGGACCTAGCAAAGACGATGCGCGCACGCGGCCCGCAGA
AAGCTCCGAAAAAGGTCAGCACCACGCTGCGCCTTTCGCCTGAGGTGATCGAGCATTTCAAATCCGGCGGCCCTGGCTGG
CAGTCACGGATCGATGCCGCTCTCAGGGATTGGGTGGCAGCACACTGA