Detailed information of TA system TA076332

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 1350..1963
• Replicon: chromosome
• Accession: NZ_CP021247
• Organism: Novosphingobium sp. KA1

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: CA833_RS00010
• Protein ID: WP_207078824.1
• Coordinates: 1350..1640 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: CA833_RS00015
• Protein ID: WP_207078825.1
• Coordinates: 1676..1963 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA833_RS00005	168..863	+	696	WP_207078823.1	helix-turn-helix domain-containing protein	-
CA833_RS00010	1350..1640	+	291	WP_207078824.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CA833_RS00015	1676..1963	+	288	WP_207078825.1	putative addiction module antidote protein	Antitoxin
CA833_RS00020	2080..2682	+	603	WP_207078826.1	hypothetical protein	-
CA833_RS00025	2729..3841	+	1113	WP_207078827.1	ImmA/IrrE family metallo-endopeptidase	-
CA833_RS00030	3834..4901	-	1068	WP_207078828.1	phage portal protein	-
CA833_RS00035	4898..6748	-	1851	WP_207078829.1	terminase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1350..39307	37957

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (2-94)

Antitoxin

• Uncharacterized protein HI1420 (4-91)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10695.33 Da
• Isoelectric Point: 10.3721

>T76332 WP_207078824.1 NZ_CP021247:1350-1640 [Novosphingobium sp. KA1]
METQQTPLFANWFNGLRDRKAQSKIAGRIARIELGLMGDVKSVGDGVSEVRIDFGPGYRLYFTRRGEQLIILLMGGDKSS
QQRDIAKAKELAAQIP

Nucleotide

• Length: 291 bp

>T76332 NZ_CP021247:1350-1640 [Novosphingobium sp. KA1]
ATGGAAACGCAGCAGACTCCGCTCTTCGCGAACTGGTTTAACGGCCTTAGGGACCGCAAGGCACAGTCGAAGATCGCGGG
TCGCATCGCCCGAATTGAACTCGGGCTAATGGGTGATGTGAAGTCGGTTGGTGATGGCGTTTCCGAGGTGCGTATCGATT
TCGGTCCCGGCTACCGCCTCTATTTCACCCGCCGGGGCGAGCAATTGATTATTCTGCTGATGGGTGGCGACAAGTCTAGC
CAGCAGCGGGATATCGCAAAGGCCAAGGAACTGGCCGCTCAGATACCGTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10276.81 Da
• Isoelectric Point: 4.6510

>AT76332 WP_207078825.1 NZ_CP021247:1676-1963 [Novosphingobium sp. KA1]
MSIELTRFDAAEYLSEPEDQAELLADALETGDAHIVAKAIGIVARAHGMTELAKETGIKRQQLYRTLGPDGNPTLETMLK
VLPALGLKIRFEAAA

Nucleotide

• Length: 288 bp

>AT76332 NZ_CP021247:1676-1963 [Novosphingobium sp. KA1]
ATGTCTATTGAACTCACCCGCTTTGATGCGGCCGAATATCTTTCTGAGCCCGAAGATCAGGCAGAACTGCTCGCAGATGC
GCTCGAAACCGGCGATGCCCACATCGTGGCAAAGGCGATCGGCATCGTTGCCCGCGCCCACGGGATGACGGAATTGGCAA
AAGAAACCGGCATCAAGCGCCAACAGCTGTACCGGACGCTGGGCCCTGACGGGAACCCGACGCTGGAAACCATGCTGAAG
GTACTGCCGGCGCTTGGCCTGAAGATTCGGTTCGAAGCGGCCGCCTAA