Detailed information of TA system TA076331

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-RHH
• Location: 3700403..3700977
• Replicon: chromosome
• Accession: NZ_CP021219
• Organism: Sinorhizobium meliloti RU11/001

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q92QN4
• Locus tag: SMRU11_RS37540
• Protein ID: WP_010969154.1
• Coordinates: 3700612..3700977 (+)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q92QN3
• Locus tag: SMRU11_RS37535
• Protein ID: WP_010969155.1
• Coordinates: 3700403..3700618 (+)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SMRU11_RS37515	3696329..3697015	-	687	WP_010969157.1	ABC transporter ATP-binding protein	-
SMRU11_RS37520	3697033..3698343	-	1311	WP_014529722.1	lipoprotein-releasing ABC transporter permease subunit	-
SMRU11_RS37525	3698364..3699692	-	1329	WP_003531873.1	proline--tRNA ligase	-
SMRU11_RS37530	3700108..3700335	+	228	WP_017264047.1	hypothetical protein	-
SMRU11_RS37535	3700403..3700618	+	216	WP_010969155.1	ribbon-helix-helix protein, CopG family	Antitoxin
SMRU11_RS37540	3700612..3700977	+	366	WP_010969154.1	type II toxin-antitoxin system VapC family toxin	Toxin
SMRU11_RS37545	3701064..3701333	-	270	WP_014526683.1	DUF1467 family protein	-
SMRU11_RS37550	3701473..3701877	-	405	WP_010969152.1	methylmalonyl-CoA epimerase	-
SMRU11_RS37555	3701936..3703603	-	1668	WP_010969151.1	ribonuclease J	-
SMRU11_RS37560	3703631..3704398	-	768	WP_014529723.1	biotin--[acetyl-CoA-carboxylase] ligase	-
SMRU11_RS37565	3704395..3705837	-	1443	WP_010969150.1	NADH-quinone oxidoreductase subunit NuoN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-106)

Antitoxin

• Ribbon-helix-helix protein, CopG (9-40)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13592.57 Da
• Isoelectric Point: 5.1787

>T76331 WP_010969154.1 NZ_CP021219:3700612-3700977 [Sinorhizobium meliloti RU11/001]
MVGALFDTNILIDHLNAVPQAHKELDRFENRAISIITWMEVMVGADAELVEPTRRFLDGFETIALNDEIANRAVTLRRAH
RIKLPDAVIWATAQTAGRLLVTRNTKDFPADDPGIREPYAV

Nucleotide

• Length: 366 bp

>T76331 NZ_CP021219:3700612-3700977 [Sinorhizobium meliloti RU11/001]
ATGGTAGGCGCACTGTTCGATACCAATATCCTTATCGATCATCTGAATGCGGTGCCTCAGGCGCACAAGGAACTTGACCG
TTTCGAAAACCGGGCCATCAGCATCATCACCTGGATGGAGGTGATGGTTGGAGCGGACGCCGAACTGGTTGAGCCGACCC
GGCGCTTCCTCGACGGTTTCGAAACGATCGCCTTGAACGATGAGATCGCCAACCGTGCGGTGACATTGCGCAGGGCGCAC
CGCATCAAGCTACCCGACGCGGTGATCTGGGCAACGGCGCAGACTGCCGGTCGTCTGCTCGTGACGCGCAATACCAAGGA
CTTTCCAGCCGACGACCCGGGGATACGCGAACCCTACGCCGTGTAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 8381.51 Da
• Isoelectric Point: 8.6052

>AT76331 WP_010969155.1 NZ_CP021219:3700403-3700618 [Sinorhizobium meliloti RU11/001]
MRALIDMNDTQVEALDTLAKRVRRSRAALIREAIDDYLNRHHREQIEDGFGLWGKRKVDGLAYQEKVRGEW

Nucleotide

• Length: 216 bp

>AT76331 NZ_CP021219:3700403-3700618 [Sinorhizobium meliloti RU11/001]
ATGCGAGCCTTGATCGATATGAACGATACCCAGGTCGAAGCCCTAGATACCCTCGCCAAGCGGGTGCGCCGGTCCCGTGC
GGCTCTGATCCGCGAAGCGATCGACGACTATCTCAACCGTCATCATCGCGAGCAGATCGAAGATGGCTTCGGTTTGTGGG
GCAAGCGCAAAGTTGACGGTCTGGCTTATCAGGAGAAGGTGCGCGGCGAATGGTAG

Structures

Toxin

• AlphaFold DB: Q92QN4

Antitoxin

• AlphaFold DB: Q92QN3