Detailed information of TA system TA076330

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB-HicA
• Location: 2804798..2805452
• Replicon: chromosome
• Accession: NZ_CP021219
• Organism: Sinorhizobium meliloti RU11/001

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: H0G1A3
• Locus tag: SMRU11_RS32820
• Protein ID: WP_003530010.1
• Coordinates: 2805264..2805452 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: Q92NX0
• Locus tag: SMRU11_RS32815
• Protein ID: WP_010969659.1
• Coordinates: 2804798..2805259 (-)
• Length: 154 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SMRU11_RS32790	2800933..2801376	+	444	WP_003529999.1	TIGR02594 family protein	-
SMRU11_RS32795	2801527..2802633	+	1107	WP_003530001.1	endonuclease	-
SMRU11_RS32800	2802636..2803385	+	750	WP_010969660.1	NUDIX hydrolase	-
SMRU11_RS32805	2803562..2804479	+	918	WP_003530005.1	PhzF family phenazine biosynthesis protein	-
SMRU11_RS32815	2804798..2805259	-	462	WP_010969659.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
SMRU11_RS32820	2805264..2805452	-	189	WP_003530010.1	type II toxin-antitoxin system HicA family toxin	Toxin
SMRU11_RS32825	2805629..2806399	+	771	WP_010969658.1	hypothetical protein	-
SMRU11_RS32830	2806580..2807551	+	972	WP_017265202.1	glycosyltransferase family 2 protein	-
SMRU11_RS32835	2807576..2808886	-	1311	WP_010969656.1	HlyD family type I secretion periplasmic adaptor subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-124)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 6971.15 Da
• Isoelectric Point: 11.0889

>T76330 WP_003530010.1 NZ_CP021219:c2805452-2805264 [Sinorhizobium meliloti RU11/001]
MKSGDIIAALQKDGWYEVATKGSHVQFKHPKKHGRVTVPHPKRDLPIGTLRSIEKQSGLKLR

Nucleotide

• Length: 189 bp

>T76330 NZ_CP021219:c2805452-2805264 [Sinorhizobium meliloti RU11/001]
ATGAAGAGCGGCGACATTATTGCAGCATTGCAGAAGGACGGATGGTACGAGGTTGCGACCAAGGGCAGCCACGTTCAATT
CAAACATCCGAAAAAGCACGGCCGGGTTACCGTTCCTCACCCGAAGAGGGATCTACCAATCGGTACCCTCAGGAGCATTG
AAAAGCAGTCCGGTTTGAAATTGAGGTAA

Antitoxin

Protein

• Length: 154 a.a.
• Molecular weight: 16293.17 Da
• Isoelectric Point: 4.3990

>AT76330 WP_010969659.1 NZ_CP021219:c2805259-2804798 [Sinorhizobium meliloti RU11/001]
MRNYIGLIHKDAESDYGVSFPDFSGVVTAGADLDDARAMAEEALALHIEGLVEDGEAIPEPSSLEVVMSDAENRDCVAIL
VAVKTEAKRAIRVNVTLPEGVLKQIDAFAEAHGLTRSGFLARAATHEIERANDGHDAYAESRLSALGTSSKNV

Nucleotide

• Length: 462 bp

>AT76330 NZ_CP021219:c2805259-2804798 [Sinorhizobium meliloti RU11/001]
ATGCGCAACTATATCGGATTGATCCACAAGGACGCCGAGAGCGATTACGGCGTTTCCTTTCCTGATTTTTCCGGCGTCGT
GACCGCCGGTGCCGACCTCGACGACGCGCGCGCTATGGCAGAGGAAGCCTTGGCCTTGCATATTGAAGGGCTGGTAGAGG
ACGGCGAGGCCATCCCGGAGCCCTCCTCCCTTGAGGTTGTCATGTCCGACGCCGAGAACAGGGATTGCGTCGCTATTCTC
GTCGCGGTGAAAACCGAGGCAAAAAGAGCCATTCGAGTGAACGTTACGCTTCCCGAAGGTGTCCTGAAGCAGATAGATGC
CTTCGCCGAAGCCCACGGCCTTACGCGGTCTGGATTTCTTGCTCGTGCTGCAACGCACGAGATCGAACGGGCAAATGACG
GCCACGACGCCTATGCGGAATCCCGCTTGTCGGCTTTGGGCACTTCCAGCAAAAATGTGTAA

Structures

Toxin

• AlphaFold DB: H0G1A3

Antitoxin

• AlphaFold DB: Q92NX0